Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A69 F2000 I2 R1	32	130.0	2789900	93.6%	2611346	220.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_007779_cfb	2,468,335	(C)_9→10	intergenic (+243/‑123)	fadL → / → yfdF	long‑chain fatty acid outer membranetransporter/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_007779_cfb	2,468,326	1	.	C	83.3%	108.4 / 20.3	48	intergenic (+234/‑132)	fadL/yfdF	long‑chain fatty acid outer membranetransporter/hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (2/6); new base C (19/21); total (21/27)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTC  >  NC_007779_cfb/2468078‑2468556

aaCTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑c                                                                                                                                                                                                                                        >  2:1340544/1‑250 (MQ=255)

 aCTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAACAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cc                                                                                                                                                                                                                                       <  1:952737/250‑1 (MQ=255)

   tACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cccc                                                                                                                                                                                                                                     >  1:831229/1‑250 (MQ=255)

          tCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGCTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cccccc‑cccct                                                                                                                                                                                                                             >  1:1093352/1‑248 (MQ=255)

            tGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑CCCCCC‑CCCTGGc                                                                                                                                                                                                                           <  2:988190/250‑1 (MQ=255)

             gATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑CCCCCC‑CCCTGGCt                                                                                                                                                                                                                          >  2:330552/1‑250 (MQ=255)

                             ggATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑CCCCCC‑CCCTGGCTATTGTGCGCTCATaca                                                                                                                                                                                                          <  1:1340544/250‑1 (MQ=255)

                                       aCCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTaa                                                                                                                                                                                                <  1:330552/250‑1 (MQ=255)

                                                    gtcaCTNTAACCCCCCCACTTACCCGAAAGTGTTGTCCTCATTGCGTGCCTCCGGGATTTTACAAACAGACGGTTGGCAAGTTCCCCTTTCAAATCAAAAAGCGGTAAGCACGAATAATCCAGAAGGGAAAAGCATATTTTCGGGCGTTCTGCTCGGCCTTCCCAAAAACTTTACTTTTATGAATTTCCAGGAAACT‑‑CCCCCCACCCTGGATATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCta                                                                                                                                                                                    <  2:1093352/247‑1 (MQ=255)

                                                         tttAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaataa                                                                                                                                                                               <  1:1267686/250‑1 (MQ=255)

                                                            aaCTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATg                                                                                                                                                                            >  2:303437/1‑250 (MQ=255)

                                                                cccccACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGt                                                                                                                                                                       <  2:95869/250‑1 (MQ=255)

                                                                             gAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGACGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGTTAGGTTCTAAATAAATGAGCGTTTTTTGATAGTc                                                                                                                                                           >  1:199046/1‑250 (MQ=255)

                                                                                         cTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                                                                                                                               <  1:753474/250‑1 (MQ=255)

                                                                                             ttGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAat                                                                                                                                           <  1:801930/250‑1 (MQ=255)

                                                                                                  tACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatatata                                                                                                                                      <  2:552656/250‑1 (MQ=255)

                                                                                                   aCCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTTTTGTGCGCTCATCTACTCAACTTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatatatat                                                                                                                                     >  1:799152/1‑250 (MQ=255)

                                                                                                    cctcctGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGATCGTTTTTTGATAGTCTATTTCATTAGGTAATATATAtt                                                                                                                                    >  2:209009/1‑250 (MQ=255)

                                                                                                           aGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑c                                                                                                                                                                                                                                        <  2:993606/143‑1 (MQ=255)

                                                                                                           aGTTTTGCAAACAGACTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑c                                                                                                                                                                                                                                        >  1:993606/1‑143 (MQ=255)

                                                                                                             ttttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTTCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACaaa                                                                                                                           >  1:497960/1‑250 (MQ=255)

                                                                                                              tttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCC‑CCCTGGTTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAACTGAGCGTTTTTTGATAGTCTATTTCATTAGTTAATAAATATTGTTATCaaa                                                                                                                           >  2:728049/1‑250 (MQ=255)

                                                                                                               nttcANACAGCCTGTTGGCAGCTTACCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatc                                                                                                                         <  2:799152/247‑1 (MQ=255)

                                                                                                                              tGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATa                                                                                                          <  1:303437/250‑1 (MQ=255)

                                                                                                                                     ttGCCCTTTCAAATCGATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAg                                                                                                                                                                                              >  1:682237/1‑159 (MQ=255)

                                                                                                                                     ttGCCCTTTCAAATCGATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAg                                                                                                                                                                                              <  2:682237/159‑1 (MQ=255)

                                                                                                                                        cccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCt                                                                                                <  1:209009/250‑1 (MQ=255)

                                                                                                                                             tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAAt                                                                                                           >  2:844516/1‑234 (MQ=255)

                                                                                                                                             tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAAt                                                                                                           <  1:844516/234‑1 (MQ=255)

                                                                                                                                                 aTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATac                                                                                                                                                                                                           <  1:1182018/134‑1 (MQ=255)

                                                                                                                                                 aTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATac                                                                                                                                                                                                           >  2:1182018/1‑134 (MQ=255)

                                                                                                                                                     aTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                                                                                                                    >  1:335345/1‑184 (MQ=255)

                                                                                                                                                     aTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                                                                                                                    <  2:335345/184‑1 (MQ=255)

                                                                                                                                                        aGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGCGCGCTCATACACTCAAATTAAAGTTAGGTTCTACATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGTAAATATATATTTGTACCAAATCAATCAAAATGGAAAAAAATAATGCTACCATCTATTccaatc                                                                                >  2:1184798/1‑250 (MQ=255)

                                                                                                                                                                ccACAGACACACCTGAAGGGACAGGCTTATTTTCAAGCGTTCTCATCTTCATTCTCAAATAGTTTAATTTTCTGCATTTCCAGGATATTC‑CCCCCC‑CCCTGTCTCTAGTGCGTTCATTCACTAAAATTACAGATAGGTTCTAAATAAATGAGCGTTTTTTAATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATTCTACCATCTATTTCAATaannanaaa                                                                        >  2:932720/1‑246 (MQ=255)

                                                                                                                                                                ccACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTc                                                                                                                                                                                                       >  2:985080/1‑123 (MQ=255)

                                                                                                                                                                ccACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTc                                                                                                                                                                                                       <  1:985080/123‑1 (MQ=255)

                                                                                                                                                                      aCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                                                                                                                       <  1:396696/133‑1 (MQ=255)

                                                                                                                                                                      aCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                                                                                                                       >  2:396696/1‑133 (MQ=255)

                                                                                                                                                                                       ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCa                                                 <  1:1184798/250‑1 (MQ=255)

                                                                                                                                                                                          atatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatat                                                                                                                                         <  1:496380/159‑1 (MQ=255)

                                                                                                                                                                                          atatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGCTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatat                                                                                                                                         >  2:496380/1‑159 (MQ=255)

                                                                                                                                                                                              tttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatata                                                                                                                                        >  2:1212404/1‑156 (MQ=255)

                                                                                                                                                                                              tttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatata                                                                                                                                        <  1:1212404/156‑1 (MQ=255)

                                                                                                                                                                                              tttCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATg                                           <  1:728049/250‑1 (MQ=255)

                                                                                                                                                                                                 cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGaaaa                                       >  2:425256/1‑250 (MQ=255)

                                                                                                                                                                                                    gCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGTTAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCAt                                                >  2:549035/1‑238 (MQ=255)

                                                                                                                                                                                                    gCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCAt                                                <  1:549035/238‑1 (MQ=255)

                                                                                                                                                                                                      nttCTNCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACa                                  <  2:808378/249‑1 (MQ=255)

                                                                                                                                                                                                                    tctcAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAAt                    <  1:425256/250‑1 (MQ=255)

                                                                                                                                                                                                                          agagTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaat                                                                                                                      >  1:591717/1‑146 (MQ=255)

                                                                                                                                                                                                                          agagTTTACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaat                                                                                                                      <  2:591717/146‑1 (MQ=255)

                                                                                                                                                                                                                               ttACTTTTCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACa         >  2:770033/1‑250 (MQ=255)

                                                                                                                                                                                                                                     ttCTGCATTTCCAGGATACTC‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATG‑AAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTc  <  2:831229/250‑1 (MQ=255)

AACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑CCCCCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCATCTATTTCAATCAACAATACCAGCGCAGCTTACCCAGAATCCATCAATGAAAATAACAATGATGAAGTTAATGGATTAGTACAAGAGTTC  >  NC_007779_cfb/2468078‑2468556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A