Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F148 I1 R1	14	45.7	1660340	90.3%	1499287	142.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	124.9 / NA	45	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (15/30); total (15/30)

GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGA  >  NC_000913/2132647‑2132919

ggAGCTGGTAGAGGGTAGACAGCTGATATTTTTTGCTGGCGCGGTCGTATTTGTCTTTGTGCTTGCGGTACAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTc                                                                                                                                    <  2:788518/143‑1 (MQ=255)

      ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGCTTGATATGCAGGCTCTGCGTc                                                                                                                              >  1:673226/1‑143 (MQ=255)

      ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGGCGAATTTGTCTCTGTGCTTGCGGTAACAGTGGAAGTACCCGGAGAATTTTTTCGGTGCCGAGGTGACCCGCATTTCCCCCTGACCGATACGCAGGCGCTACGTc                                                                                                                              >  2:259602/1‑143 (MQ=255)

        tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGCCTCTGCGTCGc                                                                                                                            >  2:229033/1‑143 (MQ=255)

            gggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCGGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCcgct                                                                                                                        >  1:453567/1‑143 (MQ=255)

                aagtaGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                    <  2:470000/139‑1 (MQ=255)

                 aaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAc                                                                                                                   >  1:675916/1‑143 (MQ=255)

                     gCTGATATTTTTTGCTGGCGCGGTCGTATTTGTTTTTTTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc                                                                                                               <  1:334804/143‑1 (MQ=255)

                     gCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc                                                                                                               <  1:337412/143‑1 (MQ=255)

                      cTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTcc                                                                                                              >  1:609502/1‑143 (MQ=255)

                       tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                             <  1:99953/143‑1 (MQ=255)

                         atatTTTTGGCTGGAGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACg                                                                                                           <  1:580678/143‑1 (MQ=255)

                          tatTGTTTGCTTGCGCGGTCGAAGTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTGCCCGGAGAATTTGTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACgg                                                                                                          <  2:564011/143‑1 (MQ=255)

                                   cTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa                                                                                                 >  2:52008/1‑143 (MQ=255)

                                     ggCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACt                                                                                               >  1:3847/1‑143 (MQ=255)

                                      gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc                                                                                              <  1:491232/143‑1 (MQ=255)

                                      gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc                                                                                              >  1:755053/1‑143 (MQ=255)

                                      gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc                                                                                              <  1:275176/143‑1 (MQ=255)

                                           gTCGATTTTGTCGTTGGGCTTGCTGTAAAAGTTGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacca                                                                                         <  2:107160/143‑1 (MQ=255)

                                                aTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGGACTCCACCACCATc                                                                                    >  2:549348/1‑143 (MQ=255)

                                                       tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa                                                                             >  1:359994/1‑143 (MQ=255)

                                                        ttggacTTGCGGTAAAAGACGACGTACCCCGAGCATGTTTTCGGCGACGAGGTGATCTGCATTTCACCGTGATTGCTACGCAGGCTCTGCGTCGCCTCTTCTACATTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                            <  1:766200/138‑1 (MQ=255)

                                                         tgtgCTTGTGGTACAAGTGGAAGTACCCGGAGAATTTTGTCGGTGACGAGGTGATCTGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAg                                                                           <  2:517088/143‑1 (MQ=255)

                                                           tgCTTGCGGTAAAAGTGTAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGAt                                                                         <  2:209444/143‑1 (MQ=255)

                                                             ccggCGGTCGAAGTGGAAGTACTCGGATAATGTGTTCGGTGACGAGGTGATCTGCATGTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGt                                                                       <  2:576984/140‑1 (MQ=255)

                                                               tGCGCTAAAAGTCGACGTACCCTGAGAGTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCg                                                                     <  1:340532/143‑1 (MQ=255)

                                                                gCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGt                                                                    >  1:155864/1‑143 (MQ=255)

                                                                    tAAAGGTGGAAGTGCCCGGAGAATTTTTTCGGGGGCGAGGTGATCTGCATTTCACCGTGATTGATAGGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc                                                                <  2:92525/143‑1 (MQ=255)

                                                                          tgccaGTTCCCGGAGCAGTTTTTCGGTGGCGAGGTGTTCTGTATTTCCCCGTGATTGATATGCAGGCTGTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGc                                                          <  2:234736/139‑1 (MQ=255)

                                                                                tACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                    <  1:352329/143‑1 (MQ=255)

                                                                                 aCCCGGAGACTGTGTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTc                                                   <  1:747122/143‑1 (MQ=255)

                                                                                 aCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGGAGATGTCGTAATCCTGTGCCGCtcta                                                   >  1:685471/1‑140 (MQ=255)

                                                                                  cccGGAGAAGTTTTTCGGTGACGCGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACGTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                  <  2:673361/143‑1 (MQ=255)

                                                                                    cGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAAGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACCCCCCCACCACCCGCAGGAACATAGCGTAATACAATGCCGCTCGCAGt                                                >  2:403449/1‑143 (MQ=255)

                                                                                     ggAGAATTTTTTCGGTGACGAGGTGATCTGCATCTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                               <  1:156268/143‑1 (MQ=255)

                                                                                        gaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCgg                                            <  1:562672/143‑1 (MQ=255)

                                                                                          aTTTTTTCGGTCACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTa                                          <  1:811798/143‑1 (MQ=255)

                                                                                                 cgttGACGGGGTGGTCTGCCTTTCTGCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGTAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaaca                                   <  2:675916/140‑1 (MQ=255)

                                                                                                     gtcGAGGTGCTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCGACTTCCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                               <  2:546621/141‑1 (MQ=255)

                                                                                                           gtgtcttGCATTTCGCCGTGATTGTTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCTGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt                         <  2:608988/137‑1 (MQ=255)

                                                                                                              aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCGTTGaa                      >  1:812472/1‑143 (MQ=255)

                                                                                                                ctctaTTTCACCGTTGTTGTTATGCAGTCTCTGCGTCGCCTCTTGTACTTTCCACGTTTGTCCGTACTCCACCACCATTCGCAGGAATATTTCGTAATCCTGTGCCGCTTTCATTTCGGTATCGAACAAACACTCTTTGAAAc                    <  2:307217/139‑1 (MQ=255)

                                                                                                                                ttgatATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCCCTGTTCGCCGCAGTCCACCACCATCCGCAGGAAGATGTCGTACTCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa    <  2:115179/143‑1 (MQ=255)

                                                                                                                                 tgctatGCAGGCTCTGCGTCGCCTCTGCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAg   <  2:748748/140‑1 (MQ=255)

                                                                                                                                  gatACGCAGGCTCTGCGTCCCCTCTTCTCCTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGa  <  1:167877/143‑1 (MQ=255)

GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGA  >  NC_000913/2132647‑2132919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A