Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F999 I1 R1
|
225 |
156.4 |
11709729 |
83.8% |
9812752 |
75.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,352,254 |
2 bp→GC |
intergenic (‑569/‑12) |
yhcC ← / → gltB |
hypothetical protein/glutamate synthase large subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,352,254 | 0 | C | G | 99.1%
| 311.7
/ ‑4.6
| 114 | intergenic (‑569/‑13) | yhcC/gltB | hypothetical protein/glutamate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base G (61/52); total (61/53) |
| Fisher's exact test for biased strand distribution p-value = 4.65e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 |
| * | NC_000913 | 3,352,255 | 0 | G | C | 99.1%
| 314.6
/ ‑4.7
| 114 | intergenic (‑570/‑12) | yhcC/gltB | hypothetical protein/glutamate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base C (61/52); total (61/53) |
| Fisher's exact test for biased strand distribution p-value = 4.65e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 |
Only 100 of 114 total aligned reads displayed.
TATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATCG‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGA > NC_000913/3352187‑3352322
||
tATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGgcgc < 2:317400/76‑1 (MQ=255)
tATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGgcgc < 2:2212787/76‑1 (MQ=255)
aTGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGgcgcg > 1:3803351/1‑76 (MQ=255)
tGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGgcgcgc < 2:2249081/76‑1 (MQ=255)
gCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGcgcgcg > 2:3014103/1‑76 (MQ=255)
gCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGcgcgcg > 1:2906671/1‑76 (MQ=255)
cAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGt > 1:1422045/1‑76 (MQ=255)
cAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGt < 2:3835603/76‑1 (MQ=255)
aGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTa > 1:3513738/1‑76 (MQ=255)
aGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTa < 1:2763509/76‑1 (MQ=255)
tAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATg > 2:4841086/1‑76 (MQ=255)
aaTTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGa < 1:2590107/76‑1 (MQ=255)
aaTTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGa > 1:3738060/1‑76 (MQ=255)
tGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGacac > 2:3962960/1‑76 (MQ=255)
tGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGacac < 1:251128/76‑1 (MQ=255)
gagaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACg > 1:5401408/1‑76 (MQ=255)
gagaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACg > 2:581609/1‑76 (MQ=255)
agaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAA‑CGCGAGGCGCGCGTATGACACGc < 1:663482/76‑1 (MQ=255)
gaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCa < 1:1825330/76‑1 (MQ=255)
aTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCaa > 2:4282606/1‑76 (MQ=255)
aTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCaa < 2:5520284/76‑1 (MQ=255)
aTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCaa < 2:5117128/76‑1 (MQ=255)
ccccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAAc > 2:1586486/1‑76 (MQ=255)
ccccGCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAAc < 2:5569742/76‑1 (MQ=255)
cccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCaaa < 2:4865508/74‑1 (MQ=255)
cccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCaaa > 1:4865509/1‑74 (MQ=255)
cccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAAcc > 2:2446553/1‑76 (MQ=255)
cccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAAcc > 1:4751276/1‑76 (MQ=255)
cccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAAcc < 2:3906524/76‑1 (MQ=255)
ccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAAccc < 1:1928750/76‑1 (MQ=255)
ccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAAccc > 1:4804521/1‑76 (MQ=255)
ccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAAccc > 2:234101/1‑76 (MQ=255)
ctctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAAcccc < 1:5276660/76‑1 (MQ=255)
ctctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAAcccc < 1:5227285/76‑1 (MQ=255)
ctctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAAcccc < 1:3085563/76‑1 (MQ=255)
tctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCg < 1:5389236/76‑1 (MQ=255)
tctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCg > 1:2651096/1‑76 (MQ=255)
ctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGt < 2:5607694/76‑1 (MQ=255)
ttCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTc > 1:2287202/1‑76 (MQ=255)
tCACCGTATTAACCGATGCGAAAAGGACAACAATGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCg < 1:3472537/76‑1 (MQ=255)
tCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCg < 1:4110992/76‑1 (MQ=255)
aCCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGcc > 1:5019248/1‑76 (MQ=255)
aCCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGcc < 1:3028219/76‑1 (MQ=255)
ccGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCa > 1:1656166/1‑76 (MQ=255)
cGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCAc < 2:2282634/76‑1 (MQ=255)
cGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCAc < 2:4961307/76‑1 (MQ=255)
cGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGCATGACACGCAAACCCCGTCGCCAc > 2:2902670/1‑76 (MQ=255)
tATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGc > 2:2812522/1‑76 (MQ=255)
aTTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGct > 1:4657930/1‑76 (MQ=255)
ttAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGctc > 1:5186605/1‑76 (MQ=255)
ttAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGctc < 1:5357621/76‑1 (MQ=255)
ttAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGctc < 2:2300134/76‑1 (MQ=255)
ttAACCGAGGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAGCCCCGTCGCCACGctc < 2:4290389/76‑1 (MQ=255)
tAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGctct > 2:2886845/1‑76 (MQ=255)
aaCCGATGCGAAAATGACAACAATGGGGCGCATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCtt < 1:3736036/76‑1 (MQ=255)
aaCCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCtt > 1:3450335/1‑76 (MQ=255)
aaCCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCtt > 1:3099323/1‑76 (MQ=255)
aCCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCttt > 1:28775/1‑76 (MQ=255)
aCCGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCCCttt > 2:520555/1‑76 (MQ=255)
ccGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGTCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTc < 1:3434481/76‑1 (MQ=255)
ccGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTc > 1:1659780/1‑76 (MQ=255)
ccGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTc < 1:2979183/76‑1 (MQ=255)
ccGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCCTCGCCACGCTCTTTc < 1:1966517/76‑1 (MQ=255)
cGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt > 1:3709108/1‑76 (MQ=255)
cGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt > 2:1431732/1‑76 (MQ=255)
cGATGCGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt < 2:2906671/76‑1 (MQ=255)
cGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGcc > 2:4738140/1‑76 (MQ=255)
cGAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGcc > 2:4476107/1‑76 (MQ=255)
gAAAAGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGccc < 2:3919780/76‑1 (MQ=255)
aaaaGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCg < 2:4500764/76‑1 (MQ=255)
aaaaGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCg < 2:2699713/76‑1 (MQ=255)
aaaGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGt < 1:789650/76‑1 (MQ=255)
aaaGGACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGt < 1:2681686/76‑1 (MQ=255)
ggACAACGAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcg < 2:1180044/76‑1 (MQ=255)
ggACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcg > 1:79559/1‑76 (MQ=255)
gACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAACCCCCGTCCCCACGCTCTTTCTGTGCCCgcgcgc > 2:5590871/1‑76 (MQ=255)
gACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcgc < 1:4023031/76‑1 (MQ=255)
gACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcgc < 2:1695258/76‑1 (MQ=255)
gACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcgc > 1:3095156/1‑76 (MQ=255)
gACAACAAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcgc < 1:1452745/76‑1 (MQ=255)
acaacaAGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcgcc > 2:2250940/1‑75 (MQ=255)
caacaaGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAg < 1:3361050/76‑1 (MQ=255)
aacaaGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAGCCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGc < 2:1193107/76‑1 (MQ=255)
aacaaGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGc < 1:3397604/76‑1 (MQ=255)
acaaGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCg < 1:234101/76‑1 (MQ=255)
acaaGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCg > 1:5131685/1‑76 (MQ=255)
caaGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCgg > 1:2193772/1‑76 (MQ=255)
caaGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCgg > 2:4388944/1‑76 (MQ=255)
aaGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGt > 1:5771171/1‑76 (MQ=255)
aaGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGt < 1:4148026/76‑1 (MQ=255)
aGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCgggt > 1:983883/1‑74 (MQ=255)
aGGGGGCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGCGCCCGTGCGCAGCGGtt > 1:2113539/1‑76 (MQ=255)
gggggCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTc > 1:79946/1‑76 (MQ=255)
gggggCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTc > 1:1691424/1‑76 (MQ=255)
gggggCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTc > 1:25658/1‑76 (MQ=255)
ggggCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCg < 2:3738060/76‑1 (MQ=255)
ggggCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCg < 1:2897942/76‑1 (MQ=255)
gggCGAATGC‑GAGGCGCGCGTATGACGGGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCgg < 2:1035786/76‑1 (MQ=255)
gggCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCgg > 1:5018200/1‑76 (MQ=255)
ggCGAATGC‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGa > 1:5588001/1‑76 (MQ=255)
||
TATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATCG‑GAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGA > NC_000913/3352187‑3352322
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
