Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,963,166 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,963,1660GC98.8% 745.3 / NA 324intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/4);  new base C (156/161);  total (157/167)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP0425831,963,1670CG98.8% 738.2 / NA 325intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/4);  new base G (156/163);  total (157/168)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP0425831,963,1680TA99.4% 758.6 / NA 325intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base A (157/165);  minor base C (0/2);  total (157/168)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 325 total aligned reads displayed.

AGCCCAGCAGTACCAGCAGCACCACCAGCAACAGGATCATAAACAGCTCACGCAGGCTCATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGGCTATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGGCTCA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCGCTCAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACGCTGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAA  >  CP042583/1962892‑1963446
                                                                                                                                                                                                                                                                                   |||                                                                                                                                                                                                                                                                                       
aGCCCAGCAGTACCAGCAGCACCACCAGCAACAGGATCATAAACAGCTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAg                                                                                                                                                                                                                                                                            <  1:818832/289‑1 (MQ=255)
aGCCCAGCAGTACCAGCAGCACCACCAGCAACAGGATCATAAACAGCTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAg                                                                                                                                                                                                                                                                            <  2:2421881/289‑1 (MQ=255)
   ccAGCAGTACCAGCAGCACCACCAGCAACAGGATCATAAACAGCTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCa                                                                                                                                                                                                                                                                         >  1:217050/1‑289 (MQ=255)
           gccAGCAGCACCACCAGCAACAGGATCATAAACAGCTCACGCAGCGACATACCTGGCAGTTCCTGGCGGGCAAGCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCa                                                                                                                                                                                                                                                                    <  2:443697/285‑1 (MQ=255)
           aCCAGCAGCACCACCAGCAACAGGATCATAAACAGCTCACGCAGCGACATCCCTGGCAGTTCCTGGTTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATc                                                                                                                                                                                                                                                                  <  1:1697293/288‑1 (MQ=255)
            ccAGCAGCACCACCAGCAACAGGATCATAAACAGCTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCtt                                                                                                                                                                                                                                                                >  1:2130987/1‑289 (MQ=255)
                   caccacCAGCAACAGGATCATAAACAGCTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGCATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGACAACGCAAAGAACAGCGAGA‑GTCCTGTCAGCCATTTCATCCTGACCcac                                                                                                                                                                                                                                                         >  2:587532/1‑289 (MQ=255)
                    accaccAGCAACAGGATCATAAACAGCTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCcaca                                                                                                                                                                                                                                                        >  2:847238/1‑289 (MQ=255)
                    accaccAGCAACAGGATCATAAACAGCTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCcaca                                                                                                                                                                                                                                                        >  1:32284/1‑289 (MQ=255)
                    accaccAGCAACAGGATCATAAACAGCTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCcaca                                                                                                                                                                                                                                                        >  1:166777/1‑289 (MQ=255)
                    accaccAGCAACAGGATCATAAACAGCTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGACATTTCATCTTGCTCcaca                                                                                                                                                                                                                                                        >  1:2313613/1‑289 (MQ=255)
                                       tAAACAGCTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGATGCCGAACAGAATCATAAATTCGCCGACGAACTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACACCAACC‑GTGCGGACAGCCATTTCATCTTTCCCCACAGCCCCCCCATCAGACGCAt                                                                                                                                                                                                                                     >  2:2145761/1‑289 (MQ=255)
                                          aCAGCTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGCAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATAT‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCTGTGCCACGCATCCCAAGCGTTGCTACCCCAAAGAACAGCGAAA‑GATCTGGCAGCCATTTCAACTTGCTCACCACACTGCCCATCTTGCGCATGTc                                                                                                                                                                                                                                  >  2:1596690/1‑289 (MQ=255)
                                             gCTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTcgcg                                                                                                                                                                                                                               >  1:1905169/1‑289 (MQ=255)
                                              cTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATATCGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCgg                                                                                                                                                                                                                              >  1:1183388/1‑289 (MQ=255)
                                              cTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCa                                                                                                                                                                                                                                                                    <  2:1309762/251‑1 (MQ=255)
                                              cTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCGTCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCgg                                                                                                                                                                                                                              >  1:199137/1‑289 (MQ=255)
                                              cTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCgg                                                                                                                                                                                                                              >  1:1602762/1‑289 (MQ=255)
                                              cTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCgg                                                                                                                                                                                                                              >  2:1368238/1‑289 (MQ=255)
                                              cTCACGCAGCGACATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGCCGCgg                                                                                                                                                                                                                              >  2:666823/1‑289 (MQ=255)
                                               ccacgccgcgacATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATGGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTcg                                                                                                                                                                                                                                 <  1:2698497/274‑1 (MQ=255)
                                                        gacATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGa                                                                                                                                                                                                                       <  2:795350/284‑1 (MQ=255)
                                                        gacATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATa                                                                                                                                                                                                                     <  1:666823/286‑1 (MQ=255)
                                                        gacATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATAc                                                                                                                                                                                                                    <  1:894645/287‑1 (MQ=255)
                                                             cccTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCa                                                                                                                                                                                                               >  1:983333/1‑289 (MQ=255)
                                                             cccTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCa                                                                                                                                                                                                               <  1:2206335/289‑1 (MQ=255)
                                                             cccTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCa                                                                                                                                                                                                               >  1:1251920/1‑289 (MQ=255)
                                                                       tCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATgcg                                                                                                                                                                                                                                        <  2:2727223/254‑1 (MQ=255)
                                                                       tCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATgcg                                                                                                                                                                                                                                        >  1:2727223/1‑254 (MQ=255)
                                                                       tCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTcgc                                                                                                                                                                                                                                <  2:2119179/262‑1 (MQ=255)
                                                                          tgggtggcATTGTGGCTTTCCGCTTTACCGAAGTAAGCGCGATGTAACATGGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATATCGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACcac                                                                                                                                                                                                  <  2:1183388/289‑1 (MQ=255)
                                                                          gggttggcAATCGGGCTTTTCACTTTCCCGAAGGAAGCGCGATGTAACATCGCACGCGAATAAACAGATGCAAAGACCAGACCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACcac                                                                                                                                                                                                  <  2:2664846/285‑1 (MQ=255)
                                                                             ctggcAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCACGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACaa                                                                                                                                                                                                <  1:2738645/288‑1 (MQ=255)
                                                                                gcAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCa                                                                                                                                                                                                                                                                    <  1:759206/217‑1 (MQ=255)
                                                                                gcAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGaa                                                                                                                                                                                            >  1:523805/1‑289 (MQ=255)
                                                                                            ttCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATCCGTTCCTAAAGCTTACCACAAAGAATAAACAGAccc                                                                                                                                                                                 >  2:2195955/1‑288 (MQ=255)
                                                                                                  ttACCGAAGTAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTcgc                                                                                                                                                                                                                                >  2:755871/1‑235 (MQ=255)
                                                                                                    aCCGAAGTAAGCGCGATGTAAAATCGCCAGCGAAAAAACAGATGAAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCcgccgccgac                                                                                                                                                                        <  2:32284/289‑5 (MQ=255)
                                                                                                           tAAGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCcgccgccgacaaac                                                                                                                                                                    >  2:168813/1‑278 (MQ=255)
                                                                                                             aGCGCGATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGACGCTGCCGAACAGAATCATAAATCCGCCGACGAAGTTACCGGTGCCAGGCATCCCAATCGTTGCCCCCGCCAAGAACAGCGACA‑GTGCTGGCAGCCATTTCCTCTTTCTCAACACACCGCCCATCAAGCGCATGTCCCGGGTATGGATACCGTCATCAAGCTCACCACAAAGAAAAAAGAGACACGCCACACACAAAcccggc                                                                                                                                                               >  2:2079770/1‑285 (MQ=255)
                                                                                                               cgcgATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATgcg                                                                                                                                                                                                                                        >  2:1715974/1‑214 (MQ=255)
                                                                                                               cgcgATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATgcg                                                                                                                                                                                                                                        <  1:1715974/214‑1 (MQ=255)
                                                                                                               cgcgATGTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTgcgc                                                                                                                                                             >  1:49310/1‑289 (MQ=255)
                                                                                                                     gTAACATCGCCAGCGAATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCCTGCCCAaaca                                                                                                                                                        >  2:2373765/1‑288 (MQ=255)
                                                                                                                              ccagcgaataAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCACCCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCg                                                                                                                                                                                                                                                   <  1:2079903/182‑1 (MQ=255)
                                                                                                                              ccagcgaataAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCa                                                                                                                                                                                                                                                                    >  1:1514139/8‑171 (MQ=255)
                                                                                                                              ccagcgaataAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACcac                                                                                                                                                                                                  >  1:1436192/8‑237 (MQ=255)
                                                                                                                              ccagcgaataAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTggctgt                                                                                                                                               >  1:1106756/8‑284 (MQ=255)
                                                                                                                                  cgaataAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATgcg                                                                                                                                                                                                                                        <  2:1281305/192‑1 (MQ=255)
                                                                                                                                  cgaataAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACcgc                                                                                                                                           >  1:2254021/4‑288 (MQ=255)
                                                                                                                                   gaataAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACcg                                                                                                                                            <  1:1636510/284‑1 (MQ=255)
                                                                                                                                   gaataAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACcg                                                                                                                                            <  2:629843/284‑1 (MQ=255)
                                                                                                                                   gaataAACAGAGACAAAGACAAGCCCAAAGGTAGAGCAAACGGTAAGCA‑CCGGTACAGCCTGGAAGCTGCCGAACGGAAGCATAAAGTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCCACTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACcgc                                                                                                                                           <  2:2684959/285‑1 (MQ=255)
                                                                                                                                        aaCAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCAACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTgg                                                                                                                                    <  2:1476039/289‑1 (MQ=255)
                                                                                                                                                      ccAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAATCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAGCCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGc                                                                                                                       <  2:709957/288‑1 (MQ=255)
                                                                                                                                                       cAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCCTGCGCATGTCGCGGGTATGGATACGGTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCCGCATCATCTGGCATACCCCGCCCTGGTACGCCAAccgcccc                                                                                                                     >  2:2024550/1‑283 (MQ=255)
                                                                                                                                                       cAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTg                                                                                                                     <  1:2304400/289‑1 (MQ=255)
                                                                                                                                                        aGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTg                                                                                                                     >  1:1378960/1‑288 (MQ=255)
                                                                                                                                                         gCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGt                                                                                                                                                                                                                            >  1:2032116/1‑184 (MQ=255)
                                                                                                                                                         gCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACcac                                                                                                                                                                                                  <  2:2001270/210‑1 (MQ=255)
                                                                                                                                                         gCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAatc                                                                                                                                                         <  1:2447000/251‑1 (MQ=255)
                                                                                                                                                           ccAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCg                                                                                                                  <  1:71941/288‑1 (MQ=255)
                                                                                                                                                            cacaggtgatagaaaCGGTAATCA‑CCGGTACAGCCGGGTAGGATCCGAACAGGACAATAAATTCAACCACGAAGTGACCGGGGCCAGGCATCCCAAGCGTTGTCACCGCCAAGAACAGCGCCA‑GTGCTGGAAGCCAGTTCATCTTGCGCCACAGACCGCCCATCATGCGAATGTGGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGGGGGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAGCTGGCTGCCTgt                                                                                                                <  2:466298/276‑1 (MQ=255)
                                                                                                                                                            cAAAGGAAGAGATAAAGGTAATCA‑CCGTTACAACCTGTAAGCTGCCGAACAGAAGCAAAAATTCGCCGACGAAGTTTCCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAGGAACAGCTACA‑TTGCTGGCAGCCATTTCGTCGTGCTCCACATACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCGATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGgt                                                                                                                <  2:2332994/289‑1 (MQ=255)
                                                                                                                                                                   agagATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATa                                                                                                         <  1:1328587/289‑1 (MQ=255)
                                                                                                                                                                    gagaTAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTAc                                                                                                                                              <  1:1078570/251‑1 (MQ=255)
                                                                                                                                                                          aCGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACaa                                                                                                                                                                                                >  2:2321999/1‑195 (MQ=255)
                                                                                                                                                                           cGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACaa                                                                                                                                                                                                <  1:1038536/194‑1 (MQ=255)
                                                                                                                                                                                aTCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCAGGTAGGCACACTGGACTCCGGTGTAGATAGCCATCAGCCCGa                                                                                            >  2:2044915/1‑289 (MQ=255)
                                                                                                                                                                                 tCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTGGCAACCGCAAAAAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGGATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGaa                                                                                           <  2:1551841/289‑1 (MQ=255)
                                                                                                                                                                                      cGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGTTAGCAATCAGCACGAAGcc                                                                                        >  2:1439342/1‑288 (MQ=255)
                                                                                                                                                                                                tGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGaa                                                                              >  1:1555068/1‑288 (MQ=255)
                                                                                                                                                                                                        gCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCTTGCGCAATCCTCTGGATTACAGCGCCCTGGTAGGCCAACTGGCTGCCGGGGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAAccgaggtg                                                                     >  2:546024/1‑282 (MQ=255)
                                                                                                                                                                                                                  aTCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCg                                                               <  1:2009159/285‑1 (MQ=255)
                                                                                                                                                                                                                   tCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCCTCCCAACCTTTGCCACCGCAAAGAACAGCGACA‑GTACTGGCAGCCATTTCATCTTGATCCACAGACCGCCCATCATGCGCATGGCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAg                                                          <  1:2627597/289‑1 (MQ=255)
                                                                                                                                                                                                                   tCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAg                                                          <  1:256011/289‑1 (MQ=255)
                                                                                                                                                                                                                               cgacgaAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACcaca                                                                                                                                                                                                 >  2:1913554/1‑142 (MQ=255)
                                                                                                                                                                                                                                  cgaAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGt                                           <  1:847238/289‑1 (MQ=255)
                                                                                                                                                                                                                                   gaAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTc                                          <  1:1453506/289‑1 (MQ=255)
                                                                                                                                                                                                                                       ttACCGGGGCCGGGCATCCCAAGCGTTGCCACCGCAAAGCACAGCCACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTggg                                      <  2:2254021/289‑1 (MQ=255)
                                                                                                                                                                                                                                         aCCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTTGGCg                                    <  2:1404497/289‑1 (MQ=255)
                                                                                                                                                                                                                                          gaataagagacagccccAACCGTTGCAACCGCAAAGAACAGGGACA‑GTGCAGGCAGCCATTTCTTCTTGCTCCGCAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCg                                    <  2:938264/274‑1 (MQ=255)
                                                                                                                                                                                                                                              tGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACg                                                                                             >  2:1063304/1‑227 (MQ=255)
                                                                                                                                                                                                                                                    gCATCCCAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATgcg                                                                                                                                                                                                                                        <  1:23684/82‑1 (MQ=255)
                                                                                                                                                                                                                                                        cccAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAAccg                                                                          <  1:1225016/236‑3 (MQ=255)
                                                                                                                                                                                                                                                        cccAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAAccg                                                                          >  2:1225016/1‑234 (MQ=255)
                                                                                                                                                                                                                                                        cccAAGCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAg                                 >  1:1291901/1‑277 (MQ=255)
                                                                                                                                                                                                                                                        cccAAGCGTTGCCACCGCAAAGAACAGCGACA‑GGGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCa                     <  1:704026/289‑1 (MQ=255)
                                                                                                                                                                                                                                                        cccAAACGTTCACACCGCAAAGAACACAGACA‑TTTCTGGCAGCCATTTTACCTTGGTCCACAGACCCGCCAACAATGGCATGGTGCTGGTATTGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCACCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCAAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCa                     <  2:1094021/289‑1 (MQ=255)
                                                                                                                                                                                                                                                             gCGTTGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACg                       >  2:1525068/1‑282 (MQ=255)
                                                                                                                                                                                                                                                               gtgtccACCGCAAAGATCA‑GCTAACGTGCTAGCAGCCATGTCATCTTGTTCCACAGACCGCCCATCATGCGCATGTAGCTGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTag               <  2:1251920/284‑1 (MQ=255)
                                                                                                                                                                                                                                                                 tGCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACccgccg                                                                                                                                                                             <  1:2324406/128‑1 (MQ=255)
                                                                                                                                                                                                                                                                  gCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTg                                                                                                                     <  1:2668397/183‑1 (MQ=255)
                                                                                                                                                                                                                                                                  gCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTagaaga           >  1:1811682/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                  gCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTagaaga           >  1:456003/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                  gCCACCGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGACCAGACGCTTGAACTCGGCCTGGGCGAAGGTCATCCACGCACCGCagaata           >  2:1156750/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                       cGCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGtt                                                     <  2:1807593/242‑1 (MQ=255)
                                                                                                                                                                                                                                                                        gCAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGCCCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCACTCCTCTGGACTACCGCGCCCTCGCAGGCCAACTGGCTGCCGGTGTAGATGGCAACCAGCACGCAGCCCATGTGGGAAACCGACGTGTAGCCGATCCGACGCTCGATATCGGCCTGGGCGAAGGCCTCCAACGCACCGTagaatagaccta     >  2:2502472/1‑281 (MQ=255)
                                                                                                                                                                                                                                                                         cAAAGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGAt    <  1:585349/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                           aaGAACAGCGACA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCCGACAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATaa  >  2:1654137/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                   |||                                                                                                                                                                                                                                                                                       
AGCCCAGCAGTACCAGCAGCACCACCAGCAACAGGATCATAAACAGCTCACGCAGGCTCATCCCTGGCAGTTCCTGGCTGGCAATCTGGCTTTTCGCTTTACCGAAGTAAGCGCGATGTAACATCGCCAGGCTATAAACAGATGCAAAGACCAGCCCAAAGGTAGAGATAACGGTAATCA‑CCGGTACAACCTGGAAGCTGCCGAACAGAATCATAAATTCGCCGACGAAGTTACCGGTGCCAGGCATCCCAAGCGTTGCCACCGCAAAGAACAGGCTCA‑GTGCTGGCAGCCATTTCATCTTGCTCCACAGACCGCCCATCATGCGCATGTCGCGGGTATGGATACGTTCATAAAGCTGACCACAAAGAATAAACAGACCCGCCGCGCTCAAACCGTGCGCAATCATCTGGATTACCGCGCCCTGGTAGGCCAACTGGCTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACGCTGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAGGCCATCCACGCACCGTAGAAGATGCCGATAA  >  CP042583/1962892‑1963446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A