Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F110 I1 R1 18 47.7 1781824 90.5% 1612550 142.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 701,362 G→T S78Y (TCC→TAC)  nagC ← N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913701,3620GT100.0% 121.7 / NA 43S78Y (TCC→TAC) nagCN‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/18);  total (25/18)

TGCTGGGTACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGG  >  NC_000913/701227‑701501
                                                                                                                                        |                                                                                                                                           
tGCTGGGTACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCCCTTTGCTGCTGAGATCAAACAGAGTGCTGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGCAAT‑GGCGGGCTTCGCTGACGCTGTAGATAgc                                                                                                                                      >  2:594393/1‑143 (MQ=255)
    gggTACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGCTGTAGATAGCgagg                                                                                                                                  >  2:212398/1‑143 (MQ=255)
           ttCGGGCAGCGGTTAATGTTTTTCGGCGAGTACTTTGCTGCTGAGATCAACTAGAGTGATGGTGGCGTCATTACGACCAAGCCGTACGCCGATGGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGcccc                                                                                                                           <  2:566889/143‑1 (MQ=255)
                   gCGGGTAATGTTCGTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAg                                                                                                                   <  1:809277/143‑1 (MQ=255)
                    cGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAgg                                                                                                                  <  1:847831/143‑1 (MQ=255)
                     gggTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGc                                                                                                                 <  1:193613/143‑1 (MQ=255)
                       gTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGCTCAAACAGCGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGGGGAAAT‑TGCGGGTTGCGGTGACGACGTAGATAGCGCAGCGGCCCCCGGCGGAGGccc                                                                                                               >  2:736598/1‑142 (MQ=255)
                       gTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCct                                                                                                               >  1:136029/1‑143 (MQ=255)
                        tAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATAAAACAGAGTGATGGTGGCGTCATGACGACGAGGCCGTACGCCGCCTG‑TAGAAAGTATCCCAGCTTTGGTGGCGATGTAGCTGTCGCGGCGGCCCCCGGTGGAGGCctg                                                                                                              >  1:647058/1‑143 (MQ=255)
                          aTGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCctgct                                                                                                            >  1:828580/1‑143 (MQ=255)
                           tGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGTCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGGTGTAGATGGCGCGGCGGCCCCCGGTGGAGGCctgctg                                                                                                           >  1:542688/1‑143 (MQ=255)
                                 tctGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCTATGGCTTGGACAA‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAAc                                                                                                     <  1:242780/143‑1 (MQ=255)
                                    gcgaGCACTTTGCTGCGGAGGTCAAACAGTGTGGTGGTGGCGGCAGGGCGACCAAGCCGTACGCCGATTGCGTGGAAAT‑GGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAActtc                                                                                                  <  2:823323/140‑1 (MQ=255)
                                     ccAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGGCCCCGGTGGAGGCCTGCTGATCAActtct                                                                                                 <  1:846389/143‑1 (MQ=255)
                                      cAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAActtctt                                                                                                >  1:303053/1‑143 (MQ=255)
                                       aGCTCTTTGCTGCTGGGATCGAGCAGGTTCATGTTGGCGTCTTGACGACCTAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCttt                                                                                               <  2:564419/143‑1 (MQ=255)
                                        gCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTg                                                                                              >  1:614292/1‑143 (MQ=255)
                                          aCTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGAt                                                                                            <  1:832790/143‑1 (MQ=255)
                                           cTTTGCTGCTGAGATCAAACAGAGTGATGGTGGGGACATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATc                                                                                           <  1:63130/143‑1 (MQ=255)
                                                 tgctgAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCcg                                                                                     >  1:4778/1‑143 (MQ=255)
                                                     gagaTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGtt                                                                                 >  1:67099/1‑143 (MQ=255)
                                                       gaTCGAACAGGTTGATGGTCGCGTTCTGGCGACCGGGCCGTACGCCGATGGCCTGGAAAT‑TGAGGGTTTCTGTTACGATGTAGATAGCGCGGCGGTTCCCGGTCGAGGCCTGTTGATAAACTTCTTTGATCAGCCCGCGTTCg                                                                               <  2:645986/143‑1 (MQ=255)
                                                        gtCAAACAGAGTGGTGGGGGCGTCATGACGTCCCCGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGa                                                                              <  2:119917/142‑1 (MQ=255)
                                                        aTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAGAT‑TGCGGGGTGCGGCGAGGATGTAGATAGCGCGGCGGGCCCCGGTCGAGGCCTGCGGCGCCAGTTCTTGGAGCAGCGCCCGCTCGa                                                                              >  2:612159/1‑143 (MQ=255)
                                                        aTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGa                                                                              >  1:871799/1‑143 (MQ=255)
                                                        aTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGGTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTGCGa                                                                              >  2:457986/1‑143 (MQ=255)
                                                             aCAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGc                                                                         >  2:362632/1‑143 (MQ=255)
                                                                       ggtggCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTaa                                                               <  1:832665/143‑1 (MQ=255)
                                                                         tggCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAAtt                                                             >  1:630660/1‑143 (MQ=255)
                                                                             gTCATGACCACCAAGCCGTAAGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTgg                                                         <  1:212398/143‑1 (MQ=255)
                                                                                  gacgacCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTCATTTTGCTTCCg                                                    >  1:312464/1‑143 (MQ=255)
                                                                                        cAAGCCGTACGCCGATTGCATGGAAAT‑TGCGGGCTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCCGAGGAGGCCTGCTGATCAACTCCCCTGATCAGCCCCCGGGCGGCAAGCAGACGCATACTTCTGGCTACggccgcc                                              >  2:374379/1‑137 (MQ=255)
                                                                                          tgCCGTAGGCCGATTGGGTGGGCCT‑TGCGGGTTTCGGTGGCGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCggg                                            <  2:293146/142‑1 (MQ=255)
                                                                                                  gCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGCTCAGCCCGCGGTCGGTAAGCTGACGCGTAATTTGGGGTACGCTGGCGGGGGCCAggc                                    >  2:768836/1‑141 (MQ=255)
                                                                                                  gCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGGAAGCt                                    >  1:546852/1‑143 (MQ=255)
                                                                                                      aTTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCt                                <  2:303053/143‑1 (MQ=255)
                                                                                                             ggAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGCCTTTGCTCg                         >  2:737016/1‑143 (MQ=255)
                                                                                                              gAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCCGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTCACGCTGGCGGGGGCAAGCTGGCTTTGCTCgg                        >  2:554827/1‑143 (MQ=255)
                                                                                                                 ct‑tGCGGGTTTCGGTGGCGATGTAGATGGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTTGCTTTGCTCGGCaa                     <  1:85048/142‑1 (MQ=255)
                                                                                                                            tCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTAAATCcg           <  1:888023/143‑1 (MQ=255)
                                                                                                                                   cGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGAtt    >  2:798748/1‑143 (MQ=255)
                                                                                                                                     aTGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATGCGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCACTCTGAATCCGCCAGATTgg  >  2:356873/1‑143 (MQ=255)
                                                                                                                                     aTGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTgg  >  2:70133/1‑143 (MQ=255)
                                                                                                                                        |                                                                                                                                           
TGCTGGGTACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGG  >  NC_000913/701227‑701501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A