Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F18 I0 R1 150 105.7 2655328 79.3% 2105675 226.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,480,296 T→C 21.1% G104G (GGA→GGG)  kefB ← potassium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,480,2960TC21.1% 169.1 / 6.0 76G104G (GGA→GGG) kefBpotassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (48/8);  new base C (2/16);  total (50/26)
Fisher's exact test for biased strand distribution p-value = 1.26e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCAAATATCCCAACACAGCGC  >  NC_000913/3480060‑3480519
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                 
aCCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCCGTTGAAGACATCGCAAGGCCAATGCCACCAACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAAAAAT‑c                                                                                                                                                                                                                                 >  1:984138/1‑238 (MQ=255)
aCCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCACCATCAATAAT‑c                                                                                                                                                                                                                                 >  1:947338/1‑238 (MQ=255)
aCCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCAAGCGGAAATCCGTCAGCATCAATAAT‑c                                                                                                                                                                                                                                 >  1:44162/1‑238 (MQ=255)
      aTCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCa                                                                                                                                                                                                                           >  1:587663/1‑238 (MQ=255)
            tCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGCCCACCGCCGCCTGCCAGGCGAAACCCGCCAGCATCAATAAT‑CCCGCCAGCAAcg                                                                                                                                                                                                                     >  2:813364/1‑238 (MQ=255)
            tCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAAcg                                                                                                                                                                                                                     >  1:1046092/1‑238 (MQ=255)
                          tGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCCACGCCATTGCAGTTGAAGCCATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAAACCGTCATCATCAATAAT‑CCCGCCAGCAACCCCGCGCTTAAAAga                                                                                                                                                                                                       >  1:485755/1‑237 (MQ=255)
                                   cGCCAACAACGGCACTAACGCCAGTGCTGGGCTTACTGCCAGATCCTGAAACAGCAGCACCGACAACCCGAGCTGGCCTGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATGGCAATGCCAATGCCACCGCCCACCGCCGCCTGCCAGGCCAAATCCGCCCGCATCCATACT‑CCCGCCTGCCACCACGCGCTT‑ACAGCACCCTGTGcc                                                                                                                                                                                             >  2:802850/1‑238 (MQ=255)
                                   cGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGCCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGcc                                                                                                                                                                                             >  1:1073417/1‑238 (MQ=255)
                                               cACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGccca                                                                                                                                                                                >  1:689662/1‑237 (MQ=255)
                                               cACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACCCCaa                                                                                                                                                                                >  1:850619/1‑239 (MQ=255)
                                               cACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAC‑CCCGCCAGCAACCCCGCGCTTAACAGCA‑CCCTTACCGCGCCCACCCCaa                                                                                                                                                                                >  2:750085/1‑239 (MQ=255)
                                                         aGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCCCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCCGCAACCCCCCGCTTAACACCC‑CCTGTCCCCCCCCTCCCCCaaaaacggaag                                                                                                                                                                       >  2:871708/1‑232 (MQ=255)
                                                         aGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCCCCTGCCAGGCGAAAACCGTCCGCCTCCATAAT‑CCCCCCAGCAACCCCCCCCTATAAACAA‑CCCGTCCCCCGCCCACCACAAAAACCGaaa                                                                                                                                                                       >  2:871707/1‑237 (MQ=255)
                                                               gggATTACTGCCAGATCCGAAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGGGATTCATCCCTTTCCCCCCCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCa                                                                                                                                                                 <  1:1106405/238‑1 (MQ=255)
                                                                     aCTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGCAATCCGTCAGCATCAATAAC‑CCCGCCCGCAACGCCCCGCTTAACACCA‑CCCGTGCCCCCCCTCCCCCAAAAAAACAACGCCGCaatggcaa                                                                                                                                                          >  2:1277304/1‑232 (MQ=255)
                                                                     aCTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTaa                                                                                                                                                                                                           >  1:1139896/1‑191 (MQ=255)
                                                                     aCTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCa                                                                                                                                                          >  1:913652/1‑239 (MQ=255)
                                                                     aCTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCACTTGCCa                                                                                                                                                          >  1:1005936/1‑239 (MQ=255)
                                                                     aCTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACACCGCAGTTGccc                                                                                                                                                          >  1:1098079/1‑238 (MQ=255)
                                                                            gATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTAAAGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCCCAAGGCCAATGCCCCCGACCACCGCACCCTGCGAGGCGAAATCCGTCAGCATCAAAAAT‑CCCGCACCCAACGCCGCGCTTCAACGCA‑CCTGTGCCGCCCCAACCCCAGAAAACGAACCCCTCACttcgaaaaatgtg                                                                                                                                                   >  2:1074367/1‑228 (MQ=255)
                                                                                   aaaCAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGAt                                                                                                                                            >  1:855497/1‑239 (MQ=255)
                                                                                    aaCAGCAAACCCGAAAGCCCGAGCTGGCCGGATTCGCTGCGATTCACCCCTTTCCCCCGCATCA‑ATTGCACCGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGAtt                                                                                                                                           <  1:413310/239‑1 (MQ=255)
                                                                                         cagAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAAc                                                                                                                                       >  1:1254538/1‑238 (MQ=255)
                                                                                         cagAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACCCCGCAGTTGCCAAAGTTTGGAGGGATTCAAc                                                                                                                                       >  1:201924/1‑238 (MQ=255)
                                                                                          caaccacaaaaCCCGGGGTGGCCGGTTTCTGTCGTATTCATCCCCTTCCCACCCACCA‑ATTGCAACCCCATTAGAGTGGAAACCACCGCAAGCCAAATGCCACCGCCCCCCGCCGCCTGCAGGGCGAAATCCGCCAGAATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTc                                                                                                                                     <  2:587663/232‑1 (MQ=255)
                                                                                           gAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGACGTAGCCAAAGATTGGAGGGATTCAACTCa                                                                                                                                    >  1:876977/1‑239 (MQ=255)
                                                                                               ccaaacCCCTAGCTGGCGGGATTCGCGTCGTTCCACCCCTTTCTCCCGC‑TCACATTGCAACCCCGTTGACGTGGAAGACCTCGCAAGGCCCATCCCCCCGACCACCGCCGCCCGCCAGGCAAACTCCGTCAGCATCAATAAC‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGc                                                                                                                                <  2:947338/237‑1 (MQ=255)
                                                                                                              gCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCCCAGTTGCCAAAGTTTGGCGGGGTTCAACTCAGGGCCGTTGATAAACATCa                                                                                                                 >  1:1080270/1‑239 (MQ=255)
                                                                                                                     tCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATa                                                                                                           >  1:701213/1‑238 (MQ=255)
                                                                                                                             aTTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCCCCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCcgcg                                                                                                                                                                                                                <  1:950134/130‑1 (MQ=255)
                                                                                                                             aTTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCcgcg                                                                                                                                                                                                                >  2:950134/1‑130 (MQ=255)
                                                                                                                                      tttcccccccATCA‑ATTCCAACCCACTTGCAGTTGAAGACACCCCAAGGCCAATGCCCCCCACCGCCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAC‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGcaa                                                                                         <  1:715009/230‑1 (MQ=255)
                                                                                                                                        tctcACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGACCGCCGCAGTTGCCAAAGTTTGGAGGGCTTCAACTCAGGGCCGTTGCTAAACATCAGGAATACCACGCCGAGTTCCGaaaaa                                                                                       >  1:939300/1‑238 (MQ=255)
                                                                                                                                        tctcACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCACCTCAAGGCCGAGGATAAACATCAGGAATACCACCCCGAGTTCCGaaact                                                                                       >  1:680983/1‑237 (MQ=255)
                                                                                                                                        tctcACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAAc                                                                                                                                       >  1:586798/1‑191 (MQ=255)
                                                                                                                                                ataa‑aTTGCAAGCCCATTGCAATTGAAGAAAACGAAGGCCCACGGCCCCCGCCCACCGCCGCCGGCCAGGCGAAATCCGGCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGAt                                                                               <  1:556841/236‑1 (MQ=255)
                                                                                                                                                     aTTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCCAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTggaggactcca                                                                           >  1:1034435/1‑234 (MQ=255)
                                                                                                                                                           aCGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGCCg                                                                     >  1:840980/1‑239 (MQ=255)
                                                                                                                                                           aCGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGGGGAGGATCTCATCGAc                                                                      >  1:601369/1‑238 (MQ=255)
                                                                                                                                                            ccccATTGGAGTTAAAGACCTCCGAAGGCCAACGCCCCCGCCCCCCGCCGCCTGCCGGGCAAAATCCGCAAACAACAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGt                                                                    <  1:871708/237‑1 (MQ=255)
                                                                                                                                                            cGCCATTGCAGTTGAAAAAAACACAAGGACAATGCCCCCGCCCCCCGCACCCGGCCAGGCAAAACCCGCCAGACTCAATATA‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGGGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGt                                                                    <  1:871707/239‑1 (MQ=255)
                                                                                                                                                              ccATTGCAGTTGAAGACATCGAAAGGCCAATGCCACCGACCCCCGCCGCCTGCCGGGCAAAACCCGTCAGCATAAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCg                                                                  <  1:813364/239‑1 (MQ=255)
                                                                                                                                                                 ttGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGCGGATCTCATCGACGCCCCta                                                               >  1:885625/1‑239 (MQ=255)
                                                                                                                                                                  tGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGCGTTCCGAAAAGTGGGGGATATCATCGACGTCGCtaa                                                              >  1:1071989/1‑239 (MQ=255)
                                                                                                                                                                         gAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGGTCCCATCGACGTCGCTAATAAAcc                                                        >  1:1208165/1‑238 (MQ=255)
                                                                                                                                                                         gAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGAACTCATCGACGTCCCTAATAAAccc                                                       >  1:80233/1‑239 (MQ=255)
                                                                                                                                                                         gAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAAAATCAGGAATACCACGCCGAGTTCCGAAAATGGGAGGATCTCATCGACGTCGCTAATAAAcc                                                        >  1:1021092/1‑238 (MQ=255)
                                                                                                                                                                              cATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAAAAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCCGTTGCCCAAGTTTGGCGGGATTAAACTCAAAGCAGATGCTAAACCTCAGGAATACCACGCCGCGTTCCGAAACGTGGAGGATCACCTCGACGTCGCTAAAAAACCCCacc                                                   >  2:213925/1‑236 (MQ=255)
                                                                                                                                                                              aaccacAAGGCAAAGCCCACCGACCCCCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAC‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAg                                                    <  1:750085/232‑1 (MQ=255)
                                                                                                                                                                               accGCAAGGCAAATGCACCCGCCCCCCGCCCCCTGCCAGGCGAAATCCGTCAGCAACAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGc                                                   <  1:213288/235‑1 (MQ=255)
                                                                                                                                                                                 cGCCAGGCCAATGCCACCGCCCCCCGCCCCCTGCCAGGCAAAACCCGTCAGCACCAATAAC‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGccccc                                               <  1:430386/239‑1 (MQ=255)
                                                                                                                                                                                 cGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGCGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCCCGCCGAGTTCCGAAAAGAGGGGGGTCCCATACACGTCCCTAAAAAACCCAAGccccc                                               >  2:213288/1‑239 (MQ=255)
                                                                                                                                                                                 cGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCATACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTAAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGATCCGAAAAGCGGAGGAGCTCATCGACGTCGATAATAAACCCCCAcccc                                                >  1:345040/1‑238 (MQ=255)
                                                                                                                                                                                 cGCAAAGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCACTAAT‑CCCGCCAGCAACCCCCCGCTTAACAGCA‑CCTTTGCCGCGCCTACGCCAAAAATCGACCTCCTCAGTTGCCAAAGTTTGCAGGGATCCACCTCAAGGCCGACGATAAACACTCGGAATACCTCCCCGGGTTCCCCAACGGGAGGGATCCCATCCCCACCGCTAAAAAAccaccgaaccc                                               >  2:1127622/1‑230 (MQ=255)
                                                                                                                                                                                  gCAAGGCCAATGCCACCGCCCCCCCCCCCCTGCCAGTCAAAACCCGTCAGCATAAATAAC‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCa                                              <  1:1252060/239‑1 (MQ=255)
                                                                                                                                                                                   cAAGGCCAATCCCACCGACCCCCGCCGCCGGCCAGGCAAAATCCTGCAGCATCAATAAC‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCAc                                             <  1:213925/239‑1 (MQ=255)
                                                                                                                                                                                    aaGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCCACTCAAGGCCGATGATAAACATCAGGAAACCCACGCCGAGTTCCGAAAAGAGGAGGATTCCCCCGACGTCGCCAACAAACCCAAGCCCCCACg                                            >  2:430386/1‑239 (MQ=255)
                                                                                                                                                                                       cccaacccccccGCCCCCCCCCCCCTGCCTGGCAAACTCCCTCAGCCTAAAAAAC‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGc                                         <  2:913652/229‑1 (MQ=255)
                                                                                                                                                                                            tGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAAtt                                    >  1:1179574/1‑239 (MQ=255)
                                                                                                                                                                                                 ccGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGGGTTCCGAAAAGTGGAGAACCTCATCGCCGCCGCAAATAAACCCCACCCCCCACGGCCCAATTGCaa                                >  2:190004/1‑238 (MQ=255)
                                                                                                                                                                                                 ccGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTCCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCaa                                >  1:935678/1‑238 (MQ=255)
                                                                                                                                                                                                  cGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAACGTGGAGGATATCATCGACGTCGCTAATAAACCCCAACACCCACGGGCCAATTGCAAt                               >  1:84339/1‑238 (MQ=255)
                                                                                                                                                                                                    ccccccgccgCCCGCCAGGCGAAACCCGTCAGCATAAATAAC‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATccc                            <  1:449250/235‑1 (MQ=255)
                                                                                                                                                                                                              ccggccagcgcaaaTCCGACAGCATCAAATAC‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCaaa                  <  1:464699/228‑1 (MQ=255)
                                                                                                                                                                                                              cccaccgggCAAAACCCGTAAGCTACAAAAGC‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCGGTGCCGCGCATACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATCCCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCaaa                  <  1:1074367/232‑1 (MQ=255)
                                                                                                                                                                                                              ccTGCCGGGCAAATGCCGCCAAAATCAATAAC‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCaaa                  <  1:66661/239‑1 (MQ=255)
                                                                                                                                                                                                              ccTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGCTAAACATCAGGAATACCACGCCAAGTTCCGAAAACTGGAGGAGCACATCGACGTCACAAATCAACCCCCACCACCACGGGACACATGCAAACCCCGCCACCaaa                  >  2:640201/1‑239 (MQ=255)
                                                                                                                                                                                                              ccTGCCAGGCCAACTCCGCCAGCCTCAATAAC‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCaaa                  <  1:1227873/239‑1 (MQ=255)
                                                                                                                                                                                                              ccTGACAGGCGAAACCCGGCAGCATCAATAAC‑CCCGCAAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGGTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCaaa                  <  1:802850/239‑1 (MQ=255)
                                                                                                                                                                                                                   cagggcaaaTCGGTAAGCATCAAAAAC‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCAAATATcc             <  1:260506/233‑1 (MQ=255)
                                                                                                                                                                                                                      gCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCACAAATCGAACGCCTCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGCTGATAAACATCCGGAATACCACGCCTGGTTCCGCAAATGGGAGCACCCACTCGCCGTCGCCAATAAACCCCCGCCCCCCCCGGCCAACTGCAAACCCCGCGGCCAAAAATCCaaa          >  2:99260/1‑239 (MQ=255)
                                                                                                                                                                                                                      gCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGCGGGATTCAACTCAAGACCGATGCTAAACATCAGGAATACCACGCCCAGTTCCGAAAAGTGGAGGATCTCATCGACGCCGCCAAAAAACCCCCACCCCCACCGGCCAAATGCCATCCACGCCCCCAAACATCCCa           >  2:87665/1‑238 (MQ=255)
                                                                                                                                                                                                                          aaTCCGCAAGCA‑CAATAAACCCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCAAATATCCCAAcaca      <  1:1130584/239‑1 (MQ=255)
                                                                                                                                                                                                                           aTCCGTCAGCTACAAAACC‑CCCGCCAGCAACCCCGCCACTAACAGCC‑CCTGGGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCAAATATCCCAACACAg     <  1:616446/239‑1 (MQ=255)
                                                                                                                                                                                                                              cGTCAGCAACAATAAC‑CCCGCCAGCAACGCCGCGGTTAACAGCC‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCAAATATCCCAACACAgcgc  <  1:692971/239‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                 
ACCTTCATCCAGTCGAAATGTTCGTCTGCCGACCCCGCCAACAACGGCACTAACGCCAGTGCTGGGATTACTGCCAGATCCTGAAACAGCAGAACCGAAAACCCGAGCTGGCCGGATTCGCTGCGATTCATCCCTTTCTCACGCATCA‑ATTGCAACGCCATTGCAGTTGAAGACATCGCAAGGCCAATGCCACCGACCACCGCCGCCTGCCAGGCGAAATCCGTCAGCATCAATAAT‑CCCGCCAGCAACGCCGCGCTTAACAGCA‑CCTGTGCCGCGCCTACGCCAAAAATCGAACGCCGCAGTTGCCAAAGTTTGGAGGGATTCAACTCAAGGCCGATGATAAACATCAGGAATACCACGCCGAGTTCCGAAAAGTGGAGGATCTCATCGACGTCGCTAATAAACCCCAGCCCCCACGGGCCAATTGCAATCCCTGCCAGCAAATATCCCAACACAGCGC  >  NC_000913/3480060‑3480519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A