Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F111 I2 R1
|
16 |
47.6 |
1720206 |
89.7% |
1543024 |
142.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,173,363 |
Δ2 bp |
intergenic (‑1/+1) |
gatC ← / ← gatC |
pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,173,361 | 0 | C | . | 97.5%
| 166.2
/ ‑3.1
| 40 | pseudogene (2/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (16/23); total (16/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 |
| * | NC_000913 | 2,173,362 | 0 | C | . | 97.5%
| 165.6
/ ‑3.3
| 40 | pseudogene (1/442 nt) | gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (16/23); total (16/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
ACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTG > NC_000913/2173227‑2173497
||
aCGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCCGGCTTGCCGATaccaccgccgtat > 1:292558/1‑136 (MQ=255)
tGCATGGCGACCGCCCTCGCCCCGAAGAAGCCGATGGCAGCAAGACCGCCAAACGGCAGCACCTGACGACCCGGCACACAAACAGCCATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTcc < 1:642111/143‑1 (MQ=255)
ccACGGCGACCGCCATCCCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTccc < 1:347212/142‑1 (MQ=255)
aCGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcag > 1:811507/1‑143 (MQ=255)
aCGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCAGGCACACAAACAACAATTAAAATGGTGAGTGGGATAAAAATCAGACTCGCCGCTACCA‑‑CCGCCATATGTACcag > 2:370025/1‑143 (MQ=255)
gcccGCCAGCGCAACGAAGAGGCCGATGGGGGCAAGATCGCCAAACGGCAGCAGCTTATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAgcagca < 1:119041/141‑1 (MQ=255)
cGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACg > 2:730620/1‑143 (MQ=255)
aCGTAGAAGCCGATGGTGGCAAGCCCGCCAAATGGCAGCGGCTGATTACTCGTCTGACACGCAGCAATTAAACTCGTGAGTGGGATAAAAATGAGGCCTGCCGATACCA‑‑CGGCCGTATGTCCCAGCAGCAACGCCGGATCAAg < 2:139520/143‑1 (MQ=255)
aCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAg > 1:698199/1‑143 (MQ=255)
cGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAgg < 2:244159/143‑1 (MQ=255)
acgCCGATGGTGGCCAGATCGCCAAACCCCAGCACCAAATTCCCCGGCCCACAAACAGCAATTAAAATCGCGCGTCGGATAAAAATCAGGCTTGCCTATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAt < 1:418658/141‑1 (MQ=255)
aTGGGGGCAAGATCGCCAAACGGCAGCACCTGATTAGCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAACAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGaa < 2:566213/143‑1 (MQ=255)
tggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTcc > 1:276039/1‑143 (MQ=255)
aGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAcc > 1:687778/1‑143 (MQ=255)
cAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGAACAGAAGGTGCTTTGTCTATCAGGAACTCCTGACCGCCGAAc < 1:817029/143‑1 (MQ=255)
cGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCg < 1:730620/143‑1 (MQ=255)
agcaCCTGATCACCCGCCACACAACCAGCAATTAAAACTGTCAGTGGCATAAAAAACAGGCTTGCCGATACCA‑‑CCGCCGTAAGTCCCAGCCGCACCGCCGGATAACGGCCAATCAGGACCTCCTGACCGCCGAACTTCGCCTg < 1:838520/143‑1 (MQ=255)
gATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGa < 1:509580/143‑1 (MQ=255)
tcaCCCGGCACACAAACAGCAATTAAAATTGTGAGTGGGATAAAAATTAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACt < 2:418377/141‑1 (MQ=255)
tACCCGGCCCACAAACAGCAATTAAGATGGTGAGTGGGATCAACATCAGGCTTGCCGCTACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTa < 2:672268/143‑1 (MQ=255)
aCCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACGGCCGAACTGCGCCTAGACACGACTAc > 2:454273/1‑143 (MQ=255)
aCCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCACAGCACCAACGCCGGATCCAGGCCCATCAGGCACCCCTGACCGCCCAACTTCGCCTGCACACGACTAc > 2:244830/1‑143 (MQ=255)
ccGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTGCCAGCAGCAACGCCGGATCAAGGCCAACCAGGAACACCTGACCGCCGAACTTCGCCTGTAACCGGCTACgc > 2:553127/1‑142 (MQ=255)
cGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAACATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGACCTTCGCCTGTAAACGACTCCgcg > 2:582035/1‑141 (MQ=255)
ggCACACAAACAGCAATTAAAATGGAGAGTGGGATAAACATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCGCAGCAGCAACGCCGGATCAAGGCCAATAAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGCCTACGacc > 2:86767/1‑141 (MQ=255)
aaaCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGGCCCAGCAGCAACGCCGGATCAAGGCCGATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAACCGACCACGAGTGTGCtgg > 1:187849/1‑141 (MQ=255)
ttAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGc > 1:668195/1‑143 (MQ=255)
tcaaaaTGGTGAGCGGGCTAACACTGCGGCGTGCCGCTAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGc < 1:514734/141‑1 (MQ=255)
atAAAAAGGTGAGGGGGATAAAAAACAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGACCAGATAGAGCGGAGGTTTTCGGGTACCCCTAACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGc < 1:396726/142‑1 (MQ=255)
aaaaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGGCTACGAGCCTGCTTAGCGATGGGCGt < 2:208957/143‑1 (MQ=255)
gttaaacaaaaaTCTGGCGGGCCCAAACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatcc < 1:389053/136‑1 (MQ=255)
ggtaTAAAAACCCGGCTCTCCGATACCA‑‑CCGCCTTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatccat < 1:53362/140‑1 (MQ=255)
ggATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCCGTAAACAATCCatg > 1:530040/1‑143 (MQ=255)
agagcgggCTTGGCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAGCGCCGGATCATGGTCAGTCAGGTACTCCCGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGt < 2:410825/137‑1 (MQ=255)
aaaGCAGGGTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTATAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGt < 1:309078/143‑1 (MQ=255)
agtCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGtt < 2:272124/141‑1 (MQ=255)
aaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTAGCCCATAACCAACTACGCGCCTGCCTAGCCATGGGCGTTAACCCATCCACGGTgggca > 2:558962/1‑141 (MQ=255)
tCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTa < 1:701777/143‑1 (MQ=255)
gctGCCGAGACCA‑‑CCGCCGGCTGTGCCGGCGGCTACGCCGGATCAAGGCCAATCAGCAACTCCGGACCGCCGAACTTCGCCTGTAAACGACTACGGGCCTGTTAGGCAATGGTCGGTAAACCATCCATGATGGGTTTAATCAc < 2:62819/141‑1 (MQ=255)
ttGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGACCAAGGCCAACCAGGAACTCCTGACCGCCGACGTTCGCCTGTAAACCACTCCGAGCCTGCTTAGCGATGGGCGTTAAACCATCCACGATAGGTTTGATCaac > 2:684835/1‑141 (MQ=255)
cGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTg < 2:687778/143‑1 (MQ=255)
||
ACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTG > NC_000913/2173227‑2173497
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
