Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A69 F2000 I2 R1 32 130.0 2789900 93.6% 2611346 220.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007779_cfb 547,835 +G intergenic (+264/‑1015) fdrA → / → ylbF acyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007779_cfb547,8311.G93.0% 225.6 / 11.2 86intergenic (+260/‑1019)fdrA/ylbFacyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (3/3);  new base G (38/42);  total (41/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

GTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑A‑‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCT  >  NC_007779_cfb/547583‑548067
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              
gTTTACATCAGTGGCTCAAGCCGATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑A‑‑g                                                                                                                                                                                                                                              >  2:1200189/1‑250 (MQ=255)
gTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCATCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑A‑‑g                                                                                                                                                                                                                                              <  1:471918/250‑1 (MQ=255)
  ttACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCTGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑A‑‑ggg                                                                                                                                                                                                                                            >  2:528375/1‑250 (MQ=255)
   tACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAGCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACAGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑A‑‑ggg‑g                                                                                                                                                                                                                                          <  2:15443/250‑1 (MQ=255)
   tACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑A‑‑ggg‑g                                                                                                                                                                                                                                          >  1:1118480/1‑250 (MQ=255)
    aCATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑A‑‑ggg‑gg                                                                                                                                                                                                                                         >  1:378142/1‑249 (MQ=255)
    aCATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑A‑‑ggg‑gg                                                                                                                                                                                                                                         <  2:1254493/250‑2 (MQ=255)
    aCATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTCCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑A‑‑ggg‑gg                                                                                                                                                                                                                                         >  2:1347191/1‑249 (MQ=255)
       tCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGCGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGCGCGCATGTCTGTTCGAAGGGTGGGCGAAAGATGAAGCGCAGGCGCTGGCAACACTGGAG‑C‑A‑‑ggg‑ggaag                                                                                                                                                                                                                                      >  2:221741/1‑246 (MQ=255)
                 aaGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCAt                                                                                                                                                                                                                             >  1:1393306/1‑250 (MQ=255)
                    ccAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTcc                                                                                                                                                                                                                          <  2:1118480/250‑1 (MQ=255)
                      gaTGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCtt                                                                                                                                                                                                                        <  1:1200189/249‑1 (MQ=255)
                              ggTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACCTCATTCCTTGTCACCAt                                                                                                                                                                                                                <  2:378142/250‑1 (MQ=255)
                               gTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACTCTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCAtg                                                                                                                                                                                                               >  1:315863/1‑250 (MQ=255)
                                 ttcggANCAAATTCGTCGCGTTCGTCCACACTGGCTGGATGTACAACCGCATTGTGCACTTATCAGCGAGCTAAACGAGGGTAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGCTGACCGGACCCATGAAAGGGGCGTGCGTAGGCGCATGTCTGTTCGAAGGTTGGGTGAAAGATGAAGCGCAGTCTCTGGCAATACTGGAG‑T‑A‑‑GGGCGAAGTGATCATCATTCCTTGTCTCAAtgtg                                                                                                                                                                                                             <  2:1117637/246‑1 (MQ=255)
                                     gAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATg                                                                                                                                                                                                         >  2:827196/1‑250 (MQ=255)
                                         aaaTTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGt                                                                                                                                                                                                     >  2:980019/1‑250 (MQ=255)
                                          aaTTCGTCGCGCTCGTCCACACTTGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCTAACTAAACGAGGGCAGAACACTGCATCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGGAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc                                                                                                                                                                                                    <  2:66184/250‑1 (MQ=255)
                                              cgtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCCTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc                                                                                                                                                                                                >  1:49753/1‑250 (MQ=255)
                                                    gcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTg                                                                                                                                                                                                                                     >  1:1157670/1‑207 (MQ=255)
                                                    gcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTg                                                                                                                                                                                                                                     <  2:1157670/207‑1 (MQ=255)
                                                        gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg                                                                                                                                                                                      >  2:1334869/1‑250 (MQ=255)
                                                         tCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGt                                                                                                                                                                                     <  1:827196/250‑1 (MQ=255)
                                                               ctggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGGGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGTCAATGGGCGGTATTACt                                                                                                                                                                               <  2:1393306/250‑1 (MQ=255)
                                                               ctggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACt                                                                                                                                                                               <  2:321417/250‑1 (MQ=255)
                                                                tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAGCTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACtt                                                                                                                                                                              >  1:119813/1‑250 (MQ=255)
                                                                tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCa                                                                                                                                                                                                                    >  1:724041/1‑212 (MQ=255)
                                                                tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCa                                                                                                                                                                                                                    <  2:724041/212‑1 (MQ=255)
                                                                tggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACtt                                                                                                                                                                              <  1:1334869/250‑1 (MQ=255)
                                                                 ggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCg                                                                                                                                                                                                      >  1:1036358/1‑225 (MQ=255)
                                                                 ggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCAGTCCTTGTCACCATGTGAATGCCg                                                                                                                                                                                                      <  2:1036358/225‑1 (MQ=255)
                                                                  gctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCGGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCt                                                                                                                                                                            <  1:1114415/250‑1 (MQ=255)
                                                                  gctgaATGTGCAACCGGCCTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCt                                                                                                                                                                            <  2:49753/250‑1 (MQ=255)
                                                                       aTGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAg                                                                                                                                                                       >  1:565655/1‑250 (MQ=255)
                                                                               ccGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGt                                                                                                                                                                                                     >  2:337147/1‑212 (MQ=255)
                                                                               ccGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGt                                                                                                                                                                                                     <  1:337147/212‑1 (MQ=255)
                                                                                   cttcttCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATg                                                                                                                                                                   >  2:683398/1‑242 (MQ=255)
                                                                                   cttcttCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGAAGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATg                                                                                                                                                                   <  1:683398/242‑1 (MQ=255)
                                                                                    ttcttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCAc                                                                                                                                                                                                                   >  2:656952/1‑193 (MQ=255)
                                                                                    ttcttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCAc                                                                                                                                                                                                                   <  1:656952/193‑1 (MQ=255)
                                                                                                   ccaaCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAACGCGCTGGCAGGAGATGACCGGACCCATGCAAGGGGCGTGCGTGCGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCGGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGnagtgn                                                                                                                                           >  2:799158/3‑246 (MQ=255)
                                                                                                   cGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTc                                                                                                                                                                                                                     >  2:95702/1‑176 (MQ=255)
                                                                                                   cGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTc                                                                                                                                                                                                                     <  1:95702/176‑1 (MQ=255)
                                                                                                    gAACTAAACGAGGGCAAAACACTGCTTCACGCCTGGCCGCCAATGCGCTGGCAGGAGATGACCGGATCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGTTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAAGGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGCGGTTGAGAActgga                                                                                                                                          >  2:1224640/1‑246 (MQ=255)
                                                                                                    gAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGa                                                                                                                                          >  1:686340/1‑250 (MQ=255)
                                                                                                     aaCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCATCGAGCCAATGGGCGGTATTCCTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGAc                                                                                                                                         <  1:1224640/250‑1 (MQ=255)
                                                                                                       cTAAACGAGGGTAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCTGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCg                                                                                                                                       >  2:1300525/1‑250 (MQ=255)
                                                                                                         aaaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAGTGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGAc                                                                                                                                     <  2:1059973/250‑1 (MQ=255)
                                                                                                          aaCGAGGGNAAAACACTGGGTAGCGCGGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCAGGAAAGGGGCGTGCGTGGGCGCGTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCGCCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACg                                                                                                                                    <  2:686340/250‑1 (MQ=255)
                                                                                                              agggCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc                                                                                                                                                                                                <  2:845072/184‑1 (MQ=255)
                                                                                                              agggCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGc                                                                                                                                                                                                >  1:845072/3‑186 (MQ=255)
                                                                                                                ggCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATggg                                                                                                                                                                                         >  1:200955/1‑191 (MQ=255)
                                                                                                                ggCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATggg                                                                                                                                                                                         <  2:200955/191‑1 (MQ=255)
                                                                                                                 gCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCg                                                                                                                             <  2:623918/250‑1 (MQ=255)
                                                                                                                   aaaaCACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑A‑‑ggg‑ggaa                                                                                                                                                                                                                                       <  2:297476/141‑4 (MQ=255)
                                                                                                                   aaaaCACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑A‑‑ggg‑ggaa                                                                                                                                                                                                                                       >  1:297476/1‑138 (MQ=255)
                                                                                                                     aaCACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CGG‑‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCg                                                                                                                         >  1:368672/1‑250 (MQ=255)
                                                                                                                      acacTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGt                                                                                                                        >  1:747874/1‑250 (MQ=255)
                                                                                                                             ttCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCaa                                                                                                                 >  1:450056/1‑250 (MQ=255)
                                                                                                                                  gCCGGGCCGCCAATGCGCTGGCAGGAGGTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCa                                                                                                            >  1:221802/1‑250 (MQ=255)
                                                                                                                                  gCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGTCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCa                                                                                                            <  1:1347191/250‑1 (MQ=255)
                                                                                                                                   ccGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCA‑GCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTC‑TTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACg                                                                                                         <  1:762326/250‑1 (MQ=255)
                                                                                                                                       gccgccAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGaa                                                                                                       <  2:463611/250‑1 (MQ=255)
                                                                                                                                           ccAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTa                                                                                                   <  1:980019/250‑1 (MQ=255)
                                                                                                                                                         ggAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATg                                                                                     >  1:991175/1‑250 (MQ=255)
                                                                                                                                                          gagaTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑CGG‑‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGc                                                                                    <  2:368672/250‑1 (MQ=255)
                                                                                                                                                               gACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGtttt                                                                               <  2:315863/250‑1 (MQ=255)
                                                                                                                                                                aCCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTg                                                                              <  1:799158/250‑1 (MQ=255)
                                                                                                                                                                    gACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTT‑GGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATc                                                                                                 >  2:167082/1‑226 (MQ=255)
                                                                                                                                                                    gACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTT‑GGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATc                                                                                                 <  1:167082/226‑1 (MQ=255)
                                                                                                                                                                      cccATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCtt                                                                        >  1:1392193/1‑250 (MQ=255)
                                                                                                                                                                         aTGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCTGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACg                                                                     <  1:1300525/250‑1 (MQ=255)
                                                                                                                                                                         aTGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACgg                                                                                                                                   >  1:691092/1‑188 (MQ=255)
                                                                                                                                                                         aTGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACgg                                                                                                                                   <  2:691092/188‑1 (MQ=255)
                                                                                                                                                                         aTGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACg                                                                     <  2:221802/250‑1 (MQ=255)
                                                                                                                                                                                gggCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AGGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCggg                                                                                                                           >  2:320995/1‑190 (MQ=255)
                                                                                                                                                                                gggCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AGGGGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCggg                                                                                                                           <  1:320995/190‑1 (MQ=255)
                                                                                                                                                                                    gtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑A‑‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGt                                                                                  >  1:574294/1‑225 (MQ=255)
                                                                                                                                                                                    gtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑A‑‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGt                                                                                  <  2:574294/225‑1 (MQ=255)
                                                                                                                                                                                     tcgatgagcgcATGTCTTGTCGAAGGTTTGGCAAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAGCTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTcc                                                         <  2:119813/243‑1 (MQ=255)
                                                                                                                                                                                        gtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGa                                                      >  2:1028552/1‑250 (MQ=255)
                                                                                                                                                                                              gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCCGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGc                                                                                                                          <  1:682155/176‑1 (MQ=255)
                                                                                                                                                                                              gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCCGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGc                                                                                                                          >  2:682155/1‑176 (MQ=255)
                                                                                                                                                                                                                tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCAACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGAt                               <  2:48519/249‑1 (MQ=255)
                                                                                                                                                                                                                tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCAACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGAt                               >  1:48519/1‑249 (MQ=255)
                                                                                                                                                                                                                      aaaGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTaa                        >  2:1270581/1‑250 (MQ=255)
                                                                                                                                                                                                                      aaaGAAGAAGCGAAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGAGAACTTCATTCATCGTAACCATGTGAATGCCGTCGGGTCAATCGGCGGTATTACTTCTGCCTGTATGCCGATGCTGGTGGTTGAGAACGTGAACTTTGGCAACCGGGCGTATTGCAACCTCAACGTAGGTATCGGCATAGTGATGCGTTCTGGCGCTACCCGCGAAGATGTCCTGCCTCCCCCTTCCTTGATGCGCGAGNNGntca                        >  2:971172/1‑248 (MQ=255)
                                                                                                                                                                                                                       aaGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAAt                       <  1:528375/250‑1 (MQ=255)
                                                                                                                                                                                                                        aGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATg                      <  2:747874/250‑1 (MQ=255)
                                                                                                                                                                                                                        aGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCAATGTGTTAATg                      <  2:450056/250‑1 (MQ=255)
                                                                                                                                                                                                                          aTGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGcc                    >  1:300501/1‑250 (MQ=255)
                                                                                                                                                                                                                                cgcAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGAATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGCTGCGCGATGTGTTAATGCAAGTAtt              >  1:721434/1‑250 (MQ=255)
                                                                                                                                                                                                                                 gcAGGCGCTGGCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGT‑ACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCt                                          <  1:917655/220‑1 (MQ=255)
                                                                                                                                                                                                                                 gcAGGCGCTGGCAATACTGCAGCC‑G‑‑GGG‑GAAGTGAACTTCATTCCTTGT‑ACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCt                                          >  2:917655/1‑220 (MQ=255)
                                                                                                                                                                                                                                           gCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGc                                                                                                                   <  1:199959/138‑1 (MQ=255)
                                                                                                                                                                                                                                           gCAATACTGGAG‑C‑AG‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGc                                                                                                                   >  2:199959/1‑138 (MQ=255)
                                                                                                                                                                                                                                            cAATACTGGAG‑C‑AG‑GGG‑GAAGCGATGTTCATTCCTTGTCCCCCTGTGAAGGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGCGACCGGCGGAAACCGGGCGTACTGCAACCTCAACGAAGGAATCGGCAAAGTTATGCGTTTTGACTGTTACGGCGAAGATGTCCTGACTTGCCATCGCTTGCTTTTCGATGTGTTAATGCTCGTTTTAAgctctccgct  >  2:1048684/1‑242 (MQ=255)
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              
GTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAG‑C‑A‑‑GGG‑GAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCGCT  >  NC_007779_cfb/547583‑548067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A