Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,967,511 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,967,5110GC99.1% 745.6 / NA 318intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base C (153/162);  total (153/165)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP0425831,967,5120CG99.4% 736.4 / NA 318intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base G (150/164);  minor base T (1/1);  total (153/165)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP0425831,967,5130TA98.4% 724.7 / NA 318intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (149/160);  minor base G (1/4);  total (153/165)
Fisher's exact test for biased strand distribution p-value = 3.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 318 total aligned reads displayed.

TTCGTTCTCTGCTTCGCCCTTATCTTTGCCGTCGATTGCCATACCTGCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGACTGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGGCTGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGC  >  CP042583/1967232‑1967792
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                       
         tGCTTCGCCCTTATCTTTGCCGTCGATTGCCATACCTGCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCggg                                                                                                                                                                                                                                                                         <  2:145721/289‑1 (MQ=255)
                 ccTTATCTTTGCCGTCGATTGCCATACCTGCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTa                                                                                                                                                                                                                                                                  <  1:2698605/288‑1 (MQ=255)
                   ttATCTTTGCCGTCGATTGCCATACCTGCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCAAGATACATCTGACCGGGCAGGCTAcc                                                                                                                                                                                                                                                                >  2:1330861/1‑288 (MQ=255)
                     aTCTTTGCCGTCGATTGCCATACCTGCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCgg                                                                                                                                                                                                                                                                          >  2:1795990/1‑276 (MQ=255)
                        tttGCCGTCGATTGCCATACCTGCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCag                                                                                                                                                                                                                                                          <  2:2266092/289‑1 (MQ=255)
                        tttGCCGTCGATTGCCATACCTGCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCag                                                                                                                                                                                                                                                          <  2:355655/289‑1 (MQ=255)
                        tttGCCGTCGATTGCCATACCTGCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGAGCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAAACGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCag                                                                                                                                                                                                                                                          <  1:1431400/289‑1 (MQ=255)
                          tGCCGTCGATTGCCATACCTGCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCACCCATTTCGACATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTGGGTTTCTGCTTTTTGCAGCGAGATACAGCCGAACGGGCCGGCTCACGCGCaggg                                                                                                                                                                                                                                                        >  2:763286/1‑287 (MQ=255)
                           gCCGTCGATTGCCATACCTGCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCATTGGTTTCTGCTTTTTTCAGCGAGATACAGCCGACCGGGCCGGCTACCGCGAACAgg                                                                                                                                                                                                                                                       >  2:1110057/1‑289 (MQ=255)
                             cGTCGATTGCCATACCTGCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGtt                                                                                                                                                                                                                                                     <  2:1275791/289‑1 (MQ=255)
                             cGTCGATTGCCATACCTGACCTCCGGTAAGAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCAGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGtt                                                                                                                                                                                                                                                     <  2:199507/289‑1 (MQ=255)
                               tCGATTGCCATACCTGCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGCTACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTAc                                                                                                                                                                                                                                                   >  2:801969/1‑289 (MQ=255)
                                         tACCTGCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCa                                                                                                                                                                                                                                         <  2:2486820/289‑1 (MQ=255)
                                          aCCTGCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCaa                                                                                                                                                                                                                                        <  2:1504257/289‑1 (MQ=255)
                                           ccTGCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAAc                                                                                                                                                                                                                                       <  1:1713421/289‑1 (MQ=255)
                                           ccTGCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAAc                                                                                                                                                                                                                                       <  1:1145171/289‑1 (MQ=255)
                                              gCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGc                                                                                                                                                                                                                                     >  2:1207797/1‑288 (MQ=255)
                                                      gTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCg                                                                                                                                                                                                                            >  1:404666/1‑289 (MQ=255)
                                                               atatTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTTAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTcg                                                                                                                                                                                                                   <  1:2155329/289‑1 (MQ=255)
                                                               atatTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAgg                                                                                                                                                                                                                                                >  2:337053/1‑260 (MQ=255)
                                                               atatTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTc                                                                                                                                                                                                                    >  1:1254615/1‑288 (MQ=255)
                                                                    cccggTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGt                                                                                                                                                                                                              <  1:886124/286‑1 (MQ=255)
                                                                       ggTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCggg                                                                                                                                                                                                                                                                         >  2:469682/1‑227 (MQ=255)
                                                                       ggTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGt                                                                                                                                                                                                              <  1:2535108/286‑1 (MQ=255)
                                                                       ggTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAg                                                                                                                                                                                                           >  2:217139/1‑289 (MQ=255)
                                                                        ggaTTTGCCCGGACCGTAGGTCAGCCGATCCTCTTTATCGTCAGCCAGGTCCTGGCGTTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGGGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGGGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAg                                                                                                                                                                                                           <  2:1449702/286‑1 (MQ=255)
                                                                        gTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAg                                                                                                                                                                                                           <  1:302873/288‑1 (MQ=255)
                                                                        gTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAg                                                                                                                                                                                                           <  1:335828/288‑1 (MQ=255)
                                                                        gTATTTGCCCGGACCGGAGATCAGCAGATCCCCTTTCTCGTAAACCTGATCCGTGCGCTTGTATTCCCCCATTTCTAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAg                                                                                                                                                                                                           <  2:643898/288‑1 (MQ=255)
                                                                            ttGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGCGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGa                                                                                                                                                                                                      >  1:2731466/1‑289 (MQ=255)
                                                                                  ggaccggaGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGAc                                                                                                                                                                                                <  1:663336/289‑1 (MQ=255)
                                                                                   gaccggaGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACCCCCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACgcc                                                                                                                                                                                                >  2:789082/1‑286 (MQ=255)
                                                                                            acagcagATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACg                                                                                                                                                                                      <  2:2534531/288‑1 (MQ=255)
                                                                                              agcagATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCa                                                                                                                                                                                                                                         >  1:470516/1‑236 (MQ=255)
                                                                                              agcagATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACggg                                                                                                                                                                                    >  2:2070664/1‑289 (MQ=255)
                                                                                              agcagATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACggg                                                                                                                                                                                    >  1:240217/1‑289 (MQ=255)
                                                                                              agcagATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCAGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCag                                                                                                                                                                                                                                                          >  1:2017431/1‑219 (MQ=255)
                                                                                                cagATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTcg                                                                                                                                                                                                                   >  2:1864556/1‑256 (MQ=255)
                                                                                                 agATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGggc                                                                                                                                                                                  <  2:1750366/288‑1 (MQ=255)
                                                                                                   aTCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCCCTCTGCCGTCCGGGTCGCGGGTCCGAACGATACGACCACGCTAAAGGgggggc                                                                                                                                                                               >  2:71013/1‑289 (MQ=255)
                                                                                                    tCCTCTTTCACGGAAACCAGACCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTTGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGggcg                                                                                                                                                                                 <  1:1739388/286‑1 (MQ=255)
                                                                                                     ccTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAg                                                                                                                                                                             >  1:1871891/1‑289 (MQ=255)
                                                                                                        ctTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGAt                                                                                                                                                                                                     <  2:2125004/262‑1 (MQ=255)
                                                                                                        ctTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGAt                                                                                                                                                                                                     >  1:2125004/1‑262 (MQ=255)
                                                                                                          ttCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAg                                                                                                                                                                         >  1:2361908/1‑288 (MQ=255)
                                                                                                                tAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAg                                                                                                                                                                                                                                                 <  2:595723/210‑1 (MQ=255)
                                                                                                                tAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAg                                                                                                                                                                                                                                                 >  1:595723/1‑210 (MQ=255)
                                                                                                                  aaCCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGctct                                                                                                                                                                >  1:1548365/1‑289 (MQ=255)
                                                                                                                                   tGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACAttcgcgt                                                                                                                                               >  1:56223/1‑289 (MQ=255)
                                                                                                                                         cccccATTTCGAAGTCCGGGGTTAACTGAGTCGCCGTGGTCGGCCAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACAttcgcgtttcgcg                                                                                                                                         <  2:2441697/289‑1 (MQ=255)
                                                                                                                                          ccccATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCAGGGTACAttcgcgtttcgcgt                                                                                                                                        >  1:1528000/1‑289 (MQ=255)
                                                                                                                                               tttCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCag                                                                                                                                                                                                                                                          >  1:2468593/1‑170 (MQ=255)
                                                                                                                                               tttCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGc                                                                                                                                   >  1:1835653/1‑289 (MQ=255)
                                                                                                                                               tttCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGc                                                                                                                                   >  1:97508/1‑289 (MQ=255)
                                                                                                                                                            ggTTAGCGGAATCACGGTGGTCGGAGAGGATTCTACGAAAGTGCCCCAGAAAAGTCAGCGTGGGAAGTTGAGGCGGAGAAATTCCGGGTACCAGCGTCCGTCTTTGGTTTCTGCTTGTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCGGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAg                                                                                                                       <  2:2422726/288‑1 (MQ=255)
                                                                                                                                                               tgccTGAATCGCCGTGTGCCGACAGGCTGCTTCGCACAGACCACAGAACATGGAGCATTAGAAGTTCATGCGGAAAAAGTCCGGGTACCAGAGACCGTCTTTGGTTGCTGCTTATTGCGGGGAGATACAGCCAGCCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCGCTTCGCCGTCCTGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGAGAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCg                                                                                                                   <  2:268965/286‑1 (MQ=255)
                                                                                                                                                               tAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCg                                                                                                                   <  1:2070664/289‑1 (MQ=255)
                                                                                                                                                               tAACGGAATCGCCGGGGTCGGACAAGCTTCGTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCg                                                                                                                   <  2:56223/289‑1 (MQ=255)
                                                                                                                                                                 agtgatccgacGTGGTCGGACAGGCTTCTGCGCACAGGCCACAGGAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCGCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGat                                                                                                                 <  2:1544433/279‑1 (MQ=255)
                                                                                                                                                                        cGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCg                                                                                                                   >  1:1205580/1‑280 (MQ=255)
                                                                                                                                                                          ccGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCag                                                                                                                                                                                                                                                          <  1:1303852/143‑1 (MQ=255)
                                                                                                                                                                                    aCAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGTGAACGCGTGCAGGCCGATCATCCAGa                                                                                                         <  1:1090637/278‑1 (MQ=255)
                                                                                                                                                                                    aCAGGCTTCTTCGCAACGACCACAGAAAATGCAGCGGGGGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAAc                                                                                               <  2:2296875/288‑1 (MQ=255)
                                                                                                                                                                                         cttcttCGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGggc                                                                                                                                                                                  <  2:2356793/200‑1 (MQ=255)
                                                                                                                                                                                            cttcGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCa                                                                                                                                                                                              <  1:1001805/185‑1 (MQ=255)
                                                                                                                                                                                              tcGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGa                                                                                    <  1:217139/289‑1 (MQ=255)
                                                                                                                                                                                              tcGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGa                                                                                    <  2:2200852/289‑1 (MQ=255)
                                                                                                                                                                                              tcGCACAGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGa                                                                                    <  2:240217/289‑1 (MQ=255)
                                                                                                                                                                                              tcGCACAGAACACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCAGGGGACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGCTACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGa                                                                                    <  1:1190183/289‑1 (MQ=255)
                                                                                                                                                                                                  acaGACCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCGGATACTACTAACCTGGGTGCCGAAAcc                                                                                >  2:1680537/1‑289 (MQ=255)
                                                                                                                                                                                                      aCCACAGAAAATGCAGCGTGAGAAGTTGATGCGGCAACATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCCACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATCACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTTGCGAACGCCTGCAGGCCGATCATCCAGATACTAAGAAGCTGGTTGCCGAAACctccg                                                                            >  2:40374/1‑286 (MQ=255)
                                                                                                                                                                                                      aCCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATa                                                                                                                                                                                                    >  1:1761164/1‑169 (MQ=255)
                                                                                                                                                                                                      aCCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAAc                                                                                               <  1:220057/270‑1 (MQ=255)
                                                                                                                                                                                                      aCCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACctact                                                                            >  1:851275/1‑289 (MQ=255)
                                                                                                                                                                                                      aCCACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCAAAACctact                                                                            >  1:684881/1‑289 (MQ=255)
                                                                                                                                                                                                      aCCAAAGAAAATGCAGCGTGAGCAGTTGATGTGGAGAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGGGATACAGCCGACCGGGCAGGCTACCGCTCAGAGGATACAGGCTACGCAACGCACTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGTCACCGAAAACGGGGCGGCAGGTAAACGGGCTGTTCCTGGTACATTCGCGGTTCGCCTTTGGCGAACGCGTGCAGGGCGGTCATGCAGATACTATGAACCTGGGTGCCGAAACcaaca                                                                            >  2:321235/1‑288 (MQ=255)
                                                                                                                                                                                                       ccACAGAAAATGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACctacta                                                                           >  2:1651169/1‑289 (MQ=255)
                                                                                                                                                                                                              aaaTGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACaa                                                                       <  2:1197155/286‑1 (MQ=255)
                                                                                                                                                                                                              aaaTGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTGTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACaa                                                                       <  1:747753/286‑1 (MQ=255)
                                                                                                                                                                                                                aTGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGc                                                                                                                                                                                                                                     >  1:1350942/1‑126 (MQ=255)
                                                                                                                                                                                                                aTGCAGCGTGAGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCtt                                                                  >  1:2005940/1‑289 (MQ=255)
                                                                                                                                                                                                                  gcagcgtgagAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGACAACGGGGCGGCAGATACACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTTGCGAACGCGTGCAGGCTGCTCATCCAGATACGACGCACCTGGGTGCCGACACCTACTAACAATCAtttt                                                                >  2:870778/10‑289 (MQ=255)
                                                                                                                                                                                                                    agcgtgagAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTa                                                               <  1:1622368/281‑1 (MQ=255)
                                                                                                                                                                                                                    agcgtaagAAGTTGATGCGTAAAAATTCCGGGTACCAGCGCCCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTa                                                               <  1:2281212/281‑1 (MQ=255)
                                                                                                                                                                                                                     gcgtgagAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAg                                                             >  2:1075222/7‑289 (MQ=255)
                                                                                                                                                                                                                      cgtgagAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACAttcgcgttt                                                                                                                                             >  2:84929/6‑208 (MQ=255)
                                                                                                                                                                                                                         gagAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCa                                                         <  1:1400921/287‑1 (MQ=255)
                                                                                                                                                                                                                         gagAAGCTGATGCGGACAAATTCCGGGTACAAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGGGATACAGCCGACCGCGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGACCGATACGACCACGATACCGGGGTGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCa                                                         <  1:2256065/287‑1 (MQ=255)
                                                                                                                                                                                                                          agAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCa                                                         <  1:36918/287‑1 (MQ=255)
                                                                                                                                                                                                                                  aTGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCg                                                                                                                   >  2:1406167/1‑222 (MQ=255)
                                                                                                                                                                                                                                                cGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCAGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTgcg                                  <  1:2356511/289‑1 (MQ=255)
                                                                                                                                                                                                                                                cGGGGACCAGCGACCGGCTTTGGTTTCTGCTTTTGGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTgcg                                  <  2:1373053/289‑1 (MQ=255)
                                                                                                                                                                                                                                                     aCCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGc                             <  1:801969/289‑1 (MQ=255)
                                                                                                                                                                                                                                                              cgccTTTGGTTTCTGCTTTGTGCAGCGAGATACAGCGGACGGGGCAGGCTACCGCGCGGAGGTCACAGGCTACGCAACGCTCTTCGCCGTCCGTGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATg                    <  2:1594500/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                  tttGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGat                                                                                                                 >  1:454006/1‑192 (MQ=255)
                                                                                                                                                                                                                                                                  tttGGTTTCTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGat                                                                                                                 <  2:454006/192‑1 (MQ=255)
                                                                                                                                                                                                                                                                          cTGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTTCGCCTGCCAGAGAATGCCAGCCGCCCtt        >  2:1159498/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                           tGCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTa                                                               <  2:2622566/233‑1 (MQ=255)
                                                                                                                                                                                                                                                                            gCTTTTTGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGCTCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGGTTTATTTGCCCTTATTTCCCCCTTCCGAGAATTACAGCCCGCGTTa       >  2:2715442/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                              tttttGCAGCGAGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCa    <  1:1789245/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                       
TTCGTTCTCTGCTTCGCCCTTATCTTTGCCGTCGATTGCCATACCTGCCATCCGGTAGAAGTTATATTCCGGGTATTTGCCCGGACCGGAGATCAGCAGATCCTCTTTCTCGTAAACCAGATCCTGGCGCTTGTATTCCCCCATTTCGAAATCCGGGGTTAACTGAATCGCCGTGGTCGGACAGGCTTCTTCGCACAGACCACAGAAAATGCAGCGACTGAAGTTGATGCGGAAAAATTCCGGGTACCAGCGACCGTCTTTGGTTTCTGCTTTTTGCAGGCTGATACAGCCGACCGGGCAGGCTACCGCGCAGAGGTTACAGGCTACGCAACGCTCTTCGCCGTCCGGGTCGCGGGTCAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGC  >  CP042583/1967232‑1967792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A