Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F109 I2 R1	18	64.5	2321652	91.0%	2112703	142.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,020,956	A→G	intergenic (‑37/‑182)	sulA ← / → sxy	SOS cell division inhibitor/CRP‑S‑dependent promoter expression factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,020,956	0	A	G	100.0%	145.2 / NA	45	intergenic (‑37/‑182)	sulA/sxy	SOS cell division inhibitor/CRP‑S‑dependent promoter expression factor
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/20); total (25/20)

TGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTA  >  NC_000913/1020820‑1021095

tGATAAGCCCGGCTGTAGTGTTTTCCGTAGAAACACGCGCAATTTTACTTGCTGCGGATGAGAAAGACGAAGAACGATGTGCATAGCCTGAAGGGTACATAATCAATCAAGCCCCTGAGAGTTACTGTATGGATgtgcagtac                                                                                                                                       >  2:600259/1‑143 (MQ=255)

   tAAGCCTGTTTGTCGTGTCTTCCGCAAGTACACGCGCAATTTTCCTTGCTACGGATGACAACGACAAAGATCGATGTGCATAGCCTGAAGTGTACCTATTCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATc                                                                                                                                    <  2:67942/143‑1 (MQ=255)

    atgCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATcc                                                                                                                                   <  2:152036/141‑1 (MQ=255)

      gCCCGGCTGTAGTGTTTTCCGGAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAg                                                                                                                                 <  2:22890/143‑1 (MQ=255)

       cccGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCact                                                                                                                                >  2:1144145/1‑141 (MQ=255)

       cccGGCTGTAGTGTTTGCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGt                                                                                                                                <  1:1059134/143‑1 (MQ=255)

             tGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGacaac                                                                                                                          >  1:1015488/1‑143 (MQ=255)

              gTAGTGTGTTCCGGAGAGACACGCGCAATTGTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGacaaca                                                                                                                         <  2:212329/143‑1 (MQ=255)

               tAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGAcaacaa                                                                                                                        <  1:898194/143‑1 (MQ=255)

                      ttCCGTAGAGACACGCGCCATTTTACTTTCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAATTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCaa                                                                                                                 <  2:696173/143‑1 (MQ=255)

                           tAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTa                                                                                                            >  1:953500/1‑143 (MQ=255)

                                    gcgcAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGaa                                                                                                   <  1:795042/143‑1 (MQ=255)

                                    gcgcAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGAGAGTTACTGTATCGCTGTGCAGTACATCCAGTGACAACAAAGAACAACCCTAttatagaaa                                                                                                   >  2:1054146/1‑136 (MQ=255)

                                        aaTTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAagag                                                                                               <  1:887746/143‑1 (MQ=255)

                                        aaTTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAagag                                                                                               >  1:1108104/1‑143 (MQ=255)

                                                ttGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCAGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCaa                                                                                       >  2:473430/1‑143 (MQ=255)

                                                    tgcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAAttt                                                                                   >  1:883158/1‑143 (MQ=255)

                                                           gagaACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGtt                                                                            <  1:771352/143‑1 (MQ=255)

                                                              aaCGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCACCCCTATTTTCGGAAAGCGACTCGCACCTTTCGTCGTTggc                                                                         >  2:1026298/1‑142 (MQ=255)

                                                                cgacgaAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCGCGCAAATTTTGTCGTTGGTGa                                                                       >  2:635960/1‑143 (MQ=255)

                                                                     aagaaCGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAACGATCCACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGa                                                                  >  1:902679/1‑143 (MQ=255)

                                                                      agaaCGATGTGCATAGCCTGAAGTGTACATAATCAAGCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGaa                                                                 <  2:635287/143‑1 (MQ=255)

                                                                       gtaCGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGaaa                                                                <  1:890154/141‑1 (MQ=255)

                                                                       gaaCGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAGCCACGGTTAACCCTAGCTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGaaa                                                                >  2:820202/1‑143 (MQ=255)

                                                                          cGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACa                                                             >  2:670156/1‑143 (MQ=255)

                                                                             tgtgCATAGCCTGAAGTGTACATAATCAATCCAGAGCATGCGGGCGACTGTATCGATGTGCAGTACATCCAGTGACAACAAAGATCAACCATATATTCGGAAAGAGCCTCGCAAATTTTGTCGTTCATGACGGGCAAACAtca                                                          >  2:248511/1‑141 (MQ=255)

                                                                                  aTAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGCACGTGCCTCGCGTATTTTGTGGTTGGTGACCGGAAAACATAAATTaa                                                     >  1:96283/1‑143 (MQ=255)

                                                                                   tAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAAt                                                    <  2:372399/143‑1 (MQ=255)

                                                                                   tAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAAt                                                    <  1:701809/143‑1 (MQ=255)

                                                                                      ccTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCACACTCTGTCGTTGGTGACGGGAAAACATAAATTAATCtt                                                 <  1:599991/143‑1 (MQ=255)

                                                                                      ccTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGGAAACATAAATTAATCtt                                                 >  1:1072278/1‑143 (MQ=255)

                                                                                          aaGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGccc                                             >  2:1016416/1‑143 (MQ=255)

                                                                                              gtACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTa                                         >  2:32362/1‑143 (MQ=255)

                                                                                               tACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTaa                                        <  1:672227/143‑1 (MQ=255)

                                                                                                  aTAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTACGaa                                     >  1:379216/1‑143 (MQ=255)

                                                                                                   tAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAAt                                    <  1:736332/143‑1 (MQ=255)

                                                                                                     atcaatcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATaa                                  >  2:75066/1‑143 (MQ=255)

                                                                                                     atcaatcCAGCCCCTGTGAGTTACTGTATGGAAGTGCAGTACATCCAGTGAAAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAGATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGTATaa                                  >  2:603510/1‑143 (MQ=255)

                                                                                                      tcaatcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAg                                 <  2:479495/143‑1 (MQ=255)

                                                                                                         atcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTg                              >  2:816918/1‑143 (MQ=255)

                                                                                                          tcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGATAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGc                             <  1:748188/143‑1 (MQ=255)

                                                                                                            cAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCt                           >  1:404121/1‑143 (MQ=255)

                                                                                                                         ttACTGTATGGACGTGCCGTACATCAAGTGACAACGAAGATCAACCCTGTTTTCGGAAAGAGCCTCGCATTTTTTCTCGTTGATGACGGGATAACATAGATTGATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTa              <  2:764158/143‑1 (MQ=255)

                                                                                                                               tATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGAt        >  2:492625/1‑143 (MQ=255)

                                                                                                                               tATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTCCCTATGTTCGTAGTTAAccaag        >  2:82990/1‑139 (MQ=255)

                                                                                                                                aTGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATc       >  1:620470/1‑143 (MQ=255)

                                                                                                                                     tgtgCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATATATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTa  <  2:724514/143‑1 (MQ=255)

TGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTA  >  NC_000913/1020820‑1021095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A