Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F2000 I1 R1	34	165.3	3515406	94.0%	3304481	221.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007779_cfb	2,382,028	C→A	D79Y (GAC→TAC)	menH ←	peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779_cfb	2,382,028	0	C	A	98.4%	396.0 / ‑1.6	123	coding (235/759 nt)	menH	peptidase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (64/57); minor base . (1/1); total (65/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 126 total aligned reads displayed.

CGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCT  >  NC_007779_cfb/2381788‑2382270

cGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGGCCGGTATGGGTGCCATCCGCAGGTTGTTCAGTATTTTTCAGCCCCGCGTCGCCGCCGTCGACAATAA‑ACCCCCCAAA‑CCCCGCCCGCCCCTTGCAAACCGCCATCATCGCCACCCCTCCACCACACTAGCACCCCACCACCCCGCAGTAAAAGAtgct                                                                                                                                                                                                                                            >  2:130979/1‑248 (MQ=255)

            agGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCaa                                                                                                                                                                                                                                >  2:395872/1‑250 (MQ=255)

                              gATCCCAGTGGACAAATCCCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTc                                                                                                                                                                                                             <  1:744983/250‑1 (MQ=255)

                              gATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGCAAAGGATGTTGTAACTAACCAAGG‑TTTTCCGAAGTaattg                                                                                                                                                                                                             >  2:1311112/1‑247 (MQ=255)

                                 aCCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGt                                                                                                                                                                                                          >  1:52980/1‑250 (MQ=255)

                                  ccAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTg                                                                                                                                                                                                         <  1:600955/250‑1 (MQ=255)

                                    cccgatgaatacccgccgGTAACGGTTCTTGGAGAAAACGCTGCACCCATGGGCGCCCGGAACGCTGACGTTCCGCACTTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGAc                                                                                                                                                                                                       <  2:294088/238‑1 (MQ=255)

                                     gTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGAca                                                                                                                                                                                                      >  2:144728/1‑250 (MQ=255)

                                                  gccgTTAACGGTTCCGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCAt                                                                                                                                                                                         <  1:1742601/250‑1 (MQ=255)

                                                   ccgTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGt                                                                                                                                                                                                          >  2:311124/1‑232 (MQ=255)

                                                   ccgTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGt                                                                                                                                                                                                          <  1:311124/232‑1 (MQ=255)

                                                    ctttAACGGTTCGGTGAGAAACCGCTGCACCCATTGGCGGTCGGATCGCTGAGTTTCCGAACGTTGTTCAGCATTTTGCAGCCCCGGAGGCCCGCCTTCGACAATAC‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCg                                                                                                                                                                                       <  2:273323/248‑1 (MQ=255)

                                                    cgTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCg                                                                                                                                                                                       <  1:144728/250‑1 (MQ=255)

                                                     gTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAg                                                                                                                                                                                                               >  2:1147546/1‑225 (MQ=255)

                                                      ttAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGAc                                                                                                                                                                                     >  1:639788/1‑250 (MQ=255)

                                                              tCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCACCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCaa                                                                                                                                                                                                                                >  2:1703852/1‑200 (MQ=255)

                                                              tCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCACCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCaa                                                                                                                                                                                                                                <  1:1703852/200‑1 (MQ=255)

                                                               cTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAAt                                                                                                                                                                                             <  1:212508/233‑1 (MQ=255)

                                                                tgtgAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATcgcc                                                                                                                                                                           <  1:1028276/250‑1 (MQ=255)

                                                                 gtgAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCg                                                                                                                                                                                       <  1:1533099/237‑1 (MQ=255)

                                                                 gtgAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCg                                                                                                                                                                                       >  2:1533099/1‑237 (MQ=255)

                                                                      aaaaCGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACg                                                                                                                                                                                                                      >  2:1391442/1‑201 (MQ=255)

                                                                           gCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAaccacc                                                                                                                                                                >  1:1491505/1‑250 (MQ=255)

                                                                                 cccATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGAcc                                                                                                                                                          >  1:1736750/1‑250 (MQ=255)

                                                                                              ggAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCCCCCGTCCACCAAGCGAGCACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACa                                                                                                                                             >  1:990285/1‑250 (MQ=255)

                                                                                                    cTGACGTTCCGCACGTTGTTCAGCATTTTGCATCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑TCCCGCCAGCCCCTGGCAAGCCGCTATCATCGCCACCCGTCCACCAAGCGAGGACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAAc                                                                                                                                       >  1:1164084/1‑250 (MQ=255)

                                                                                                     tGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTg                                                                                                                                                        <  1:735413/232‑1 (MQ=255)

                                                                                                     tGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTg                                                                                                                                                        >  2:735413/1‑232 (MQ=255)

                                                                                                         gTTCCGCACGTTGTTCAGCATTTTGCAGCCCGGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGa                                                                                                                                  >  2:596577/1‑250 (MQ=255)

                                                                                                                         aGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGcc                                                                                                                  >  1:754872/1‑250 (MQ=255)

                                                                                                                             ttttGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTgg                                                                                                                                                       <  1:666724/209‑1 (MQ=255)

                                                                                                                                  cccccccgtgaccccGCCTTCCAAAAACACCCCCACAAGC‑CCCCGCAAGCCCTTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCGCCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCAc                                                                                                          <  2:800342/238‑1 (MQ=255)

                                                                                                                                   aGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑ttt                                                                                                                                                                                                                          <  1:95511/136‑1 (MQ=255)

                                                                                                                                   aGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑ttt                                                                                                                                                                                                                          >  2:95511/1‑136 (MQ=255)

                                                                                                                                   aGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCActt                                                                                                        >  2:348601/1‑250 (MQ=255)

                                                                                                                                      cccGGATGCCCGCCTTCGACAATAC‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCActtctt                                                                                                     <  1:1714184/250‑1 (MQ=255)

                                                                                                                                      cccGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCActtctt                                                                                                     >  2:1399940/1‑250 (MQ=255)

                                                                                                                                       ccGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTc                                                                                                                                                                                                             >  2:834941/1‑145 (MQ=255)

                                                                                                                                       ccGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTc                                                                                                                                                                                                             <  1:834941/145‑1 (MQ=255)

                                                                                                                                          gATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTc                                                                                                                >  2:195118/1‑235 (MQ=255)

                                                                                                                                                cGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTg                                                                                           <  1:395872/250‑1 (MQ=255)

                                                                                                                                                  ccTTCGACAATAA‑CCGCACAAAG‑CCCCGCCAGCCCCCGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTCACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCCCTTCTTGCCATTCGttcc                                                                                         >  2:1493497/1‑247 (MQ=255)

                                                                                                                                                   cTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTATCCAAGGTTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGc                                                                                         >  1:161934/1‑250 (MQ=255)

                                                                                                                                                     tCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCACGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGCGATCAACATACAACCGTGAGTAGTCGGCAGACGCCTCGCCCACTTCTTCCCATTCGTNGCnan                                                                                      >  2:976125/1‑249 (MQ=255)

                                                                                                                                                      acgcAATAA‑CCCCACAAAGCCCCCCCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAAt                                                                                      <  2:52980/247‑1 (MQ=255)

                                                                                                                                                           aTAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGcc                                                                                                  <  1:751895/232‑1 (MQ=255)

                                                                                                                                                           aTAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGcc                                                                                                  >  2:751895/1‑232 (MQ=255)

                                                                                                                                                             aa‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGaa                                                                              <  1:1311112/250‑1 (MQ=255)

                                                                                                                                                              a‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGagt                                                                                                                                <  1:1206736/199‑1 (MQ=255)

                                                                                                                                                              a‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGagt                                                                                                                                >  2:1206736/1‑199 (MQ=255)

                                                                                                                                                               cccccACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGaaa                                                                             <  1:130979/249‑1 (MQ=255)

                                                                                                                                                                 cccACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGa                                                                                                                                                            >  1:252916/1‑169 (MQ=255)

                                                                                                                                                                 cccACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGa                                                                                                                                                            <  2:252916/169‑1 (MQ=255)

                                                                                                                                                                 cccACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGaaaaa                                                                           >  1:329303/1‑250 (MQ=255)

                                                                                                                                                                    aaaaaG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAAt                                                                                                                                                                                             <  2:748210/131‑1 (MQ=255)

                                                                                                                                                                        aG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCATCATACAAc                                                                                                                                       <  1:984265/183‑1 (MQ=255)

                                                                                                                                                                         g‑CCCCGCCAGCCCCTAGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCaa                                                                   <  1:924273/250‑1 (MQ=255)

                                                                                                                                                                           ccccGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACACTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACGTACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCaaa                                                                  >  1:1083741/1‑250 (MQ=255)

                                                                                                                                                                               gCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCActtc                                                                                                       >  2:434946/1‑209 (MQ=255)

                                                                                                                                                                               gCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCActtc                                                                                                       <  1:434946/209‑1 (MQ=255)

                                                                                                                                                                               gCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGACCACCAAGCGAGTACCCCACCAGCCACAAGT‑AAGGATGTTGTAACTACCAAAGG‑TTTTACGCCGTAATTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCAAACCACCGTGACCTGTGAGATCAACATACAACCGCGAGGAGTCGGCAAACGCCTCGCCCACTTCTTGCCCCACGTGGCAATCGCCGGACAAACCGTGccagaccac                                                             >  2:1286690/1‑242 (MQ=255)

                                                                                                                                                                                ccAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAacac                                                             <  1:282281/250‑1 (MQ=255)

                                                                                                                                                                                 cAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACAcc                                                            >  1:16840/1‑250 (MQ=255)

                                                                                                                                                                                  aGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCACTCGCCGGAACAACCGTGCAAAAccacaa                                                           >  2:97657/1‑248 (MQ=255)

                                                                                                                                                                                  aGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGGAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCaaagatcgga                                                           >  1:1550612/1‑243 (MQ=255)

                                                                                                                                                                                   gCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCa                                                                    <  1:1417518/240‑1 (MQ=255)

                                                                                                                                                                                     ccctctcatgcctccATCATCCCCACCCTTCCTCCAACCGACTACCCCAGCAGCCAGAAGTAACGTATGTTGTAACTAACCTAGG‑TTTTACGCAGTCAGTCGGTTGCCTCATCGAATCCATCGACGCTAATCGCCGCCGAACCTCCGTGGCCTTCGAGATCAACATACACCCGTGAGTTGTCGGCAAACGCCTCGCCCACTACTTGCCATTCGTGGCGACCGCCGGAAAAACCGTGCAAAATCAccagcc                                                        <  2:455959/241‑1 (MQ=255)

                                                                                                                                                                                     cccTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACGACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagcc                                                        >  2:462677/1‑250 (MQ=255)

                                                                                                                                                                                      ccTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACAccagcca                                                       >  1:738751/1‑250 (MQ=255)

                                                                                                                                                                                            aaGCCCCCATCATCGCCACCCGCCCACCAAGCGAGTACCCCACCAGCCAGCAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTCCGCCGTAAGTCGGCGACATCATCAAATCCATCGACGCTAATCGCCGCCGACCCACCGTGACCTGGGCGATCAACATACAAACGTGAGTCGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCACTCGCGTGAAAAACCGGGCAAAAACACCAGCCAGAGTaa                                                 >  2:797968/1‑250 (MQ=255)

                                                                                                                                                                                              gccgccATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCATCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAAc                                               <  2:1736750/250‑1 (MQ=255)

                                                                                                                                                                                                  ccATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATcg                                                                                                                                                                             >  1:1030350/1‑120 (MQ=255)

                                                                                                                                                                                                  ccATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACGACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTgg                                           <  1:462677/250‑1 (MQ=255)

                                                                                                                                                                                                  ccATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGAAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATcg                                                                                                                                                                             <  2:1030350/120‑1 (MQ=255)

                                                                                                                                                                                                   catcatCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTg                                                                                                                                                        >  1:1246197/1‑140 (MQ=255)

                                                                                                                                                                                                   catcatCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTg                                                                                                                                                        <  2:1246197/140‑1 (MQ=255)

                                                                                                                                                                                                   catcatCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCa                                                                                                                                                <  2:1175428/148‑1 (MQ=255)

                                                                                                                                                                                                   catcatCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCa                                                                                                                                                >  1:1175428/1‑148 (MQ=255)

                                                                                                                                                                                                         cGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCg                                    >  2:1498164/1‑250 (MQ=255)

                                                                                                                                                                                                            ccgaacnccaccaAGCGATTACCCCCCCAACCAGAAAGAAAGGATGTTGTAACTAACCAAGG‑TTGTACGCAGTAAGTCGGTGACTTCATCAAATGCATCGACGCTAAGCGCCGCCGAACCGCCGTGACCTGGGAGATCAACATACAACCGTGAGAACTCGAGAAACGCCTCGCCCGCTTCTTAACATTCGTGGCAAGCGCCGGAAAAACCGTGCAGAAACACCAGCCAGGGTAAACCTGGTTTTCCgtgt                                 <  2:1370674/243‑1 (MQ=255)

                                                                                                                                                                                                            cACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCgtgt                                 >  1:37270/1‑250 (MQ=255)

                                                                                                                                                                                                               ccGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTggg                                                                                                                                                      >  1:664225/1‑130 (MQ=255)

                                                                                                                                                                                                               ccGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTggg                                                                                                                                                      <  2:664225/130‑1 (MQ=255)

                                                                                                                                                                                                                 gTCCACCAAGCGAGTACCCCACCAGCCAGAAGTAAAGGAAGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGACGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGCGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGACCACTTCTTGCTATTCGTGGCAATCGCCGGAAAAACCGTGACAAAACACCAGCAAGGGGAAACCTGGTTTTCCGTGTTGTGc                            >  2:695024/1‑250 (MQ=255)

                                                                                                                                                                                                                   ccaccaAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATCCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCt                          <  2:639788/250‑1 (MQ=255)

                                                                                                                                                                                                                   ccaccaAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑CTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAACCCTGGTTTTCCgtgtagatcgg                          >  1:1370674/1‑243 (MQ=255)

                                                                                                                                                                                                                      ccaAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGaaa                                                                             <  2:774760/196‑1 (MQ=255)

                                                                                                                                                                                                                      ccaAGCGAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGaaa                                                                             >  1:774760/1‑196 (MQ=255)

                                                                                                                                                                                                                            gAGTACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCa                 <  1:695024/250‑1 (MQ=255)

                                                                                                                                                                                                                               tACCCCACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTcc                                     >  1:1347711/1‑227 (MQ=255)

                                                                                                                                                                                                                                 ccccACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGttt                                        <  1:410695/222‑1 (MQ=255)

                                                                                                                                                                                                                                 ccccACCAGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGttt                                        >  2:410695/1‑222 (MQ=255)

                                                                                                                                                                                                                                   ccaccaGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCAt          <  1:759149/250‑1 (MQ=255)

                                                                                                                                                                                                                                     accaGCCAGAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACACTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACGTACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATaa        <  2:1083741/250‑1 (MQ=255)

                                                                                                                                                                                                                                      ccagccagAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATaaa       <  1:1498164/250‑1 (MQ=255)

                                                                                                                                                                                                                                       cagccagAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCGGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAAt      <  2:16840/250‑1 (MQ=255)

                                                                                                                                                                                                                                         gccagAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCg                                                                                    >  1:1030828/1‑170 (MQ=255)

                                                                                                                                                                                                                                         gccagAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTAGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCg                                                                                    <  2:1030828/170‑1 (MQ=255)

                                                                                                                                                                                                                                          ccagAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGc   >  2:261667/1‑250 (MQ=255)

                                                                                                                                                                                                                                           cagAAGTAAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCt  >  2:1348939/1‑250 (MQ=255)

CGTCATTGAGTGAGGCAAAAACAGGCTGTTGATACCAGTCGGCAAATACCGCCGTTAACGGTTCTGTGAGAAAACGCTGCACCCATTGGCGATCGGAACGCTGACGTTCCGCACGTTGTTCAGCATTTTGCAGCCCCGGATGCCCGCCTTCGACAATAA‑CCCCACAAAG‑CCCCGCCAGCCCCTGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGG‑TTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAACCTGGTTTTCCGTGTTTTGCCTGCGCGTGCAGGATCATAAATGGCT  >  NC_007779_cfb/2381788‑2382270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A