isobutanol-resistant Ecoli: JCL260__isobutanol_production_host-M9-MG1655gbk
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Metadata |
Mutations |
Converged Mutations |
Fixed Mutations |
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Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A2 F999 I1 R1 | 226 | 132.7 | 9745491 | 85.7% | 8351885 | 75.9 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,945,351 | +C | coding (1/600 nt) coding (240/240 nt) |
yifDA ← yifDA ← |
transcription regulator transcription regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,945,350 | 1 | . | C | 99.2% | 315.8 / ‑4.5 | 120 | E81G (GAG→GGG) | yifDA | transcription regulator |
| Reads supporting (aligned to +/- strand): ref base . (0/0); major base C (47/72); minor base A (0/1); total (47/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
ATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTT > NC_000913/3945277‑3945420 | ttAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct‑cct < 1:128611/76‑3 (MQ=255) tAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACct‑cctt > 1:3330044/1‑73 (MQ=255) gCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACg < 2:4756503/76‑1 (MQ=255) gCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACg > 2:343863/1‑76 (MQ=255) gCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACg < 2:4108237/76‑1 (MQ=255) cATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACgg < 2:4240091/76‑1 (MQ=255) cATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACgg < 1:3486927/76‑1 (MQ=255) cATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACgg > 1:3090610/1‑76 (MQ=255) aTACATTCTCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACggg < 1:973640/76‑1 (MQ=255) aTACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACggg > 1:1945967/1‑76 (MQ=255) tACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGggc < 1:3885798/76‑1 (MQ=255) aaCATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGggc < 2:83120/75‑1 (MQ=255) aCATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGggcg < 1:2746280/76‑1 (MQ=255) aTTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGggcggc > 2:2466916/1‑76 (MQ=255) tCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCt < 2:2439300/76‑1 (MQ=255) tCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCt > 1:3478471/1‑76 (MQ=255) tCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCt < 2:2462296/76‑1 (MQ=255) cccACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTg < 1:2405572/76‑1 (MQ=255) cacaACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGcc < 1:3906549/76‑1 (MQ=255) cacaACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGcc > 2:2277070/1‑76 (MQ=255) acaACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCa < 1:3136293/76‑1 (MQ=255) caACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAg > 1:2643651/1‑76 (MQ=255) aCGAGGCGCTGGCCCCGATAGAAAACTCGTTATGTCGTGAGGTAGGCGCCACCTCCTTACGGGCGGCCTGCCAGGt < 2:1652705/76‑1 (MQ=255) aCGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAggg > 2:3914075/1‑75 (MQ=255) cGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTg > 2:3667979/1‑76 (MQ=255) cGAGGCGCTCGCACCGATAGAAAACTCGTTATGGCGTGAGTGATGCGCCACCTACTTACGGGCGGCCTGCCAGGTg < 2:2317128/76‑1 (MQ=255) cGAGGCGCTAGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTg < 2:2313224/76‑1 (MQ=255) gAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCCGgcgc > 2:3281424/1‑76 (MQ=255) gAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGc < 2:2602830/76‑1 (MQ=255) gAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGc > 1:2965601/1‑76 (MQ=255) ggCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTc < 2:672135/76‑1 (MQ=255) gcgcTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCa > 2:2115510/1‑76 (MQ=255) gcgcTGGCACCGATAGAAAACTAGTTATGTCGTGAGGTATGCGCAACCTCCTTACGGGCGACGTGCCAGGTGCtat > 2:3073681/1‑74 (MQ=255) cgcTGGCCCCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCAt < 2:1370191/76‑1 (MQ=255) cgcTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCAt < 1:1614144/76‑1 (MQ=255) cTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGa < 2:1792866/76‑1 (MQ=255) cTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGa < 1:2154090/76‑1 (MQ=255) ggCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGc < 1:3549097/76‑1 (MQ=255) gCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCg > 2:1130048/1‑76 (MQ=255) cACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGt > 1:4616040/1‑76 (MQ=255) aCCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCCGGTGCTCATGAGCGtt > 2:4449676/1‑76 (MQ=255) aCCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGtt > 1:159080/1‑76 (MQ=255) aCCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGtt > 1:2053126/1‑76 (MQ=255) ccGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGttt < 2:1390793/76‑1 (MQ=255) gATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCt < 1:3215732/76‑1 (MQ=255) gATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCt > 2:4794571/1‑76 (MQ=255) caTAGAAAACTCGTTATGTCGTGAGGTATGCGCCCCCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCt < 1:4590102/75‑1 (MQ=255) aTAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGATCGTTTCTg > 1:2730615/1‑76 (MQ=255) tAGAGAGCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGc < 2:2928869/76‑1 (MQ=255) tAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTCCGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGc < 1:3851575/76‑1 (MQ=255) aGAAAACTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGCGCGGCCTGCCAGCTGCTCATGATCGTTTCtcct > 2:1144968/1‑73 (MQ=255) aaaaCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATa < 1:690858/76‑1 (MQ=255) aaaaCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATa < 1:2346609/76‑1 (MQ=255) aaaaCTAGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATa < 1:2365144/76‑1 (MQ=255) aaaCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATaa > 2:3569405/1‑76 (MQ=255) aCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAgg < 2:2533601/76‑1 (MQ=255) aCTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAgg < 2:4408974/76‑1 (MQ=255) cTCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAAGTGCTCATGAGCGTTTCTGCATAAGGc < 1:1371667/76‑1 (MQ=255) tCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGCGCGTTTCTGCATACGGCa > 2:3148817/1‑76 (MQ=255) tCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCa > 1:3974605/1‑76 (MQ=255) tCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCa < 1:1924707/76‑1 (MQ=255) tCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCa < 1:2059529/76‑1 (MQ=255) tCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCa > 2:3122860/1‑76 (MQ=255) tCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCa > 2:3462935/1‑76 (MQ=255) tCGTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCa < 1:621923/76‑1 (MQ=255) gtgaTGTCGTGAGGTATGCGCCCCCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagt < 2:181842/73‑1 (MQ=255) gTTATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagt < 1:4599041/76‑1 (MQ=255) tATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagtag > 1:1251143/1‑76 (MQ=255) tATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagtag < 2:3784595/76‑1 (MQ=255) tATGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagtag > 2:2432076/1‑76 (MQ=255) aTGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCagtagt > 1:3312956/1‑76 (MQ=255) tGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATACGGCAGTAGtt > 2:4599201/1‑76 (MQ=255) tGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGtt < 2:3312956/76‑1 (MQ=255) tGTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGtt < 2:3814861/76‑1 (MQ=255) gTCGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttt < 1:3169931/76‑1 (MQ=255) gTCGGGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttt < 2:268599/76‑1 (MQ=255) tCGTGAGGTATGCCCCCCCTCCTGACGGGCGGCCTGCTAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGtttt < 1:3073681/76‑1 (MQ=255) cGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttttt < 2:2826120/76‑1 (MQ=255) cGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttttt > 2:709234/1‑76 (MQ=255) cGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGttttt < 1:3462935/76‑1 (MQ=255) cGTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCGCATGAGCGTTTCTGAATAAGGCAGGAGttttt > 2:2838019/1‑76 (MQ=255) gTGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTc < 1:4683493/76‑1 (MQ=255) tGAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCa < 2:2741671/76‑1 (MQ=255) gAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCAc > 1:488411/1‑76 (MQ=255) gAGGTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCAc < 2:1626888/76‑1 (MQ=255) ggTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCg < 2:3877678/76‑1 (MQ=255) ggTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCg < 1:3293037/76‑1 (MQ=255) ggTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCg > 1:3357529/1‑76 (MQ=255) gTATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCgg > 1:3990937/1‑76 (MQ=255) tATGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGc < 1:2670243/76‑1 (MQ=255) aTGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCa < 2:2421778/76‑1 (MQ=255) tGCGCCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAg > 2:600804/1‑76 (MQ=255) gcgcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGc < 1:1106460/76‑1 (MQ=255) gcgcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGc < 1:2432226/76‑1 (MQ=255) cgcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCg > 2:1337632/1‑76 (MQ=255) gcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCCTAAGGCAGTAGTTTTTCACCGGCAGCgg > 2:470717/1‑76 (MQ=255) gcCACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCgg > 1:1517204/1‑76 (MQ=255) cACCTCCTTACGGGCGTCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGtt < 1:2970112/76‑1 (MQ=255) cACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGtt > 2:4512262/1‑76 (MQ=255) cACCTCCTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGtt < 2:2520701/76‑1 (MQ=255) | ATTAAGCATACATTCCCACAACGAGGCGCTGGCACCGATAGAAAACTCGTTATGTCGTGAGGTATGCGCCACCT‑CTTACGGGCGGCCTGCCAGGTGCTCATGAGCGTTTCTGCATAAGGCAGTAGTTTTTCACCGGCAGCGGTT > NC_000913/3945277‑3945420 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |