Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F999 I1 R1 225 156.4 11709729 83.8% 9812752 75.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,537,405 +GC intergenic (+61/‑394) b3407 → / → feoA hypothetical protein/ferrous iron transport protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,537,4041.C97.9% 235.6 / ‑0.6 95intergenic (+60/‑395)b3407/feoAhypothetical protein/ferrous iron transport protein A
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base C (41/52);  total (41/54)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
*NC_0009133,537,4042.G97.9% 230.8 / ‑0.5 95intergenic (+60/‑395)b3407/feoAhypothetical protein/ferrous iron transport protein A
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (41/52);  total (41/54)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 106 total aligned reads displayed.

GGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑C‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATT  >  NC_000913/3537330‑3537470
                                                                           ||                                                                     
ggCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑c                                                                      <  2:1819823/76‑1 (MQ=255)
ggCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGCTCA‑‑c                                                                      >  1:2862488/1‑76 (MQ=255)
 gCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑cg                                                                     <  1:3712299/76‑2 (MQ=255)
  cAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑cga                                                                    <  2:1582937/76‑3 (MQ=255)
  cAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑cg                                                                     >  2:3013543/1‑74 (MQ=255)
   aaaaagCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑cgcc                                                                   <  2:1696064/76‑4 (MQ=255)
   aaaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑cgcc                                                                   <  2:5445623/76‑4 (MQ=255)
   aaaaaaCGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGCCGTT‑A‑‑C‑‑C‑Gcc                                                               >  2:22642/1‑75 (MQ=255)
   aaaaaaCGTGAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑cgcc                                                                   <  2:4998400/76‑4 (MQ=255)
            tAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGc                                                         >  2:1084222/1‑76 (MQ=37)
            tAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGc                                                         >  2:1817089/1‑76 (MQ=37)
            tAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGc                                                         <  1:4603806/76‑1 (MQ=37)
             aaaCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCa                                                        <  1:3748539/76‑1 (MQ=25)
              aaCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCaa                                                       >  2:63211/1‑76 (MQ=25)
              aaCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCaa                                                       <  1:41765/76‑1 (MQ=25)
               aCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCaac                                                      <  2:3867459/76‑1 (MQ=25)
               aCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCaac                                                      >  1:5812445/1‑76 (MQ=25)
               aCGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCaac                                                      >  2:5687236/1‑76 (MQ=25)
               aCGCCCGTACGGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCaac                                                      <  2:2722305/76‑1 (MQ=25)
                cGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCaacg                                                     >  1:2185491/1‑76 (MQ=25)
                 gCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCaacgg                                                    <  1:5153445/76‑2 (MQ=25)
                 gCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCaacga                                                    >  2:2344777/1‑76 (MQ=25)
                 gCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCaacga                                                    >  1:1261701/1‑76 (MQ=25)
                 ccgcGTAGTGCCCTCCAGTTGGAATTTGCACACAATGGTAGGACAGATCAGGCGTTCA‑‑CGGC‑GCATCTGGCaacga                                                    <  1:1109970/73‑1 (MQ=9)
                  cccGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCaacgaa                                                   >  1:4283670/1‑76 (MQ=25)
                  cccGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCaacgaa                                                   >  1:5442498/1‑76 (MQ=25)
                  cccGTACTGGCCTACGATTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCaacgaa                                                   <  1:4966709/76‑1 (MQ=25)
                   ccGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCaacgaac                                                  <  1:2744133/76‑1 (MQ=25)
                   ccGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCaacgaac                                                  <  2:611737/76‑1 (MQ=25)
                   ccGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCaacgaac                                                  <  1:1436984/76‑1 (MQ=25)
                   ccGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCaacgaac                                                  <  1:4245382/76‑1 (MQ=25)
                    cacaCTGCCCTACGGATCGAATTTGAACGAAATCGTAGGGGCGATAAGGGTTTCACGC‑‑C‑GCATCTGGCaacgaacg                                                 <  1:1060108/73‑1 (MQ=16)
                    cGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGACAAGGCGTTCACGC‑‑C‑GCATCTGGCaacgaacg                                                 >  2:1652704/1‑76 (MQ=25)
                     gTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGc                                                <  1:829444/76‑1 (MQ=25)
                      tACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGcc                                               >  1:4850314/1‑76 (MQ=25)
                       aCTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCt                                              <  1:5019918/76‑1 (MQ=25)
                           gCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGcc                                          >  1:1918847/1‑76 (MQ=25)
                           gCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGcc                                          <  1:5413266/76‑1 (MQ=25)
                           gCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGcc                                          <  1:2684134/76‑1 (MQ=25)
                            gcgaCGGTTCGAATTGTCAGTGAATCGTAGGGCAGATAGGGCGTTCACGC‑‑C‑GCGTCTGGCAACGAACGCCTTGCCt                                         <  2:3140873/73‑1 (MQ=12)
                            ccgaCGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCt                                         <  2:421220/73‑1 (MQ=25)
                             cTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTg                                        >  1:1653662/1‑76 (MQ=25)
                             cTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTg                                        >  1:5554415/1‑76 (MQ=25)
                             cTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTg                                        <  1:4402908/76‑1 (MQ=25)
                              tACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGa                                       <  1:4733967/76‑1 (MQ=25)
                              tACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGa                                       <  2:4912786/76‑1 (MQ=25)
                              tACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGGTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTgc                                       >  2:1338656/1‑75 (MQ=25)
                                cGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACa                                     >  2:1387750/1‑76 (MQ=25)
                                 ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACAt                                    <  2:3870774/76‑1 (MQ=25)
                                 ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACAt                                    >  2:1619185/1‑76 (MQ=25)
                                 ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACAt                                    <  1:5013749/76‑1 (MQ=25)
                                 ggTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACAt                                    >  1:5106343/1‑76 (MQ=25)
                                  gTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATa                                   <  1:3865696/76‑1 (MQ=25)
                                  gTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATa                                   >  2:3595155/1‑76 (MQ=25)
                                   ttCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATaa                                  >  1:1099904/1‑76 (MQ=25)
                                   ttCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATaa                                  >  1:808659/1‑76 (MQ=25)
                                   ttCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATaa                                  >  2:3807067/1‑76 (MQ=25)
                                    tCGAATTGGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATaaa                                 <  2:1636685/76‑1 (MQ=25)
                                     cGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATaaaa                                >  1:3992593/1‑76 (MQ=25)
                                     cGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATaaaa                                >  1:993275/1‑76 (MQ=25)
                                     cGAATTGGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACTCCTTGCCTGACATaaaa                                <  2:2461398/76‑1 (MQ=21)
                                      gAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAg                               <  2:5812444/76‑1 (MQ=25)
                                      gAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAg                               <  1:1774875/76‑1 (MQ=25)
                                      gAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAg                               <  2:3034182/76‑1 (MQ=25)
                                      gAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAg                               >  2:4418008/1‑76 (MQ=25)
                                      gAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAg                               >  1:887134/1‑76 (MQ=25)
                                       gaTTTGCACGAAATCGGAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGt                              <  2:5795319/75‑1 (MQ=25)
                                       aaTTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCCACGAACGCCTTGCCTGACATAAAAGt                              >  2:2270122/1‑76 (MQ=25)
                                        aTTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTg                             <  2:4519454/76‑1 (MQ=25)
                                        aTTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTg                             >  2:791977/1‑76 (MQ=25)
                                        aTTTGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTg                             >  1:2559564/1‑76 (MQ=25)
                                          ttGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGcc                           <  2:2013258/76‑1 (MQ=25)
                                          ttGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGcc                           >  2:5370282/1‑76 (MQ=25)
                                           tGCCCGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAGCGAACGCCTTGCCTGACATAAAATTGCCg                          <  2:3684383/76‑1 (MQ=21)
                                           tGCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCg                          <  2:3431031/76‑1 (MQ=25)
                                            gCACGAAGTCGGGGGGCAGAGAAGGGGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCgg                         <  2:26633/76‑1 (MQ=25)
                                            gCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCgg                         >  2:5643258/1‑76 (MQ=25)
                                            gCACGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCgg                         >  2:5681095/1‑76 (MQ=25)
                                              aCGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgag                       <  2:2636408/76‑1 (MQ=25)
                                              aCGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgag                       >  2:599412/1‑76 (MQ=25)
                                              aCGAAATCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGgag                       <  1:5153438/76‑1 (MQ=25)
                                                    tCGTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtc                 <  2:2022740/76‑1 (MQ=25)
                                                      gTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtctc               <  1:2555853/76‑1 (MQ=37)
                                                      gTAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAtctc               <  2:1829019/76‑1 (MQ=37)
                                                       tggggCAGAGAAGGCGTTGA‑‑CG‑CGGCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATAGCTcc              <  2:5755673/74‑1 (MQ=25)
                                                       tAGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTcc              <  1:1739053/76‑1 (MQ=37)
                                                        gggggAGAGAAGGCGTCCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCg             <  2:5633877/75‑1 (MQ=37)
                                                        aGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCg             <  1:4632269/76‑1 (MQ=37)
                                                        aGGGCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCg             <  2:4727946/76‑1 (MQ=37)
                                                         gggCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCgg            >  1:1012602/1‑76 (MQ=37)
                                                          gggaGATGAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGc           <  2:2012233/73‑1 (MQ=25)
                                                           gCAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCa          <  2:2559564/76‑1 (MQ=37)
                                                            cAGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAt         >  1:3477938/1‑76 (MQ=37)
                                                             aGATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAtt        <  1:2216826/76‑1 (MQ=37)
                                                              gATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAttt       >  1:3607946/1‑76 (MQ=38)
                                                              gATAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAttt       >  1:5798329/1‑76 (MQ=38)
                                                               aTAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAtttt      <  2:1052781/76‑1 (MQ=37)
                                                                tAAGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCAttttt     >  1:3346194/1‑76 (MQ=38)
                                                                  aGGCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATCTCTCCGGCATTTTTAt   <  2:1261701/76‑1 (MQ=38)
                                                                   ggCGTTCACGC‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTAtt  >  1:4475826/1‑76 (MQ=38)
                                                                           ||                                                                     
GGCAAAAAACGTTAAACGCCCGTACTGGCCTACGGTTCGAATTTGCACGAAATCGTAGGGCAGATAAGGCGTTCA‑‑C‑‑C‑GCATCTGGCAACGAACGCCTTGCCTGACATAAAAGTGCCGGAGAATATCTCCGGCATTTTTATT  >  NC_000913/3537330‑3537470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A