Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A71 F2000 I1 R1 29 128.1 2814310 92.8% 2611679 217.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_007779_cfb 547,835 +G intergenic (+264/‑1015) fdrA → / → ylbF acyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007779_cfb547,8311.G99.2% 349.6 / ‑4.0 124intergenic (+260/‑1019)fdrA/ylbFacyl‑CoA synthetase with NAD(P)‑bindingRossmann‑fold domain/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (61/62);  total (61/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 131 total aligned reads displayed.

GTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑A‑GGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCG  >  NC_007779_cfb/547583‑548065
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                           
gTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCCGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑A‑g                                                                                                                                                                                                                                            >  1:320411/1‑250 (MQ=255)
  ttACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑A‑ggg                                                                                                                                                                                                                                          >  2:582244/1‑250 (MQ=255)
   tACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑A‑gggg                                                                                                                                                                                                                                         <  1:27416/250‑1 (MQ=255)
                cAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCa                                                                                                                                                                                                                            <  2:1175853/250‑1 (MQ=255)
                 aaGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCAt                                                                                                                                                                                                                           >  1:1389107/1‑250 (MQ=255)
                   gCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCATCGAACTAAACGAGGGCACAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGCGATGACCGGACCCATGGAAGGGGCGTCCGTGGGCGCATGTCTGTTCGAAGGGTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTc                                                                                                                                                                                                                         >  1:381510/1‑250 (MQ=255)
                      aaTGCTGCGGTTCTCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCCGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCNNTNCtt                                                                                                                                                                                                                      >  2:951447/1‑250 (MQ=255)
                           tgcGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCAc                                                                                                                                                                                                                 >  2:258333/1‑250 (MQ=255)
                               gTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCAtg                                                                                                                                                                                                             <  1:724007/250‑1 (MQ=255)
                                   tCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGACGCGCAGGCGCTGGCAATACTGGACCGG‑GGGG‑GCGTGAACTTCATTCCTTGTCACCNNGNGaa                                                                                                                                                                                                         >  2:953917/1‑250 (MQ=255)
                                   tCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCNTGNCaa                                                                                                                                                                                                         >  2:1170566/1‑250 (MQ=255)
                                       aCAAATNCGTCGCGCTCGGCCACACTGGCTGGATGGGCAACCGGCTTCTCCACTTATCAGCGAACTAAACGAGCGCAGAACACTGGTTCTCGCCGGGCCGACAATGCGGTGGCAGGAGATGACGGGACCCATGAAAGGGGCGTGCGTGGGCGCGTGGCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGGTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATACCGTGTCACCATGTGagggcc                                                                                                                                                                                                     <  2:1102157/250‑6 (MQ=255)
                                          aaTTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc                                                                                                                                                                                                  <  2:215924/250‑1 (MQ=255)
                                             tcgtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCggg                                                                                                                                                                                               <  1:408786/250‑1 (MQ=255)
                                                   cgctacgccACACCGGCTAGATGTGCAACCGGCTTCGTCACGTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCGGGCGGTGGCAATACTGGTGC‑A‑GGGGTAAGTGAACTTCATTCCTTGTCACCATCTGAATGCCGTCGTGCCAATg                                                                                                                                                                                         <  2:931545/243‑1 (MQ=255)
                                                     cTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATggg                                                                                                                                                                                       >  2:1026623/1‑250 (MQ=255)
                                                             cacTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTa                                                                                                                                                                               >  2:1073401/1‑250 (MQ=255)
                                                              acTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc                                                                                                                                                                              >  1:43883/1‑250 (MQ=255)
                                                                 gggcaggtgcacAACCGGTTTCCTCCCGTAACAAGGAAATAAACGAGGGCAAAACGCTGCTGAACGCCGGGCCGCCAATGCGCTGGGAGGAGATGACCGGACCCATGAATGGGGCGAGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGGAGCGCAGGTGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCTCCATGTGAATGCCGTCGGGCCGATGGGTGGTATTAATTc                                                                                                                                                                           <  2:423889/239‑1 (MQ=255)
                                                                   ctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTg                                                                                                                                                                         <  1:1170566/250‑1 (MQ=255)
                                                                          tgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTAt                                                                                                                                                                  <  2:1389107/250‑1 (MQ=255)
                                                                                 ggCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCg                                                                                                                                                                                     <  1:676536/224‑1 (MQ=255)
                                                                                 ggCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCg                                                                                                                                                                                     >  2:676536/1‑224 (MQ=255)
                                                                                 ggCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATACCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATg                                                                                                                                                           >  1:756293/1‑250 (MQ=255)
                                                                                     tcttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCTAAGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCtgg                                                                                                                                                       >  1:944927/1‑250 (MQ=255)
                                                                                       ttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGGCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCtggtg                                                                                                                                                     <  1:953917/250‑1 (MQ=255)
                                                                                          aCTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCAtgt                                                                                                                                                                                                            <  2:1237613/192‑1 (MQ=255)
                                                                                            tttccacggAACTAAACGTGGGCAAAACACTGCTTCGCGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGGGAACTTCATTCCTTGTCGCCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTga                                                                                                                                                <  2:43883/242‑1 (MQ=255)
                                                                                                cAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAAc                                                                                                                                            >  1:713048/1‑250 (MQ=255)
                                                                                                 aGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACg                                                                                                                                           <  1:1073401/250‑1 (MQ=255)
                                                                                                        tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACtt                                                                                                                                                                                                                              <  1:223831/160‑1 (MQ=255)
                                                                                                        tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACtt                                                                                                                                                                                                                              >  2:223831/1‑160 (MQ=255)
                                                                                                           aCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACgg                                                                                                                                 >  1:1295565/1‑250 (MQ=255)
                                                                                                                 gaaaaaCCNGGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCg                                                                                                                           <  2:713048/248‑1 (MQ=255)
                                                                                                                       cacTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTa                                                                                                                     >  1:112246/1‑250 (MQ=255)
                                                                                                                          tGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGt                                                                                                                                                                    <  2:38789/200‑1 (MQ=255)
                                                                                                                          tGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGt                                                                                                                                                                    >  1:38789/1‑200 (MQ=255)
                                                                                                                            cTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCa                                                                                                                <  1:550987/250‑1 (MQ=255)
                                                                                                                              tCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGt                                                                                                                                                                                   >  2:1352805/1‑181 (MQ=255)
                                                                                                                              tCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGt                                                                                                                                                                                   <  1:1352805/181‑1 (MQ=255)
                                                                                                                              tCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAAc                                                                                                              >  2:340148/1‑250 (MQ=255)
                                                                                                                                aCGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCt                                                                                                            <  1:340148/250‑1 (MQ=255)
                                                                                                                                     gggCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACg                                                                                                       <  1:426191/250‑1 (MQ=255)
                                                                                                                                        ccgccAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAGCTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCtggtg                                                                                                                                                     <  1:26333/201‑1 (MQ=255)
                                                                                                                                        ccgccAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGCGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCtggtg                                                                                                                                                     >  2:26333/1‑201 (MQ=255)
                                                                                                                                         cgccAAGGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGACAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCTCCATGTGAATGCTGTCGGGCCAATGGGCGGTAGTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTTACCGACGGAAACCGGGCGTACTGCAACCTCAACGAAgg                                                                                                   <  2:320411/250‑1 (MQ=255)
                                                                                                                                          gccAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCaa                                                                                                         <  1:75601/243‑1 (MQ=255)
                                                                                                                                          gccAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCaa                                                                                                         >  2:75601/1‑243 (MQ=255)
                                                                                                                                                 cgcTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCa                                                                                           <  1:1026623/250‑1 (MQ=255)
                                                                                                                                                  gcTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGc                                                                                                                 >  1:1346764/1‑227 (MQ=255)
                                                                                                                                                     ggCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGt                                                                                       >  1:684920/1‑250 (MQ=255)
                                                                                                                                                        aGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGAt                                                                                    >  1:90711/1‑250 (MQ=255)
                                                                                                                                                        aGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGAt                                                                                    >  1:1129502/1‑250 (MQ=255)
                                                                                                                                                           agaTGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCg                                                                                 >  2:20424/1‑250 (MQ=255)
                                                                                                                                                                aCCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGACTGTTCGAAGGTTGGGCGAAAGATGAAGAGCAAGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGGATTCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGGACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTg                                                                            >  2:347029/1‑250 (MQ=255)
                                                                                                                                                                  cGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCTATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGAt                                                                                    <  1:98005/240‑1 (MQ=255)
                                                                                                                                                                  cGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCTATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGAt                                                                                    >  2:98005/1‑240 (MQ=255)
                                                                                                                                                                  cGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGAc                                                                                                                                       >  2:492610/1‑189 (MQ=255)
                                                                                                                                                                  cGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGAc                                                                                                                                       <  1:492610/189‑1 (MQ=255)
                                                                                                                                                                  cGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGaa                                                                                                     <  1:632207/223‑1 (MQ=255)
                                                                                                                                                                    gACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGAt                                                                                                                                                            >  1:1027925/1‑166 (MQ=255)
                                                                                                                                                                       ccATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTa                                                                     >  2:113950/1‑250 (MQ=255)
                                                                                                                                                                            aaaGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGc                                                                                                                                                                        <  1:1201275/146‑1 (MQ=255)
                                                                                                                                                                                 ggcgggcgTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCaa                                                                                          <  2:488610/219‑1 (MQ=255)
                                                                                                                                                                                 ggCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCaa                                                                                          >  1:488610/1‑219 (MQ=255)
                                                                                                                                                                                          gggCGCATGTCTGTTCGAAGGTTGGGCGAAATATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGAcc                                                                                                                                      >  1:936398/1‑166 (MQ=255)
                                                                                                                                                                                          gggCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTGGAGAACGTGAcc                                                                                                                                      <  2:936398/166‑1 (MQ=255)
                                                                                                                                                                                               cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGGGAACGTGACCGACGGCaa                                                                                                                              >  1:1249787/1‑169 (MQ=255)
                                                                                                                                                                                               cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCGGCCAGTATGCCGATGCTGGTGGTTGGGAACGGGACCGACGGCaa                                                                                                                              <  2:1249787/169‑1 (MQ=255)
                                                                                                                                                                                                aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTAc                                                                                                                    >  1:448639/1‑178 (MQ=255)
                                                                                                                                                                                                aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTAc                                                                                                                    <  2:448639/178‑1 (MQ=255)
                                                                                                                                                                                                     tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTg                                       <  2:684920/250‑1 (MQ=255)
                                                                                                                                                                                                        tCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGAt                                    <  1:20424/250‑1 (MQ=255)
                                                                                                                                                                                                         cGAAGGTTGGGCGAAAGATGAAGCGCTGGGGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTGTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATg                                   <  2:1260004/250‑1 (MQ=255)
                                                                                                                                                                                                           aaGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTa                                                                                                 >  1:57722/1‑186 (MQ=255)
                                                                                                                                                                                                           aaGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTa                                                                                                 <  2:57722/186‑1 (MQ=255)
                                                                                                                                                                                                              gTTGGGCGAAGGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGa                              >  1:1213182/1‑250 (MQ=255)
                                                                                                                                                                                                                tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTg                                                                                                                                                 <  1:935769/133‑1 (MQ=255)
                                                                                                                                                                                                                tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTg                                                                                                                                                 >  2:935769/1‑133 (MQ=255)
                                                                                                                                                                                                                tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGAtg                            <  2:751083/250‑1 (MQ=255)
                                                                                                                                                                                                                 gggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACg                                                                   <  1:1129060/210‑1 (MQ=255)
                                                                                                                                                                                                                 gggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACg                                                                   >  2:1129060/1‑210 (MQ=255)
                                                                                                                                                                                                                       aaGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATc                                                                                               <  1:595678/176‑1 (MQ=255)
                                                                                                                                                                                                                       aaGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATc                                                                                               >  2:595678/1‑176 (MQ=255)
                                                                                                                                                                                                                       aaGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGATGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGACGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGCTGTCCTGACTCGCCATCGCTGGGGGCGCGGTGTGttcag                     >  2:1285163/1‑247 (MQ=255)
                                                                                                                                                                                                                         gATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTaa                      >  1:1063481/1‑247 (MQ=255)
                                                                                                                                                                                                                         gATGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTaa                      <  2:1063481/247‑1 (MQ=255)
                                                                                                                                                                                                                           tGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGa                                     >  1:334072/1‑230 (MQ=255)
                                                                                                                                                                                                                           tGAAGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGa                                     <  2:334072/230‑1 (MQ=255)
                                                                                                                                                                                                                             aaGCGCAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGt               <  2:1295565/250‑1 (MQ=255)
                                                                                                                                                                                                                                  cAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACt                                                  <  1:1356861/210‑1 (MQ=255)
                                                                                                                                                                                                                                  cAGGCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACt                                                  >  2:1356861/1‑210 (MQ=255)
                                                                                                                                                                                                                                   agacgcCGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTAGAAGAAAAGAGGAGAAAGCCGTCGGGCCAATGGGCGGTTTTACTTGTGCCAGTATGCTGATGAAGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGAATCGGCAAAGTGCTGCGTTTTGGCGCTTACGGCGAAGATGTCCTGTATCCCCATCGCTGCATTCGCGATGTGTTAATGCCAGAATTAAg         >  2:328366/4‑250 (MQ=255)
                                                                                                                                                                                                                                    ggCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCg                                                                                 >  1:756936/1‑177 (MQ=255)
                                                                                                                                                                                                                                    ggCGCTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCg                                                                                 <  2:756936/177‑1 (MQ=255)
                                                                                                                                                                                                                                      cgcTGGCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAgcgc      >  1:1402540/1‑250 (MQ=255)
                                                                                                                                                                                                                                          agCAATACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCgcggcg  <  2:1213182/249‑1 (MQ=255)
                                                                                                                                                                                                                                              aTACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTAc                                                                                                                    >  1:562497/1‑132 (MQ=255)
                                                                                                                                                                                                                                              aTACTGGAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTAc                                                                                                                    <  2:562497/132‑1 (MQ=255)
                                                                                                                                                                                                                                                   ggAGC‑AGGGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTg                                                                                      <  1:257985/157‑1 (MQ=255)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                           
GTTTACATCAGTGGCGCAAGCCAATGCTGCGGTTATCGAACAAATTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGGGGCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGC‑A‑GGGG‑AAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTCTGCCAGTATGCCGATGCTGGTGGTTGAGAACGTGACCGACGGCAACCGGGCGTACTGCAACCTCAACGAAGGTATCGGCAAAGTGATGCGTTTTGGCGCTTACGGCGAAGATGTCCTGACTCGCCATCGCTGGATGCGCGATGTGTTAATGCCAGTATTAAGCGCGGCG  >  NC_007779_cfb/547583‑548065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A