Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A23 F184 I2 R1
|
13 |
42.0 |
1528396 |
89.7% |
1370971 |
142.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,560,455 |
+G |
intergenic (‑2/+1) |
glpR ← / ← glpR |
pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,560,455 | 1 | . | G | 97.1%
| 94.4
/ ‑3.7
| 35 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (21/13); total (22/13) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGA > NC_000913/3560316‑3560588
|
gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGcgca > 1:262216/1‑140 (MQ=255)
aGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAg > 2:47656/1‑143 (MQ=255)
gacgcATTGGGGTGTTGCTCCGCCACTTGGCGGGCGAGGCGCTCTGTTTCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGCAGACCTGTCACCTCCGCCATGAT‑TGCGCAGGATCAGGTTTTg < 2:56598/139‑1 (MQ=255)
cATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGAGCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGGTTTGCTcc > 1:65774/1‑143 (MQ=255)
ggggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGAT‑GGCGCAGGATCAGGTTCGGCTCCGCCa > 2:559916/1‑143 (MQ=255)
tGCGCCGCCACTTTGCGGGCTATGCGCTCTTTTTCTTCGCTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt < 2:726591/143‑1 (MQ=255)
gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTACCCGAACTGGAAGGCAGCGCCGCACCGCAAAGAG‑GGCGCAGGATCAGGTTCTGCTCCGCCAGCTCATTg > 2:45704/1‑143 (MQ=255)
gtctgCCACTTTGCGGGTGATGCGCTCTTTGTCTTCGGGCTGGGGGGCCCTGCGGTCGTGCCGCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGa < 2:158030/139‑1 (MQ=255)
tCCGCCACTTTGCGCGCTATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGCCGTGTTCACCGAACTGGAAGGCAACGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGGCAGCTCCTTGAg > 2:96995/1‑143 (MQ=255)
cGCCACTTTGCGGGCGATGCGCTCTTTTTCTACGCTCTCGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGCACTGGAAGGCAGCGCCGCACCGCCATGCTCGCC‑CAGGATCAGGTTGCGCTCCGCCAACCCAtccagct > 2:579159/1‑137 (MQ=255)
ccACTTTGCGGGCGATGCGCTCCTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg < 1:467067/143‑1 (MQ=255)
cctcacGATGCGCTCTCTTCCTTCGGTCGGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa < 1:50049/138‑1 (MQ=255)
gcgctgcgctCTTTTTCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGt < 2:26965/143‑1 (MQ=255)
tGCGGTCTTTTTGTTCGGTCTGTGTGGCCTTGCGAGCGCGCCACGGCGTGTTAACCGAACTGGAAGGCCGCGCCTCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc < 2:82463/143‑1 (MQ=255)
tctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCACTGCGGTCGCGGCGAGTAGCCCGCggaca > 2:433269/1‑140 (MQ=255)
tcGGTCTGGGTGGCCTTGCGATCGTGCAACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTATGCGGGCTGACGGCGaa > 2:585063/1‑143 (MQ=255)
ggTGGCCTTGCGATAGTGCCACGGCGTGTTAGACGAACGGCAAGGCAGCGCCGCACCGCCATGAG‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGGCGCGTCGAATAGTCAGCGGGCTGACGGAGAAAGGCTCTa > 2:14668/1‑143 (MQ=255)
ccTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGc < 1:47656/143‑1 (MQ=255)
cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGACATGCTCTACCAGct > 2:202158/1‑143 (MQ=255)
gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg > 1:717123/1‑143 (MQ=255)
gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAACGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg > 1:492555/1‑143 (MQ=255)
aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGGAGCGCCGCACCGCCATGAT‑GGCGCCGGATCAGGTTTTGCTCCGCCAGCCCATTGACGTCGCGGCGAAGAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCGTCgg > 2:355954/1‑143 (MQ=255)
ccACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTGGCTCCGCCCGCCCCTTGAGGTCGCGGCGAACAGTCTGCGGGCCGACGGAGACACGCGCCACCAGCTCCTCGGCAcggg > 2:226478/1‑140 (MQ=255)
aCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACa > 1:538944/1‑143 (MQ=255)
tgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAccc > 1:392288/1‑143 (MQ=255)
gtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCAGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGGCATGCTCTACCAGCTCTGCGCTACTGACATAACCct > 1:286300/1‑143 (MQ=255)
aCCGAAGTGGAAGGCAGGGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgctg < 2:749790/143‑1 (MQ=255)
gAACTGGAAGGCGGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCACTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt < 1:54023/143‑1 (MQ=255)
aattGGGAGGCAGCGCCGCCCCGCCATGAT‑GGCGCAGGGTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTa < 2:93850/140‑1 (MQ=255)
gAAGGCAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAACTACGTCGAGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCGCTTCGGTGCTGACATGACCGTTCTGTTTacgcag > 2:160873/1‑138 (MQ=255)
cAGCGCCGCACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGCCTAGCCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACCTAACCCTGCTGTTTAACCAGTTCgc > 1:189560/1‑142 (MQ=255)
aGCGCCGCACCGCCATGAT‑CGCGCCGGACTAGGCGGGGTCCCGCCAGCGCCTTGAGGCCGCGGCGAATAGGCTGCGGGCTGACGGAGAAATGCTCTACCCGCTCTTCGCTACTGACATAACCCTGCTGTTTAACCAGCTCgct > 2:544406/1‑141 (MQ=255)
ccgcACCGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTATACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAGCCAGTTCGatcata > 1:117211/1‑143 (MQ=255)
cGCCATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtg > 1:429764/1‑143 (MQ=255)
gcATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTGGGTGCTGACACAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgtg < 1:322048/142‑1 (MQ=255)
cATGAT‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGa < 1:618412/143‑1 (MQ=255)
|
GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAT‑GGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGA > NC_000913/3560316‑3560588
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
