Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F999 I1 R1
|
226 |
132.7 |
9745491 |
85.7% |
8351885 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,737,956 |
2 bp→CG |
coding (623‑624/1254 nt) |
avtA → |
alanine‑alpha‑ketoisovalerate transaminase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,737,956 | 0 | G | C | 100.0%
| 286.0
/ NA
| 108 | G208A (GGC→GCG) | avtA | alanine‑alpha‑ketoisovalerate transaminase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (57/51); total (57/51) |
| * | NC_000913 | 3,737,957 | 0 | C | G | 99.1%
| 285.9
/ NA
| 108 | G208A (GGC→GCG) | avtA | alanine‑alpha‑ketoisovalerate transaminase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base G (56/51); total (57/51) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 108 total aligned reads displayed.
CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAG > NC_000913/3737888‑3738025
||
ctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGACGAGTTGCTGAAGCTTGACGCGCTGGCGAATCaa > 2:3563621/1‑76 (MQ=255)
ctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCaa > 1:425040/1‑76 (MQ=255)
ctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCaa > 1:4172929/1‑76 (MQ=255)
tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac > 2:4266951/1‑76 (MQ=255)
tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac < 2:3407938/76‑1 (MQ=255)
tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac < 2:3404354/76‑1 (MQ=255)
tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac < 2:2706793/76‑1 (MQ=255)
tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac < 2:2696997/76‑1 (MQ=255)
cccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAaca > 2:4414528/1‑76 (MQ=255)
ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac < 1:4533403/76‑1 (MQ=255)
ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac > 1:4817555/1‑76 (MQ=255)
ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac < 1:1907016/76‑1 (MQ=255)
ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac < 2:4050565/76‑1 (MQ=255)
cGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACg > 2:2155064/1‑76 (MQ=255)
cGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACg < 1:3980403/76‑1 (MQ=255)
gCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGc < 2:4812497/76‑1 (MQ=255)
gCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGc < 1:4440374/76‑1 (MQ=255)
gCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGc < 2:3574822/76‑1 (MQ=255)
ccGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCa < 1:3620751/76‑1 (MQ=255)
cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTTCTGAAGCTTGACGCCCCTGCCAACCCACACCGCAt > 2:2388758/1‑76 (MQ=255)
cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGGTGAAGCTTGACGCGCTGGCGAATCAACACGGCAt > 1:3340659/1‑76 (MQ=255)
cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAt > 2:3683928/1‑76 (MQ=255)
gacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAtt > 1:1180075/1‑76 (MQ=255)
gacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAtt > 1:349862/1‑76 (MQ=255)
acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATTAACACGGCATTc > 2:563244/1‑76 (MQ=255)
acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTc < 2:4481266/76‑1 (MQ=255)
acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTc < 1:55066/76‑1 (MQ=255)
cgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTcc > 1:2410654/1‑76 (MQ=255)
cgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTcc > 1:2456827/1‑76 (MQ=255)
gaATCCAACAGTGAATGTGATTACTGACGAAGAGTTGCTGCAGCTTGACGCGCTGGCGAATCCACACGGCATTCCg < 2:2251878/76‑1 (MQ=255)
gaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCg > 2:2832678/1‑76 (MQ=255)
aaTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTTCGc < 1:3110223/76‑1 (MQ=255)
aaTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGc > 2:3641723/1‑76 (MQ=255)
aaTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCGTGACGCGCTGGCGTATCAACACGGCGTTTCGc > 1:4843719/1‑76 (MQ=255)
aTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCt > 1:4400828/1‑76 (MQ=255)
accAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTg < 2:161956/75‑1 (MQ=255)
cAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGt < 2:402074/76‑1 (MQ=255)
aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtg < 2:4752813/76‑1 (MQ=255)
aaCAGGCAAGGAGATTACTGACGAAGGGTTGCTGAAGCTTGACGCGCTCGCGTATCAACACGGCAGTCCGCTgggg > 1:3662392/1‑74 (MQ=255)
aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtga > 2:1733170/1‑76 (MQ=255)
aGGCAATGTGATTTCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgatt < 2:1503094/76‑1 (MQ=255)
aGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgatt > 1:2727449/1‑76 (MQ=255)
aGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgatt < 2:474668/76‑1 (MQ=255)
aGGCAAGGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgatt < 2:776630/76‑1 (MQ=255)
gCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattga > 1:1852234/1‑76 (MQ=255)
gCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattga < 2:4521778/76‑1 (MQ=255)
cAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattgat > 2:935741/1‑76 (MQ=255)
cAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattgat < 1:3852041/76‑1 (MQ=255)
aaTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATa > 1:3999752/1‑76 (MQ=255)
aaTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATa > 1:3174051/1‑76 (MQ=255)
aTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATaa > 1:217296/1‑76 (MQ=255)
tgtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAc < 1:4335032/76‑1 (MQ=255)
tgtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAc > 2:1317490/1‑76 (MQ=255)
gtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACg > 1:2225284/1‑76 (MQ=255)
tgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGc < 2:2803859/76‑1 (MQ=255)
gATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCt > 2:3325662/1‑76 (MQ=255)
gATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCt > 1:4480463/1‑76 (MQ=255)
gATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGCGATTGATAACGCt > 2:119782/1‑76 (MQ=255)
aTTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCtt < 1:1518895/76‑1 (MQ=255)
aTTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATCGATAACGCtt < 2:425040/76‑1 (MQ=255)
ttACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa < 1:2261566/76‑1 (MQ=255)
tACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTag > 2:4666534/1‑75 (MQ=255)
aCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATg > 2:1820091/1‑76 (MQ=255)
aCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATg < 1:2652358/76‑1 (MQ=255)
cTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg < 2:1602392/76‑1 (MQ=255)
gACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCg > 2:4371347/1‑76 (MQ=255)
gACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCg > 1:62389/1‑76 (MQ=255)
gcGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGt < 2:845655/75‑1 (MQ=255)
aCGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGt > 2:1870765/1‑76 (MQ=255)
aCGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGt > 2:4795568/1‑76 (MQ=255)
aCGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGt > 2:1527495/1‑76 (MQ=255)
cGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTc < 1:789550/76‑1 (MQ=255)
cGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTc > 2:3758826/1‑76 (MQ=255)
gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTcc < 1:4794512/76‑1 (MQ=255)
gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTcc > 2:4150801/1‑76 (MQ=255)
aaGAGTTGCTGAACCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGAGTATGCTCATGGCATccc > 1:315670/1‑76 (MQ=255)
agagTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCg < 1:479416/76‑1 (MQ=255)
gagTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGt < 1:2388759/76‑1 (MQ=255)
gagTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCCTcccct > 2:10497/1‑74 (MQ=255)
agTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGtt > 1:3276121/1‑76 (MQ=255)
gTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTc < 1:4084754/76‑1 (MQ=255)
gTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTc < 2:392764/76‑1 (MQ=255)
gTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTc > 1:703734/1‑76 (MQ=255)
ttGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTcc > 1:4702414/1‑76 (MQ=255)
ttGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTcc > 1:3770764/1‑76 (MQ=255)
tGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTccc < 1:3497194/76‑1 (MQ=255)
gCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCg < 2:3055524/76‑1 (MQ=255)
gCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCg < 2:1371149/76‑1 (MQ=255)
gCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCg > 1:545900/1‑76 (MQ=255)
cTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCgg < 2:3229760/76‑1 (MQ=255)
aaGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGCTCCCGGCTa > 2:673415/1‑76 (MQ=255)
aGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCCGGTGATTGATAACGCTTATGGCGTCCCGTTCCAGGGTat > 1:502411/1‑76 (MQ=255)
gCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatc < 1:2289120/76‑1 (MQ=255)
cTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatca > 2:342698/1‑76 (MQ=255)
ttGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcat > 1:3522415/1‑76 (MQ=255)
gtGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcat < 1:161575/75‑1 (MQ=255)
gcgcgCTGGCGAATCAACCCGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCtt < 1:4349804/75‑1 (MQ=255)
cgcgCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTc > 2:28110/1‑76 (MQ=255)
cgcgCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTc < 1:1840832/76‑1 (MQ=255)
gcgcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCa > 2:2677267/1‑76 (MQ=255)
||
CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAG > NC_000913/3737888‑3738025
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
