Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F110 I2 R1
|
16 |
53.0 |
1914758 |
91.2% |
1746259 |
142.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,186,086 |
C→A |
P246Q (CCG→CAG) |
rpoC → |
RNA polymerase, beta prime subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,186,086 | 0 | C | A | 100.0%
| 131.4
/ NA
| 45 | P246Q (CCG→CAG) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (21/24); total (21/24) |
CGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATC > NC_000913/4185953‑4186222
|
cattaaGAGCTGAACGAAAACAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCt < 1:841362/139‑1 (MQ=255)
gTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGCATGATCCTGACCGTTCTGCCGGTACTGCAGCAAGATCTg > 2:90898/1‑143 (MQ=255)
tGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGc < 2:850607/143‑1 (MQ=255)
cTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCt < 1:940575/143‑1 (MQ=255)
aaCGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTATGGTAACAAACCAGAGCGGATGATCCTGACCGTTCTGCCGGTACCGCAGCCAGATCTGCCTACGCTGGt > 2:133632/1‑143 (MQ=255)
aCCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCt < 1:151276/143‑1 (MQ=255)
cgaCTCCGAAGCCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGGAACAAACCCGAGTACTTTATCCTGTCCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTgg < 1:548651/141‑1 (MQ=255)
ccGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGAtggt > 1:925564/1‑143 (MQ=255)
ccGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGGTCCGCTGGAtggt > 1:173437/1‑143 (MQ=255)
gAAACCAAGCGTAAAAAGCTGACCAATCGTATCAAACTGCTAGAAGAATTCGTTAAGTCTGGTAACAAACAAGAGTGGATGATCCTGACCGTCCTGCAGGAACTGCAGCCAGATCTCCGTCCCCGCGTTCCTCTGGAtgcggg > 2:67028/1‑139 (MQ=255)
aaaCCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATggtggt > 1:674206/1‑143 (MQ=255)
cGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTcgc < 1:430231/143‑1 (MQ=255)
tAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGa > 1:240028/1‑143 (MQ=255)
tAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGa < 1:799834/143‑1 (MQ=255)
gaaaaaGCTGACCAAGCGTATCACACTGCTGGAAGCGTTCTTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCACAGCTTCTTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGa < 2:291918/142‑1 (MQ=255)
cTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGa < 1:50889/143‑1 (MQ=255)
cTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTGCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGa < 1:513946/143‑1 (MQ=255)
gACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGCCCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGAcc > 1:209482/1‑143 (MQ=255)
aCCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGGTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGAGACTTGTGGCCt > 2:301872/1‑143 (MQ=255)
cAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCGGa > 1:921867/1‑143 (MQ=255)
aaGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCCGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGAGGGTGGTCGTTCCGCGACTTCTGGccatga > 2:88599/1‑139 (MQ=255)
tCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGCTGGTGGTCGTTTCGCGAATTCTGACCTGAACGATCTg > 2:750568/1‑143 (MQ=255)
cAAACTGCCGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGt < 1:92228/143‑1 (MQ=255)
tgGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTcgc < 1:545799/143‑1 (MQ=255)
ggAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTcgcg < 2:407192/143‑1 (MQ=255)
aGCGTTCTTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCGGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCa < 1:940115/143‑1 (MQ=255)
cgttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAAcc < 1:335798/143‑1 (MQ=255)
gttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGCCCTGCACGATCTGTATCGTCGCGTCATGAACCg > 2:235127/1‑143 (MQ=255)
ttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGt > 1:621534/1‑143 (MQ=255)
cattCTGGTAACAAGTTAGAGTGGTTGATGCTGATCGTTCTGCCGGTACTGCAGCCAGATCTGCGTTCGTTTGTTCTGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTaa < 2:541076/140‑1 (MQ=255)
gTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGAAACAAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCATTTCGCGACTTCTGACCTGAACGATCTGTACCGGCACGTCATGAAcctgagaa > 2:99017/1‑137 (MQ=255)
tCTGGTAACGAGCCTGAGTGGATGAGCCTGACCGTTCTGCGGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTaacaa < 2:300557/143‑1 (MQ=255)
gTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGt > 1:826416/1‑143 (MQ=255)
aCCGGAGTGGATGATCCGGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAAc < 1:492157/143‑1 (MQ=255)
aCCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAAc < 1:722455/143‑1 (MQ=255)
cAGAGTGGATGTGCTTGACCGGTCTGCCGTTACTGCAGCCAGAGCTGCGTCCGCGGGTTCCGCTGGATGGTGGTCGGTTCGCGAGTTCTGACCGGAGCGATCTGTATCGTCGCGTCATTAACCGTGACAACCGTCTGAAACGt < 2:6187/143‑1 (MQ=255)
agTGGATGATCCTGTCCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTctg < 2:393375/143‑1 (MQ=255)
tgatCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCAACTTCTGACCTGCAGGATCTGTATCGTCGCCTCCTTAAACGTAACAACCGTCTGAAACGTCTGCAGGaa > 2:61804/1‑142 (MQ=255)
gatCCTGACCGTTCTGCCGGTACTGCAGCCAGAACTGCGTCCGCTGGTTCCGCTGGATGGTGATCGTTTCGCGACTTCTGACCTTAACGATCTGTCTCGTCGCGTCATTAACCGTCACAAGCGTTTGCAACGTCTGCTGGATc > 1:631030/1‑143 (MQ=255)
cTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAATGATCTGTATCGTCGCGTCATTAACCGAAACAACCGTCTGAAACGTCTGCGGGAttttgc > 1:671143/1‑138 (MQ=255)
ttCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCg < 2:842999/143‑1 (MQ=255)
cggCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGa < 1:525575/141‑1 (MQ=255)
gCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCACGACTTCTGACCTGAACGATCTGTATCGTCGAGTCATTAACCGTAACAACAGTCTGAAACGTATGCTGTGTCTTAATGAGCCGGAca > 2:727825/1‑143 (MQ=255)
ggTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGCACGCCCTGTATCGTCGCGTCATTCACTGAAAAAATCGTCCGAACCGACTGCTGGCTCTGGCTGCGCCGGAcatca > 2:567280/1‑143 (MQ=255)
tACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTCTCGCTACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACatcatc < 2:674206/143‑1 (MQ=255)
|
CGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATC > NC_000913/4185953‑4186222
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
