Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A24 F184 I2 R1	15	56.1	2030262	91.3%	1853629	141.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	150.4 / NA	49	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (31/18); total (31/18)

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG  >  NC_000913/2132650‑2132924

tCTGGTAGAGGGTAAACAGCTGATATTTCTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                      <  1:710197/143‑1 (MQ=255)

  tcgTAGAGGGTAAACAGCTGATATTTCTTGCTGGCGCGGTCGCCTTTGTCTTCGTGCTTGCGGTAAGNGCGGAAGTACCCGGACAATTTCCTCGGCGACCAGGTGATCTGCATTCCACCGTCACTGACCTGCACGCTCCGCGt                                                                                                                                    >  2:575599/3‑143 (MQ=255)

   ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                   <  1:676824/143‑1 (MQ=255)

          ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt                                                                                                                            <  2:672792/143‑1 (MQ=255)

               aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGTTCTGCATTTCACCGTGCTTGATATGCAGGCTCTACGTCGActcgcctagt                                                                                                                       >  1:84744/1‑136 (MQ=255)

               aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATCTGCCGGCTCTGCGTCGCCTCGTCTGCt                                                                                                                       >  1:746880/1‑143 (MQ=255)

               aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACt                                                                                                                       >  1:708153/1‑143 (MQ=255)

                catgtgctatTTTTTGCTGGCGCGGGCGAATTTGGCTGTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt                                                                                                                      <  2:288458/136‑1 (MQ=255)

                    tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                  >  1:396632/1‑143 (MQ=255)

                         ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                             >  1:343253/1‑143 (MQ=255)

                         ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                             <  2:889712/143‑1 (MQ=255)

                          tttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTc                                                                                                            >  1:491030/1‑143 (MQ=255)

                                       ggTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacc                                                                                               >  1:778488/1‑143 (MQ=255)

                                         tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGCATTTTTTCGGTGCCGAGGTGAGCTGCATTTCACCGTGATTGATATGCAGGCGCTGAGTCGCCTCTTCTACTGTCCACGGTTCGCCCTACTctacgac                                                                                             >  2:736078/1‑143 (MQ=255)

                                                     ttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGATGTTTGTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                                 <  2:604035/143‑1 (MQ=255)

                                                       gtgCTCGCGGTAAAAGGGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGa                                                                               <  1:895816/143‑1 (MQ=255)

                                                             gCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGAAGGCTCTGCGTCGCGTCTTATACGTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGt                                                                         >  2:212770/1‑143 (MQ=255)

                                                             gCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCATGATTGATATGCAGGCTCTGCGTCGCCTCTTCGACTTTCCACGGTTCGCCGGACTCCCCCCACATCCGCAGGAAGCTGTCGt                                                                         >  2:326805/1‑143 (MQ=255)

                                                               ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCATCTTCTACTTTCCACGGTTCGCAGTACTCCACAAACATCCGCAAGAAGATGtagaaa                                                                       >  2:287426/1‑138 (MQ=255)

                                                                gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCGTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGAGGTCGTAAt                                                                      >  2:641619/1‑143 (MQ=255)

                                                                 tAAAAGTGGAAGTACCCGGAGACTTTTTACGGTGACGAGGTGATCTGAATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTACTACTATCGACGGATCGCGGTACTGCACCACACCCCGCACGACGATGGCGCAATc                                                                     >  2:403005/1‑143 (MQ=255)

                                                                 tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTACTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGGTGTCGTAATc                                                                     >  2:717246/1‑143 (MQ=255)

                                                                     aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                                 <  2:708153/143‑1 (MQ=255)

                                                                      gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCCCTGCGTCGCCTCTTCTACTCCCCCCGGTCCGCCGTACTCCACCACCACCCGCCGGACGCCGCCGCAATCCtgtg                                                                >  1:941208/1‑143 (MQ=255)

                                                                           aGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCt                                                           <  2:343253/143‑1 (MQ=255)

                                                                               cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                       <  1:477912/143‑1 (MQ=255)

                                                                                ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGCTGTCGTCATCCTGTGCCGCTTTCAg                                                      >  1:636068/1‑143 (MQ=255)

                                                                                   gttcaTTTTTTCGTTGACGAGGGGATCTGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc                                                   <  2:579520/139‑1 (MQ=255)

                                                                                   gagaATTTTTTCGGTGACGAGGTGATCTGCATTGCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc                                                   <  2:809637/143‑1 (MQ=255)

                                                                                         tttttCGGTGACGAGGTGATCTTCATTTCACCGTGATTGATATGCAGGCTCTGCGTTGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGGAGGAGGATGTCGTAATCCTGTACCGCTTTCCATTCGATATc                                             >  2:768188/1‑143 (MQ=255)

                                                                                                       ggTGATCTGCATTGGAGCGGGCTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACTCCACCAGCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt                               <  1:837860/143‑1 (MQ=255)

                                                                                                        gTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTTTCGAACAAACAGTCttt                              >  2:1014193/1‑143 (MQ=255)

                                                                                                         tGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTg                             >  1:249511/1‑143 (MQ=255)

                                                                                                            tgtGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                          <  2:743243/141‑1 (MQ=255)

                                                                                                            tCTGCATTTCACCGTGATTGATCTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCTTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                          <  2:819461/143‑1 (MQ=255)

                                                                                                             cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccac                                                                                                >  2:393062/1‑72 (MQ=255)

                                                                                                             cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccac                                                                                                <  1:393062/72‑1 (MQ=255)

                                                                                                             cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAGGCAGATGTCGTACTCCTGTGCCGCCCTCAGCTCGGTCCCGACCAACCCCCCtttcagac                         >  1:447702/1‑138 (MQ=255)

                                                                                                               gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCCCCCTACTATATCTGCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGc                       >  1:637899/1‑143 (MQ=255)

                                                                                                                 aTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                         <  1:811112/107‑1 (MQ=255)

                                                                                                                 aTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                         >  2:811112/1‑107 (MQ=255)

                                                                                                                      aCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGACACGCCATGccc                >  1:677370/1‑143 (MQ=255)

                                                                                                                            attgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACACACACTCTTTGAAACGACATTCCCAGGTaa          >  2:922060/1‑143 (MQ=255)

                                                                                                                            attgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGCAACGCCATGCCCCGGTaa          >  2:154610/1‑143 (MQ=255)

                                                                                                                              tgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGACCAAACACTCTTTGAAACGCCATGCCCAGGTAAAg        >  1:650061/1‑143 (MQ=255)

                                                                                                                               gatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGa       >  2:184838/1‑143 (MQ=255)

                                                                                                                                   tGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTAATCCTCTGCCGCTTTCAGTTCGGTATCGAACAAACACCCTTTGAAACGCCCAGCCCAGGCaacgccccg   >  2:183638/1‑136 (MQ=255)

                                                                                                                                   tGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTg   >  1:561433/1‑143 (MQ=255)

                                                                                                                                    gCAGGCGCTGCGTCGCCTCTGCCACTTTCCTCGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGGAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTgg  <  2:396632/143‑1 (MQ=255)

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG  >  NC_000913/2132650‑2132924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A