Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F113 I1 R1	17	62.1	2245352	90.5%	2032043	142.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,020,961	A→G	intergenic (‑42/‑177)	sulA ← / → sxy	SOS cell division inhibitor/CRP‑S‑dependent promoter expression factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,020,961	0	A	G	98.3%	185.5 / NA	59	intergenic (‑42/‑177)	sulA/sxy	SOS cell division inhibitor/CRP‑S‑dependent promoter expression factor
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (33/25); total (34/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGAT  >  NC_000913/1020825‑1021089

aGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCa                                                                                                                            >  2:487003/1‑143 (MQ=255)

  cccGGCTGTAGTGTTTTCCGTAGAGACACGCGCCATTTTACTTGCTGCGGATGAGAACGACGCAGAACGAGGTGCATCGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGGGTTACCATCTGAATGTACGGTGCACCCAgc                                                                                                                          >  2:325682/1‑142 (MQ=255)

   ccGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCGGTg                                                                                                                         >  2:936566/1‑143 (MQ=255)

   ccGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTg                                                                                                                         >  1:183463/1‑143 (MQ=255)

    cGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGa                                                                                                                        <  2:758600/143‑1 (MQ=255)

    cGGCTGTAGGGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGTAAGGAGTTCAAACCCTGATGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGa                                                                                                                        <  1:258242/143‑1 (MQ=255)

       cTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGacaa                                                                                                                     >  2:1112360/1‑143 (MQ=255)

           ttggtTTTCCTTAGAGACACGCGCAATTTTACTTGCGGCGTATGATAACGACGAAGAACGATGTGCAGAGCCTGAAGTATACATAAGCAATCCAGCCCCTGTGAGTTATTGTATGGATGTACAGTGCATCCAGAGACAACaaa                                                                                                                 <  2:461383/140‑1 (MQ=255)

              gtTTGCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGAt                                                                                                              <  1:279803/143‑1 (MQ=255)

               ttgtCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGCACGACGAAGAACGATGTGCATAGCCTGACGTGTACATAATCAATCCAGCCCCTGTGACGTACTGTGTGGCTGTACAGTGCACCCCGTTGCAACAAAGATc                                                                                                             >  2:750597/4‑143 (MQ=255)

                 ttCCGTAGAGACACGCGCAATCTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCaa                                                                                                           <  1:692909/143‑1 (MQ=255)

                  tCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAAc                                                                                                          <  1:373854/143‑1 (MQ=255)

                  tCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGCTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAAc                                                                                                          >  2:890849/1‑143 (MQ=255)

                   ccGTAGAGACACGCGCAACTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATCGATGTACAGTGCATCCAGTGACAACAAAGATCAAcc                                                                                                         <  1:721246/143‑1 (MQ=255)

                     gTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGCGTTACTGTATGGATGTACAGTGCAACCAGTGACAACAAAGATCAACCCt                                                                                                       >  2:53563/1‑143 (MQ=255)

                     gTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACACCAAAGATCAACCCt                                                                                                       >  1:440058/1‑143 (MQ=255)

                     gTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGACTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGCTGTACAGTGCATCACGTGACAACACAGATCAACCCt                                                                                                       >  2:941737/1‑143 (MQ=255)

                           acacGCGCAAGTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTc                                                                                                 <  1:519488/143‑1 (MQ=255)

                            cacGCGCCATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCg                                                                                                <  2:619884/143‑1 (MQ=255)

                                  tagttttACTTGCTGCGGATGGGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCGTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAaga                                                                                          <  2:469951/140‑1 (MQ=255)

                                  gaaTTTTACTTGTGGCGGCTGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCACTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAaga                                                                                          <  2:260083/142‑1 (MQ=255)

                                   aaTTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCGTGCAGGGTACATAGTCAATACAGACCCTGTGAGTTACTGTATGGATGTACAATACATCCAGTGAACACAACTATCAACCCTCTTCTCGCAAcgag                                                                                         >  2:1050961/1‑143 (MQ=255)

                                      tttACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCt                                                                                      <  2:628578/143‑1 (MQ=255)

                                           ttGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACGACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCaa                                                                                 >  2:768944/1‑143 (MQ=255)

                                           ttGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGCGCATCCAGTGCCAACAAAGATCAACCCTATTTTCGGACAGAGCCTCGCaa                                                                                 >  2:773252/1‑143 (MQ=255)

                                           gtgatgcGGATGAGAACGACGAAAAACAATGGGCATAGCCTGAAGTGTACATAATCAATACAGCTCCTGTGAGTTACTGTATGGATGAACAGAGCATCCAGAGAGAAAAAAGATCAACACTATTTTCGGAAAGAGCCTCGCaa                                                                                 >  2:614068/5‑143 (MQ=255)

                                               tgcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAAttt                                                                             >  1:1083065/1‑143 (MQ=255)

                                                gcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAAtttt                                                                            >  1:706321/1‑143 (MQ=255)

                                                   gATGAGAACGACGAAGGACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTc                                                                         <  2:43301/143‑1 (MQ=255)

                                                       agaACGACGAAGAACGATGTGCATAGCCTGAAGGGTACATAATCAAGCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTg                                                                     <  1:11718/143‑1 (MQ=255)

                                                           cgacgaAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTCAGGAAAGAGCCTCGCCACTACTGGCCTTGGTGa                                                                 >  2:782651/1‑143 (MQ=255)

                                                             acgaAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACg                                                               >  1:985557/1‑143 (MQ=255)

                                                             acgaAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACg                                                               >  1:771159/1‑143 (MQ=255)

                                                                  gaaCGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGaaa                                                          >  2:26129/1‑143 (MQ=255)

                                                                  gaaCGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAAATTTGTCATTGGGGACGGGaaa                                                          >  2:874032/1‑143 (MQ=255)

                                                                    ccGATGTGCATAGCCTGAAGTGTACATAAACAATCCCGCCCGTTTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAAc                                                        <  1:1056232/142‑1 (MQ=255)

                                                                      gATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACAt                                                      <  1:1016211/143‑1 (MQ=255)

                                                                      gATGCGCATAGCCTGAAGTGTACAGAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACGACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACAt                                                      <  1:382736/143‑1 (MQ=255)

                                                                         gtgCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTCTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATaaa                                                   <  1:601644/143‑1 (MQ=255)

                                                                           gCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAAtt                                                 >  2:1874/1‑143 (MQ=255)

                                                                           gCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGAAGGGGACGGGGAAACGTAACtt                                                 >  1:148317/1‑143 (MQ=255)

                                                                            cATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTa                                                >  1:161828/1‑143 (MQ=255)

                                                                               aGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGGTACTGTATGGATGGACAGTGCATCCAGTGACTACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATc                                             <  1:317450/143‑1 (MQ=255)

                                                                               aGCCTGAACTGTATATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATc                                             <  1:970353/143‑1 (MQ=255)

                                                                                 ccTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATACATTAATCtt                                           >  2:517991/1‑143 (MQ=255)

                                                                                            cATAATCAATCCAGCCCCTGTGAGTTACTGTCTGGATGTACAGTGCACCCCCTCACAACAAAGATCAAACCTATCTTCTGAAAGAGCCTCGCAAATTTTGTCGTTGCTGACGCGAAAACATAAATTAATCTTGCCCCTTAAGa                                >  2:909559/1‑143 (MQ=255)

                                                                                              tAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTCAGact                              >  2:856728/1‑141 (MQ=255)

                                                                                                atcactcCAGTCCCTGCGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATaa                            <  1:41122/143‑1 (MQ=255)

                                                                                                atcaatcCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAAAAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCCAATTTTGTCGTTGTTGACGGGCACACATAAATTCATCTTGCCCCTTCAGAATag                            >  1:911745/1‑142 (MQ=255)

                                                                                                   aatcCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGtt                         <  1:174745/143‑1 (MQ=255)

                                                                                                     tcCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGCATACATAATTTAATCTTGCCCCTTAAGAATAAGTTGc                       >  1:203964/1‑143 (MQ=255)

                                                                                                       cAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCt                     >  1:321029/1‑143 (MQ=255)

                                                                                                          ccccTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATCAATTAATCTTGCCCCTTAAGAATAACTTGACTAtt                  >  2:40558/1‑143 (MQ=255)

                                                                                                              ggcgAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCg              <  1:1026237/140‑1 (MQ=255)

                                                                                                                      aCTGTATGGATGTACAGTGCCCCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAAc      <  1:865556/143‑1 (MQ=255)

                                                                                                                      aCTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAAc      >  1:150742/1‑143 (MQ=255)

                                                                                                                       cTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACg     >  2:705041/1‑143 (MQ=255)

                                                                                                                        tGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTGACgg    >  1:972525/1‑143 (MQ=255)

                                                                                                                          tagggATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTGGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGAt  <  2:604076/140‑1 (MQ=255)

AGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGAT  >  NC_000913/1020825‑1021089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A