Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F999 I1 R1	225	156.4	11709729	83.8%	9812752	75.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,686,775	+T	intergenic (‑23/+9)	b3530 ← / ← yhjM	putative oxidoreductase subunit/putative endoglucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,686,775	1	.	T	98.8%	529.1 / ‑0.5	166	intergenic (‑23/+9)	b3530/yhjM	putative oxidoreductase subunit/putative endoglucanase
Reads supporting (aligned to +/- strand): ref base . (2/0); new base T (76/86); total (80/86)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 174 total aligned reads displayed.

ACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAA  >  NC_000913/3686700‑3686843

aCTTACTCCAGAATCTGTTCCTGAGCGGTCGGTGCCACGTGGACCATCGGCATGACGGCCAGTCCGAGGGAAAGC‑g                                                                       <  1:4869047/76‑1 (MQ=255)

 cTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gt                                                                      <  1:4597423/76‑2 (MQ=255)

  ttGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtg                                                                     >  2:2272529/1‑74 (MQ=255)

  ttGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtg                                                                     >  1:4330866/1‑74 (MQ=255)

   tGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtga                                                                    >  1:5781045/1‑73 (MQ=255)

   tGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtga                                                                    <  2:2391060/76‑4 (MQ=255)

      tCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAata                                                                 >  2:4128094/1‑76 (MQ=255)

      tCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAata                                                                 >  1:2596544/1‑76 (MQ=255)

      tCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAata                                                                 >  2:5211316/1‑76 (MQ=255)

       ccAGCAACTGTTGCTGGGCGGTGGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAatat                                                                <  2:2174193/76‑1 (MQ=255)

       ccAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAatat                                                                >  1:1473734/1‑76 (MQ=255)

       ccAGCAACTGTTGCTGAGCGGGTGGTGCTGCCGCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GAGAatat                                                                >  2:2432256/1‑76 (MQ=255)

         aGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGt                                                              <  2:4258897/76‑1 (MQ=255)

         aGCAACGGTGGCTGGGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGt                                                              <  2:2532502/76‑1 (MQ=255)

          gCATCTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGtt                                                             <  1:5020464/76‑1 (MQ=255)

          gCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGtt                                                             <  1:3220206/76‑1 (MQ=255)

          gCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGtt                                                             >  2:2085655/1‑76 (MQ=255)

          gCAACTCTTGCTGAGCGGATGGTGCTGAATCGACCATCGGCAAGACCGCCAGAACGCGGGAAATC‑GTGTATATGtt                                                             >  1:3564422/1‑76 (MQ=255)

          gCAACGGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGtt                                                             <  2:3528819/76‑1 (MQ=255)

           cAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGttt                                                            >  1:3298057/1‑76 (MQ=255)

           cAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGttt                                                            >  1:4106399/1‑76 (MQ=255)

            aaCTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTa                                                           >  2:3641974/1‑76 (MQ=255)

             aCTGTTGCTCAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAg                                                          >  1:2576740/1‑76 (MQ=255)

              cTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAgt                                                         <  2:1862412/76‑1 (MQ=255)

               tGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAgtg                                                        <  2:3751088/76‑1 (MQ=255)

                gTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAgtgt                                                       <  1:971552/76‑1 (MQ=255)

                  tGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGa                                                     >  1:2177795/1‑76 (MQ=255)

                  tGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGa                                                     <  1:4621605/76‑1 (MQ=255)

                   gCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGaa                                                    >  1:951874/1‑76 (MQ=255)

                     tGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGACAGC‑GTGACTATGTTTAGCGCGAAtt                                                  >  2:729247/1‑76 (MQ=255)

                      gAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAAttt                                                 <  1:3845251/76‑1 (MQ=255)

                      gAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACAGAGGGAAAGC‑GTGAATATGTTTAGTGTGAAttt                                                 >  1:1251407/1‑76 (MQ=255)

                       cgCGGTTGGTACTGCCTCGTGCGTCGGGCTTACTTCCAGACCGAGGGAAAGC‑GTGACTATGTTTCGTGTGAGTTTg                                                <  2:5024291/75‑1 (MQ=255)

                       aGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTg                                                >  1:1093022/1‑76 (MQ=255)

                        gCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTgc                                               >  2:5315660/1‑76 (MQ=255)

                        gCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTgc                                               <  1:3139486/76‑1 (MQ=255)

                         cGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTgcg                                              <  1:289281/76‑1 (MQ=255)

                          ggTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTgcgn                                             <  1:4412167/76‑2 (MQ=255)

                          ggTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTgcgc                                             <  1:2262145/76‑1 (MQ=255)

                           gTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCa                                            <  2:3026199/76‑1 (MQ=255)

                            ttGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGCGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCAt                                           >  2:3367712/1‑76 (MQ=255)

                            ttGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCAt                                           >  2:3328281/1‑76 (MQ=255)

                              ggTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTc                                         <  1:2149124/76‑1 (MQ=255)

                              ggTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTc                                         <  1:1125095/76‑1 (MQ=255)

                               gTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTcc                                        <  2:1685039/76‑1 (MQ=255)

                                tgctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCt                                       >  1:514030/1‑76 (MQ=255)

                                 gctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTg                                      <  1:2837618/76‑1 (MQ=255)

                                 gctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTg                                      <  2:4478102/76‑1 (MQ=255)

                                  ctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTgg                                     <  1:5211317/76‑1 (MQ=255)

                                   tgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGc                                    >  1:4562278/1‑76 (MQ=255)

                                   tgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGc                                    <  2:2092603/76‑1 (MQ=255)

                                   tgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGc                                    >  1:2775912/1‑76 (MQ=255)

                                     ccTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGccc                                  <  1:5586197/76‑1 (MQ=255)

                                     ccTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGccc                                  >  2:4037417/1‑76 (MQ=255)

                                      cTCGACCATCGTCATGACGGCCATACCTAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGcccc                                 <  1:3450655/76‑1 (MQ=255)

                                      cTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGcccc                                 >  2:5674109/1‑76 (MQ=255)

                                       tCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCa                                >  1:1814296/1‑76 (MQ=255)

                                       tCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCa                                >  1:3981460/1‑76 (MQ=255)

                                        cGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAg                               >  2:126227/1‑76 (MQ=255)

                                         gACCATCGGCATGACGGCCAGACCGAGGGAGAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGt                              <  2:3653242/76‑1 (MQ=255)

                                         gACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAACTTGCGCATTCCTGGCCCCAgc                              >  2:4175215/1‑75 (MQ=255)

                                          aCCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTc                             >  1:2730653/1‑76 (MQ=255)

                                           ccATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCa                            <  1:3519289/76‑1 (MQ=255)

                                             aTCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAgg                          <  2:201555/76‑1 (MQ=255)

                                             aTCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAgg                          <  2:2699243/76‑1 (MQ=255)

                                              tCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGt                         >  1:751042/1‑76 (MQ=255)

                                              tCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGt                         <  2:2730653/76‑1 (MQ=255)

                                               cGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGta                        <  2:2438509/76‑1 (MQ=255)

                                                ggCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaa                       >  2:1441266/1‑76 (MQ=255)

                                                  cATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaata                     >  2:2859019/1‑76 (MQ=255)

                                                   aTGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaataa                    >  2:2622476/1‑76 (MQ=255)

                                                   aTGACGGCCAGACAGAGAGAAAGCGG‑GAATTTGTTTCGTGTGAATTTGCGCATTCCGGGCTCCAGGCAGGtaataa                    >  1:1101790/1‑76 (MQ=255)

                                                    tgccGGCCAGACCGAGGGAGAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAAc                   <  1:478721/73‑1 (MQ=255)

                                                     gACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACt                  >  1:2325213/1‑76 (MQ=255)

                                                     gACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACt                  >  1:659173/1‑76 (MQ=255)

                                                      aCGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTc                 <  1:68846/76‑1 (MQ=255)

                                                        ggCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAc               >  1:377912/1‑76 (MQ=255)

                                                        ggCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAc               >  2:4358136/1‑76 (MQ=255)

                                                        ggCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAc               >  2:3615477/1‑76 (MQ=255)

                                                        ggCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAc               <  1:4352829/76‑1 (MQ=255)

                                                         gCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAcc              >  1:1852963/1‑76 (MQ=255)

                                                         gCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAcc              <  2:2012965/76‑1 (MQ=255)

                                                          ccAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCt             <  2:3299396/76‑1 (MQ=255)

                                                          ccAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCt             >  2:2172097/1‑76 (MQ=255)

                                                          ccAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCt             <  2:1473734/76‑1 (MQ=255)

                                                           cAGACCGAGGGAAAGC‑GTGAATATGCTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCtt            <  2:3593545/76‑1 (MQ=255)

                                                            aGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCttt           >  1:2111698/1‑76 (MQ=255)

                                                            aGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCttt           <  1:83345/76‑1 (MQ=255)

                                                              aCCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTg         <  1:4683440/76‑1 (MQ=255)

                                                              aCCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTg         >  2:4941421/1‑76 (MQ=255)

                                                               ccGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGt        <  2:84753/76‑1 (MQ=255)

                                                                 gAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCg      <  2:4553660/76‑1 (MQ=255)

                                                                 gAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCg      <  2:3702658/76‑1 (MQ=255)

                                                                  aGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCga     <  1:5315661/76‑1 (MQ=255)

                                                                   gggAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCgag    <  2:4667693/76‑1 (MQ=255)

                                                                    ggAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCgaga   >  1:3835430/1‑76 (MQ=255)

                                                                     gAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAGTAACTCACCTTTTGTCGAGaa  >  1:5001070/1‑76 (MQ=255)

                                                                     gAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCgagca  >  2:5423229/1‑74 (MQ=255)

                                                                     gAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGaa  <  1:3003011/76‑1 (MQ=255)

                                                                     gAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGaa  <  2:568375/76‑1 (MQ=255)

ACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAA  >  NC_000913/3686700‑3686843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A