Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F110 I2 R1 19 56.9 2036372 91.3% 1859207 142.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,936,308 C→T V3I (GTA→ATA)  zwf ← glucose‑6‑phosphate 1‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,936,3080CT92.5% 114.2 / NA 41V3I (GTA→ATA) zwfglucose‑6‑phosphate 1‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (20/17);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGG  >  NC_000913/1936170‑1936443
                                                                                                                                          |                                                                                                                                       
gtcccccGATAATCCGGGCGTCCCGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGcc                                                                                                                                     <  1:901678/138‑1 (MQ=255)
      cGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCCGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAACGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCa                                                                                                                               <  1:486178/143‑1 (MQ=255)
        aTAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCCAAAATGCCCCGGTCACAGGCCTGGGCTGTTCGCGGTAGAGCCagggaatc                                                                                                                             >  2:431222/1‑136 (MQ=255)
         tAATCCGGGTGTCCGGGTTGACCTGACCGGCTTTTTCCAGTTGATACAGGGAACGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGCCACAGCCCTATTCTGTTTGCGTTATCGCCATGTCATtc                                                                                                                            <  2:888499/143‑1 (MQ=255)
           aTCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAGTTTACCCCGCGCAAGGTCGCCTTTCGCGCCGACCATGACCATGGCAGAGGCCTGGGCTTTTTGCGTTTCCGCCATGTCATtctc                                                                                                                          >  1:616579/1‑143 (MQ=255)
                 gtgtCCGGGTGGACCTGACCCGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTCCGACGCGCAGGGTCTCCTTTCTCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAg                                                                                                                    <  1:446381/143‑1 (MQ=255)
                    tCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAACATGACCAGGTCACAGGCCTGGGCTGTTTGCGCCACCCCCATCTCGTGCTCCCTCAGtaa                                                                                                                 >  2:205431/1‑141 (MQ=255)
                    tCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTta                                                                                                                 >  2:167642/1‑143 (MQ=255)
                      cGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTtaac                                                                                                               <  2:619668/143‑1 (MQ=255)
                      cGGGCTGAGCTGACCGGCTTTTGCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTtaac                                                                                                               <  2:614527/143‑1 (MQ=255)
                       gggTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTTCGTtagct                                                                                                              >  1:795808/1‑143 (MQ=255)
                                    cGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTGTCGCCATGGCATTCTGCTTGAGTTAACTAGCCCGGGACTTa                                                                                                 >  1:475481/1‑143 (MQ=255)
                                       cTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGCCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGc                                                                                              <  1:567970/143‑1 (MQ=255)
                                        tttttCCAGTGGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGcc                                                                                             <  2:285827/143‑1 (MQ=255)
                                         ttttCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCa                                                                                            <  1:996194/143‑1 (MQ=255)
                                            tCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGCACTTAAGCCAggg                                                                                         >  1:412459/1‑143 (MQ=255)
                                                gTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGGTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGtata                                                                                     >  2:819737/1‑143 (MQ=255)
                                                           gAAGGCAGCAATGTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGCTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAAtttt                                                                          <  1:243112/143‑1 (MQ=255)
                                                            aaGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTc                                                                         <  1:167642/143‑1 (MQ=255)
                                                               gcagcaATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCGGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTa                                                                      >  1:235606/1‑143 (MQ=255)
                                                               gcagcaATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGTTATACTTGTAATTTTCTTa                                                                      >  1:90707/1‑143 (MQ=255)
                                                               gcagcaATTTACGACGCGCAAGGTCGCCTCTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTa                                                                      >  1:653395/1‑143 (MQ=255)
                                                                     aTTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAACTGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTACGCCCGGGTATCCTTGTAATTTTCTTACGGTGc                                                                >  2:556431/1‑143 (MQ=255)
                                                                          cgacgCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGt                                                           >  1:125939/1‑143 (MQ=255)
                                                                                     gtcttcTTTCGCGCCGAAAACGACCAGGTCACAGGCCGGGGCTGCCTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTAc                                                <  1:85073/138‑1 (MQ=255)
                                                                                       cGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGCACTACTATTACGa                                              >  2:775/1‑143 (MQ=255)
                                                                                          cTTTCGCGCCGAAAATGACGAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACCTATTTTACGGGCt                                           >  2:201692/1‑143 (MQ=255)
                                                                                              cgcgCCGAAAATGACCACGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCg                                       <  2:243779/143‑1 (MQ=255)
                                                                                                cgcCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGaa                                     >  1:677898/1‑143 (MQ=255)
                                                                                                cgcCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGaa                                     >  2:911926/1‑143 (MQ=255)
                                                                                                   cGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAAc                                  >  1:896618/1‑143 (MQ=255)
                                                                                                    gAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACt                                 <  1:447645/143‑1 (MQ=255)
                                                                                                               ggTCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAACACTGTAACCGCTTa                      >  2:330716/1‑143 (MQ=255)
                                                                                                                 tgacaGGCCTGGGCTGTTTGCGTTATCTCCATGTCATTCTCCTTCTGTTTACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATc                    <  2:875306/141‑1 (MQ=255)
                                                                                                                 tCACAGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATc                    >  2:243710/1‑143 (MQ=255)
                                                                                                                   acaGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTCTACGAGCTTGCGAAAACTCTAAACGCTTATCCa                  >  2:145022/1‑143 (MQ=255)
                                                                                                                    caGGCCTGGGCTGTTTGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTCATCCAc                 <  1:638644/143‑1 (MQ=255)
                                                                                                                      ggCCTGGGCTGTTTGCGTTATCGCCATGTCATGCTCCTTAAGTTAACTAGCCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTCTTACGAGCTTTCGAAAACTGTAAACGCTTATCCAccc               <  2:993412/143‑1 (MQ=255)
                                                                                                                                gtgtGCGTTACCGCCAGGTCATCCGCCATAAGAGAACTAACCCGGTCCTTAAGCCAGGGTATACGTGCAATTTTCTCACGGTCCACCGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTAc     <  1:307472/140‑1 (MQ=255)
                                                                                                                                   tGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAGACTGTAAACGCTTATCCACCCGTGTGATTACggg  >  1:175893/1‑143 (MQ=255)
                                                                                                                                   tGCGTTATCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACggg  >  1:175941/1‑143 (MQ=255)
                                                                                                                                          |                                                                                                                                       
CTACGCCGATAATCCGGGTGTCCGGGTTGAGCTGACCGGCTTTTTCCAGTTGATACAGGGAAGGCAGCAATTTACGACGCGCAAGGTCGCCTTTCGCGCCGAAAATGACCAGGTCACAGGCCTGGGCTGTTTGCGTTACCGCCATGTCATTCTCCTTAAGTTAACTAACCCGGTACTTAAGCCAGGGTATACTTGTAATTTTCTTACGGTGCACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGG  >  NC_000913/1936170‑1936443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A