Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F999 I1 R1
|
226 |
132.7 |
9745491 |
85.7% |
8351885 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,352,254 |
2 bp→GC |
intergenic (‑569/‑12) |
yhcC ← / → gltB |
hypothetical protein/glutamate synthase large subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,352,254 | 0 | C | G | 99.1%
| 296.9
/ NA
| 113 | intergenic (‑569/‑13) | yhcC/gltB | hypothetical protein/glutamate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (63/49); minor base A (1/0); total (64/49) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| * | NC_000913 | 3,352,255 | 0 | G | C | 99.1%
| 292.9
/ NA
| 112 | intergenic (‑570/‑12) | yhcC/gltB | hypothetical protein/glutamate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (63/48); minor base T (1/0); total (64/48) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
Only 100 of 113 total aligned reads displayed.
TTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATCGGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGA > NC_000913/3352185‑3352322
||
ttATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcg < 1:766137/76‑1 (MQ=255)
tATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgc < 2:74822/76‑1 (MQ=255)
tATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgc < 2:2587052/76‑1 (MQ=255)
aTGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcg > 1:747639/1‑76 (MQ=255)
tGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcgc > 1:2840207/1‑76 (MQ=255)
tGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcgc < 1:7061/76‑1 (MQ=255)
tGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcgc < 2:3727748/76‑1 (MQ=255)
tGCAGTAATTGAGACCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcgc < 1:99465/76‑1 (MQ=255)
cAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGt > 1:1548771/1‑76 (MQ=255)
gTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTAt > 1:2966983/1‑76 (MQ=255)
gTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTAt > 1:3297793/1‑76 (MQ=255)
gTAAGTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCCAGGCGCGCGTAt > 2:421982/1‑76 (MQ=255)
tAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcgc > 2:153482/1‑71 (MQ=255)
tAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGgcgcgc < 1:153482/71‑1 (MQ=255)
tAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg < 1:2210890/76‑1 (MQ=255)
tAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg < 1:1734352/76‑1 (MQ=255)
tAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg > 1:2494054/1‑76 (MQ=255)
tAATTGAGATCCCCGCTTCACCGTATGAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATg < 1:1744197/76‑1 (MQ=255)
aTTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGac > 2:2713498/1‑76 (MQ=255)
ttGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGaca < 2:4401234/76‑1 (MQ=255)
gagaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACg < 1:1878751/76‑1 (MQ=255)
gagaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACg < 2:1184297/76‑1 (MQ=255)
agaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGc < 2:4457493/76‑1 (MQ=255)
agaTCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGc < 1:2714651/76‑1 (MQ=255)
gaTCCCCTCTTCACCGTATTAACCGATGCGAAAACGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCa > 1:3003802/1‑76 (MQ=255)
gaTCCCATCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCa > 1:4052545/1‑76 (MQ=255)
tCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCaaa > 1:1480875/1‑76 (MQ=255)
tCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCaaa < 2:3024632/76‑1 (MQ=255)
ccccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAc < 2:4786448/76‑1 (MQ=255)
cccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAcc > 1:1882887/1‑76 (MQ=255)
ccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAccc > 2:1998361/1‑76 (MQ=255)
ccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAccc > 1:1687113/1‑76 (MQ=255)
ccTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAccc < 1:3749367/76‑1 (MQ=255)
ctctTGACCGTGTGGACCGATGCGAAAAGGACAACAAGGGGGCGAATGGGAGGCGCGAGTATGACACGCAAAcccc < 2:2269311/76‑1 (MQ=255)
ctctTCACCGTATTAGCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAcccc < 2:3971576/76‑1 (MQ=255)
tctTCACCGTATTAACCGATGCGAAAAGGACACCCAGGGGGCGAATGCGAGGCGCGCGTATGACCGGCAACCCCCg > 2:243239/1‑76 (MQ=255)
tctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCg > 1:1248446/1‑76 (MQ=255)
tctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCg > 1:2205115/1‑76 (MQ=255)
tctTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATACGAGGCGCGCGTATGACACGCAAACCCCg > 1:128421/1‑76 (MQ=255)
ctTCACCGTAGTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGt < 2:4291953/76‑1 (MQ=255)
ttCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTc < 1:350733/76‑1 (MQ=255)
tCACCGTATTAACCGATGCGAACAGGACAACAAGGGGGACCATGCGAGGCGCGCGTCTGACACGCAACCCCCgcac > 1:374460/1‑73 (MQ=255)
tCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAcccc > 1:4800395/1‑72 (MQ=255)
tCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAAcccc < 2:4800395/72‑1 (MQ=255)
cACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAAGCGAGGCGCGCGTATGACACGCAAACCCcgccgc > 2:4702400/1‑76 (MQ=255)
aCCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAAGGCGAGGCGCGCGTCTGACACGCAAACCCCGTCGcc > 2:4229725/1‑76 (MQ=255)
ccGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCa > 1:4129523/1‑76 (MQ=255)
tgTATTAACCGATGCGAAAAGGACAACATGGAGGCCTATGTGAAGAGCGAGTATGGCTCGCACACCACGTTGCCAc > 1:618657/2‑76 (MQ=255)
cGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCCAACCCCGTCGCCAc > 2:1538385/1‑76 (MQ=255)
cGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCAc < 2:4467751/76‑1 (MQ=255)
cGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCAc < 1:4686308/76‑1 (MQ=255)
cGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCAc > 1:2266570/1‑76 (MQ=255)
gTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCAcc > 2:3270444/1‑75 (MQ=255)
gTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACg < 1:2551507/76‑1 (MQ=255)
tAACCGATGCGAGAAGGACAACAAGGGGGCGAATGCGAGGGGCGGGTATGACACGCAAACCGCGTCGCCACGctct < 2:3365783/76‑1 (MQ=255)
aaCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCCAACCCCGTCGCCACGCTCtt < 2:3003802/76‑1 (MQ=255)
aaCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCtt > 1:1298752/1‑76 (MQ=255)
aaCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGCCCCCACGCTCtt > 2:4506631/1‑76 (MQ=255)
aCCGATGCGAAAAGGACAACAAGTGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCttt < 2:4129523/76‑1 (MQ=255)
aCCGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCttt > 2:3433510/1‑76 (MQ=255)
cGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt > 2:743958/1‑76 (MQ=255)
cGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt > 1:4668512/1‑76 (MQ=255)
cGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt > 2:3283920/1‑76 (MQ=255)
cGATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCt < 1:3805660/76‑1 (MQ=255)
gttGCGAAAAGGCCAGCAAGGGGGCGAAGGCGCGGCGGGCGTATGTCACGCCCACCCCGTCGCCCCGCTCTTtgtg < 2:3872598/74‑1 (MQ=255)
gATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACCCGCAAACCCCGCCCCCCCGCCCTTTCtg > 2:174427/1‑76 (MQ=255)
gATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtg > 1:3456545/1‑76 (MQ=255)
gATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCCTTCtg > 1:3255331/1‑76 (MQ=255)
gATGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTAGCCACGCTCTTTCtc > 1:3164050/1‑75 (MQ=255)
aTGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgt < 1:940400/76‑1 (MQ=255)
aTGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgt < 2:1935613/76‑1 (MQ=255)
aTGCGAAAAGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTCTCtgt > 2:2572257/1‑76 (MQ=255)
aTGCGAAAAAGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCtgt < 2:4756455/76‑1 (MQ=255)
aaaaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCg < 1:3585955/76‑1 (MQ=255)
aaaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGt > 2:2170716/1‑76 (MQ=255)
aaaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGt < 1:2150785/76‑1 (MQ=255)
aaGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTg > 2:2284975/1‑76 (MQ=255)
aaGGACAACAAGGGGGCGAAGGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGACCgcg > 2:286487/1‑74 (MQ=255)
aGGACAACACGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgc > 1:415247/1‑76 (MQ=255)
aGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgc < 2:1176706/76‑1 (MQ=255)
aGGACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgc > 1:3708601/1‑76 (MQ=255)
gACAACAAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcgc > 1:2136825/1‑76 (MQ=255)
acaacaAGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCgcgcgcc > 1:24456/1‑75 (MQ=255)
caacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGCCGCCACGCTCTTTCTGCGCCCGTGCGCAg > 2:3865583/1‑76 (MQ=255)
aacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTgcgc < 2:165213/73‑1 (MQ=255)
aacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAga < 2:3029478/76‑2 (MQ=255)
aacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGc > 1:599424/1‑76 (MQ=255)
aacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGc > 1:1137070/1‑76 (MQ=255)
aacaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGc > 2:678567/1‑76 (MQ=255)
acaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCg > 1:3753082/1‑76 (MQ=255)
acaaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCg > 1:3219566/1‑76 (MQ=255)
caaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCgg > 1:4123962/1‑76 (MQ=255)
caaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCgg < 1:1733000/76‑1 (MQ=255)
caaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCgg > 1:3536699/1‑76 (MQ=255)
aaGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGt < 2:4259599/76‑1 (MQ=255)
aGGGGGCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGtt < 1:2353218/76‑1 (MQ=255)
gggggCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTc > 2:3167762/1‑76 (MQ=255)
ggCGAATGCGAGGCGCGCGTATGGCACGCAAGCCCCGGCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGa < 2:3419186/76‑1 (MQ=255)
ggCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGa < 1:1297241/76‑1 (MQ=255)
ggCGAATGCGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGa > 2:2116408/1‑76 (MQ=255)
||
TTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATCGGAGGCGCGCGTATGACACGCAAACCCCGTCGCCACGCTCTTTCTGTGCCCGTGCGCAGCGGTTCGGA > NC_000913/3352185‑3352322
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
