Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,968,452 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,968,4520GC99.7% 724.0 / NA 310intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (155/153);  total (156/154)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,968,4530CG98.7% 712.6 / NA 310intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base G (152/153);  total (156/154)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,968,4540TA99.4% 723.4 / NA 310intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (155/152);  minor base G (0/2);  total (156/154)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 310 total aligned reads displayed.

CACATGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAAACTACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAAGCTGTATTTGTTGTT‑ACTACTCCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGGCTGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCGCTGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGGCTACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGACTTATCCAACTCAT  >  CP042583/1968173‑1968730
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                    
cacaTGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAg                                                                                                                                                                                                                                                                               >  1:1959599/1‑289 (MQ=255)
   aTGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCa                                                                                                                                                                                                                                                                            >  1:769381/1‑289 (MQ=255)
     gcgcCTGGCTGTTGACTGTGTCGGTACTGTTGAGTGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCaa                                                                                                                                                                                                                                                                           <  2:2118362/288‑1 (MQ=255)
     gcgcCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCaa                                                                                                                                                                                                                                                                           >  1:2206808/1‑288 (MQ=255)
     gcgcCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAat                                                                                                                                                                                                                                                                          >  2:1127630/1‑289 (MQ=255)
      cgcCTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAatc                                                                                                                                                                                                                                                                         >  2:2198715/1‑289 (MQ=255)
        ccTGGCTGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAatcat                                                                                                                                                                                                                                                                       <  1:424038/289‑1 (MQ=255)
              tGTTGACGCTGTCGGACATGTGGAATGAACCGGCCTGGGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGc                                                                                                                                                                                                                                                                 <  2:844037/289‑1 (MQ=255)
              tGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGc                                                                                                                                                                                                                                                                 <  1:2378793/289‑1 (MQ=255)
              tGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGc                                                                                                                                                                                                                                                                 <  1:161420/289‑1 (MQ=255)
              tGTTGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGc                                                                                                                                                                                                                                                                 <  1:1145818/289‑1 (MQ=255)
                 tGACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAg                                                                                                                                                                                                                                                              >  1:663272/1‑289 (MQ=255)
                  gACGATGTCGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGACCAAAATCCCGCAGTTCAGGTCGGCAACCACCCACCCCGGACTGACTGGCACAATCGCAACGGCCAGCAGCAGCCAGTTACAGGCAAACATCgggcacatg                                                                                                                                                                                                                                                             >  2:650191/1‑282 (MQ=255)
                          cGGTCATGTTGAATGAACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCAAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCGGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAg                                                                                                                                                                                                                                                       >  1:1445588/1‑287 (MQ=255)
                                      atgaaCCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATccga                                                                                                                                                                                                                                         >  1:400186/5‑286 (MQ=255)
                                       tgaaCCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATACCGATGTTCAGGTCGGCAACCACCCAACCCGGACAGACTAGCACAATCGCAAAGGACAGCAGCAGCGATGTAAAGGAAACCATCGGAGCCAGGGGAAAGAGTGCGCGATccgag                                                                                                                                                                                                                                        >  2:1542043/4‑285 (MQ=255)
                                       tgaaCCCGCCTGCGCACCCGCGCCAATCAAGGCAAGCCCGGGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATccgag                                                                                                                                                                                                                                        <  2:2375250/286‑5 (MQ=255)
                                             ggCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTc                                                                                                                                                                                                                                  <  1:1472597/289‑1 (MQ=255)
                                               ccTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCg                                                                                                                                                                                                                                 <  1:1127630/288‑1 (MQ=255)
                                               ccTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCg                                                                                                                                                                                                                                 <  1:1843410/288‑1 (MQ=255)
                                                        ccacGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCGAAGGCCAGCAGCAGCGCGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTTCGGATCCAGt                                                                                                                                                                                                                        >  2:782332/1‑288 (MQ=255)
                                                             cccATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAAGTTCGGGATCCAGTcttctt                                                                                                                                                                                                                  >  1:2027561/1‑289 (MQ=255)
                                                             cccATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACACGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCCAGGCAAAGGCAATCATCGGTGCCAGGGTAAAGATGCACCGATCCGAGAAGTTCGGGATACAggttcctt                                                                                                                                                                                                                  >  2:1504220/1‑282 (MQ=255)
                                                                  cAAGGAAAGCCCGGGGAACAATTCGTAGCTCAGGGTCTGCGCAGACGCACGAATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAg                                                                                                                                                                                                             <  2:1307599/289‑1 (MQ=255)
                                                                  cAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAACTTCGGGATCCAGTCTTCTTTAAAg                                                                                                                                                                                                             >  1:1821801/1‑289 (MQ=255)
                                                                          gCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGcc                                                                                                                                                                                                                                                                <  1:2116556/230‑1 (MQ=255)
                                                                          gCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGa                                                                                                                                                                                                            >  1:1261932/1‑282 (MQ=255)
                                                                          gCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGa                                                                                                                                                                                                            <  2:1261932/282‑1 (MQ=255)
                                                                           cccGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACAtt                                                                                                                                                                                                       <  2:1688283/286‑1 (MQ=255)
                                                                                     acTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCg                                                                                                                                                                                          >  2:1808035/1‑289 (MQ=255)
                                                                                       ttCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCa                                                                                                                                                                                        <  2:222263/289‑1 (MQ=255)
                                                                                         cGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGc                                                                                                                                                                                                                                                                 <  1:1794100/214‑1 (MQ=255)
                                                                                         cGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGc                                                                                                                                                                                                                                                                 >  2:1794100/1‑214 (MQ=255)
                                                                                             gccaaGGGTCCGCGCCGCAGCACGCAGCCCACACAGCAACGAGTGTTTGTTGTTAACTA‑ACCAGACCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGGTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAg                                                                                                                                                                                   <  2:1430474/284‑1 (MQ=255)
                                                                                             gCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAg                                                                                                                                                                                   <  1:2360995/288‑1 (MQ=255)
                                                                                                 aGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGAcg                                                                                                                                                                                                                                                 <  1:173973/222‑1 (MQ=255)
                                                                                                   ggTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAgc                                                                                                                                                                            >  1:1547752/1‑288 (MQ=255)
                                                                                                   ggTCTGCGCAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAgc                                                                                                                                                                            >  1:2577679/1‑288 (MQ=255)
                                                                                                        gcgcAGAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGt                                                                                                                                                                                                                        >  2:2547899/1‑240 (MQ=255)
                                                                                                             gAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCa                                                                                                                                                                                                                          >  2:1345588/1‑233 (MQ=255)
                                                                                                             gAAGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCa                                                                                                                                                                                                                          <  1:1345588/233‑1 (MQ=255)
                                                                                                              aaGCACGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCa                                                                                                                                                                 >  1:2220251/1‑289 (MQ=255)
                                                                                                                   cGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTCAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCa                                                                                                                                                             >  1:1907188/1‑288 (MQ=255)
                                                                                                                   cGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGAcgc                                                                                                                                                                                                                                                <  2:1726918/205‑1 (MQ=255)
                                                                                                                   cGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCa                                                                                                                                                             >  1:833537/1‑288 (MQ=255)
                                                                                                                   cGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCa                                                                                                                                                             >  1:946081/1‑288 (MQ=255)
                                                                                                                   cGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCa                                                                                                                                                             >  1:576091/1‑288 (MQ=255)
                                                                                                                   cGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCa                                                                                                                                                             >  1:299011/1‑288 (MQ=255)
                                                                                                                   cGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCa                                                                                                                                                             >  1:2303063/1‑288 (MQ=255)
                                                                                                                   cGCATCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCACCAGCCaa                                                                                                                                                            >  2:1750053/1‑289 (MQ=255)
                                                                                                                      aTCGCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTACGCAAACAGCTGGACGGAACCGCCCCAGccctgt                                                                                                                                                          >  2:1459031/1‑284 (MQ=255)
                                                                                                                         gaaCCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGt                                                                                                                                                      <  1:1433260/287‑1 (MQ=255)
                                                                                                                         gCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCTAGCCAACACGGt                                                                                                                                                      <  1:1808035/289‑1 (MQ=255)
                                                                                                                         gCACCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCATAAAGCGCCAGACCTGCCATCATCAGGATAAACAAAATCCCGATGTTCAGGGCGGCAACCACCCACACCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCAGCGGTGCAAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTTTTCTTTAAAGAACATTTTTATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGt                                                                                                                                                      <  2:91102/289‑1 (MQ=255)
                                                                                                                           aCCCAGCAACGAGTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGtt                                                                                                                                                     <  2:2154879/288‑1 (MQ=255)
                                                                                                                                gcaacgagTATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTcc                                                                                                                                               >  1:1517481/8‑289 (MQ=255)
                                                                                                                                    cgcttATTTGTTGTT‑ACTTGACCAGCCCGCAAACAGCACCGCGTAAACCGCCAGAACTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTaa                                                                                                                                           <  1:1045943/285‑1 (MQ=255)
                                                                                                                                       gTATTTGTGCTT‑ACTGGACCACCCCGCAAACAGCACCACGTAAACCCCCAAACCAGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAAc                                                                                                                                          <  2:2425074/287‑1 (MQ=255)
                                                                                                                                                 gttacttgaccAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGACCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCGGGAAc                                                                                                                               >  1:2261401/10‑289 (MQ=255)
                                                                                                                                                  ttacttgaccAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTc                                                                                                                                                                                            <  2:739578/219‑1 (MQ=255)
                                                                                                                                                            aGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCcagcag                                                                                                                    >  1:2311443/1‑289 (MQ=255)
                                                                                                                                                              cccGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGACACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAgg                                                                                                                   >  2:768302/1‑288 (MQ=255)
                                                                                                                                                                  cAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGcc                                                                                                                                                                    >  2:446553/1‑235 (MQ=255)
                                                                                                                                                                        gCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGcc                                                                                                        >  2:1005981/1‑289 (MQ=255)
                                                                                                                                                                        gCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGc                                                                                                         >  2:1152162/1‑288 (MQ=255)
                                                                                                                                                                         taCCGGGTAAAACCGCAGACCTGCCATCATAAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCAAAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCa                                                                                                       <  2:2303063/288‑1 (MQ=255)
                                                                                                                                                                              cgTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGt                                                                                                                                                      >  1:276085/1‑237 (MQ=255)
                                                                                                                                                                              cgTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGc                                                                                                   >  1:857995/1‑288 (MQ=255)
                                                                                                                                                                                      gCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATg                                                                                          <  2:1090179/289‑1 (MQ=255)
                                                                                                                                                                                               gCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTc                                                                                                                                                                                            >  2:1891958/1‑182 (MQ=255)
                                                                                                                                                                                               gCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTc                                                                                                                                                                                            <  1:1891958/182‑1 (MQ=255)
                                                                                                                                                                                               gCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAgg                                                                                 >  1:495431/1‑289 (MQ=255)
                                                                                                                                                                                                  atcatcAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGa                                                                              <  2:2577679/289‑1 (MQ=255)
                                                                                                                                                                                                      tcaGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAac                                                                          <  1:715019/289‑1 (MQ=255)
                                                                                                                                                                                                          gaagaaCAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGAt                                                                                                                                                                                                  >  2:373190/1‑165 (MQ=255)
                                                                                                                                                                                                               aCAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATg                                                                                              >  1:1461140/1‑260 (MQ=255)
                                                                                                                                                                                                                cAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAgg                                                                >  1:1211484/1‑289 (MQ=255)
                                                                                                                                                                                                                   aaTCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATc                                                             >  1:2472290/1‑289 (MQ=255)
                                                                                                                                                                                                                    aTCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAAc                                                                                                                                          <  1:1719108/211‑1 (MQ=255)
                                                                                                                                                                                                                                 ggTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGcc                                                                                                                                                                    >  2:28509/1‑172 (MQ=255)
                                                                                                                                                                                                                                 ggTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACcagc                                                                   <  2:80936/269‑1 (MQ=255)
                                                                                                                                                                                                                                     ggCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAAc                                                                                                                                          <  1:1650117/194‑1 (MQ=255)
                                                                                                                                                                                                                                     ggCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGaa                                                                                                                                >  1:756069/1‑204 (MQ=255)
                                                                                                                                                                                                                                       cAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGCACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCACCGCAGGTGACAACCACCCGCAGGATCACCACCGCTTTGAGGATgg                                          >  1:1324576/1‑288 (MQ=255)
                                                                                                                                                                                                                                          ccaccCAACCCGGACTGACTGGAACAATCGCAAAGGCAAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTcag                                      <  2:1827687/289‑1 (MQ=255)
                                                                                                                                                                                                                                            accCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTCAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTcagca                                    <  2:1907188/289‑1 (MQ=255)
                                                                                                                                                                                                                                            accCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCg                                                                                                                 <  2:2027521/212‑1 (MQ=255)
                                                                                                                                                                                                                                            accCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAg                                                                                                    >  2:1570517/1‑225 (MQ=255)
                                                                                                                                                                                                                                            accCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTcagca                                    <  1:2432122/289‑1 (MQ=255)
                                                                                                                                                                                                                                                     ggACTGACTGGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCaa                           >  1:2562796/1‑289 (MQ=255)
                                                                                                                                                                                                                                                       actgactgGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTCGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATCCCCCGCAGGTGACAACCACCCGCCGGAGCAACACCGCCTTGGGGGTGGTCAAGAGGAACCCaacc                         >  2:2620731/1‑287 (MQ=255)
                                                                                                                                                                                                                                                           actgGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTaa                                                                                                                                           >  1:371602/1‑171 (MQ=255)
                                                                                                                                                                                                                                                           actgGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGt                      >  1:793386/1‑288 (MQ=255)
                                                                                                                                                                                                                                                           actgGCACAATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCCCTTTGAGGATGGTCAGCAGGAACCCAAGCAGt                      >  2:1242503/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                aacaATACCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATAGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCATTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCgg                 <  2:1950741/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                 acaATCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCggtg               <  1:2180421/289‑3 (MQ=255)
                                                                                                                                                                                                                                                                     tCGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCCCCCCTTTGAGGAGGGTCAGCAGGACCCCAATCAGTTCCggggaca            >  2:2324100/1‑283 (MQ=255)
                                                                                                                                                                                                                                                                      cGCAAAGGCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCggtgatatc          <  2:833537/289‑8 (MQ=255)
                                                                                                                                                                                                                                                                             gCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACcagc                                                                   >  2:326302/1‑225 (MQ=255)
                                                                                                                                                                                                                                                                             gCCAGCAGCAGCGAGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCCGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACcagc                                                                   <  1:326302/225‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                    
CACATGCGCCTGGCTGTTGACGATGTCGGTCATGTTGAAACTACCGGCCTGCGCCACCACGCCCATCAAGGAAAGCCCGAGGAACACTTCGTAGCTCAGGGTCTGCGCAGAAGCACGCATCGCACCCAGCAAGCTGTATTTGTTGTT‑ACTACTCCAGCCCGCAAACAGCACCGCGTAAACCGCCAGACCTGCCATCATCAGGAAGAACAAAATCCCGATGTTCAGGTCGGCAACCACCCAACCCGGACTGACTGGCACAATCGCAAAGGCCAGCAGCAGGCTGGTAAAGGCAATCATCGGTGCCAGGGTAAAGATGACGCGATCGCTGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGGCTACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGACTTATCCAACTCAT  >  CP042583/1968173‑1968730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A