Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F999 I1 R1
|
225 |
156.4 |
11709729 |
83.8% |
9812752 |
75.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,950,557 |
2 bp→GC |
coding (451‑452/930 nt) |
ilvE → |
branched‑chain amino‑acid aminotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,950,557 | 0 | C | G | 99.3%
| 360.2
/ NA
| 138 | R151A (CGA→GCA) | ilvE | branched‑chain amino‑acid aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base G (68/69); total (68/70) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| * | NC_000913 | 3,950,558 | 0 | G | C | 100.0%
| 368.7
/ NA
| 139 | R151A (CGA→GCA) | ilvE | branched‑chain amino‑acid aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (68/71); total (68/71) |
Only 100 of 139 total aligned reads displayed.
GAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG > NC_000913/3950489‑3950626
||
aGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAac > 1:2875141/1‑76 (MQ=255)
gCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAaca > 1:1070187/1‑76 (MQ=255)
gCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAaca > 1:4186154/1‑76 (MQ=255)
cGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAacac < 2:2622121/76‑1 (MQ=255)
gTATCTGGGCGCAGCAGCGATGGAGCAGGGGATCGCTGCGAAGGCTCCCTCCTGGAACCGCGCTGCACCAAACAcc > 1:137469/1‑76 (MQ=255)
gTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAcc > 1:4136676/1‑76 (MQ=255)
aTCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAt > 2:4708520/1‑76 (MQ=255)
cTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATcc < 2:1699091/76‑1 (MQ=255)
tGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATccc < 2:3376113/76‑1 (MQ=255)
ggggCGCACAAGCGCTGGAGCAGGGGATCGATGCGATAGTTTCCGCCTGGAACCGCGCAGCACCAAACACCATccc > 2:4916669/2‑76 (MQ=255)
gggCGTAGAGGTGCTGGAGCAGGGGCGCGAGGCGAGGGATTCCGCCTGGAAGCGCGCAGCACCACACACCATCCCg > 1:5340750/1‑76 (MQ=255)
gggCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCg > 1:1187968/1‑76 (MQ=255)
gggCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCg > 1:1676601/1‑76 (MQ=255)
gcgcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAc < 2:1070187/76‑1 (MQ=255)
gcgcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAc < 1:5577654/76‑1 (MQ=255)
gcgcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAc < 1:4893701/76‑1 (MQ=255)
cAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggc < 1:1549442/76‑1 (MQ=255)
cAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggc > 2:1427335/1‑76 (MQ=255)
cAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAccgc > 2:668394/1‑76 (MQ=255)
cAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAACATCCCGAcggc > 1:4056430/1‑76 (MQ=255)
aGAAGGGCGGGAGCGGGGGAGCGATACGATGGTTTCCTCCGGGTACCGCGCCGCACTAATCACCATCCCGAcggcg < 2:255335/76‑1 (MQ=255)
aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggcg < 1:701215/76‑1 (MQ=255)
aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggcg < 1:758166/76‑1 (MQ=255)
aaGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACggcggc < 1:3219869/76‑1 (MQ=255)
aaGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACggcggc < 1:905840/76‑1 (MQ=255)
aGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACGAAACACCATCCCGACGGCGGCa < 2:4200960/76‑1 (MQ=255)
aGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCa < 2:1249025/76‑1 (MQ=255)
aGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCa > 1:471356/1‑76 (MQ=255)
aGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCa < 2:2413464/76‑1 (MQ=255)
aGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCa < 1:4105175/76‑1 (MQ=255)
aGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCa < 2:5595107/76‑1 (MQ=255)
cgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGTAACCTCGCAGCACCAACCGCCCTCCCGACGGCGGCaaa < 1:3344091/76‑1 (MQ=255)
cgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaa < 2:1279719/76‑1 (MQ=255)
cgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaa < 1:4326275/76‑1 (MQ=255)
cgcTGGAGCAGGGGATCCATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaa > 2:4165759/1‑76 (MQ=255)
gcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGATCCGCGCAGCACCATACACCATCCCGACGGCGGCaaaa > 1:70334/1‑76 (MQ=255)
gcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGCCGGCGGCcaaa > 2:434670/1‑76 (MQ=255)
gcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa < 1:4557089/76‑1 (MQ=255)
gcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa > 2:4977293/1‑76 (MQ=255)
gatGGAGCAGGGGATCGATGCGACGGTTTCCTCACGGAAACGAGCAGCACCAAACAACATCACGACGGCGGCgaaa > 1:3672151/3‑76 (MQ=255)
gtGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAg < 2:3249373/75‑1 (MQ=255)
cTGGGGCAGGGGATCGCTGCGAGGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAg < 2:4579805/76‑1 (MQ=255)
tGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGc > 1:2933464/1‑76 (MQ=255)
ggAGCGGGGGATCGATGCGATGCTTTCCTCCTGGAACGGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGcc < 1:2864763/76‑1 (MQ=255)
gAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCg > 2:3503144/1‑76 (MQ=255)
gAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCg < 2:1532813/76‑1 (MQ=255)
aGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCCAACGCCgg > 2:1678607/1‑76 (MQ=255)
aGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCgg > 2:5267456/1‑76 (MQ=255)
aGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCgg < 1:5667518/76‑1 (MQ=255)
aGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCgg > 1:1191576/1‑76 (MQ=255)
gCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCCTCCCGACGGCGGCACAAGcccga > 2:5268375/1‑73 (MQ=255)
gCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggg > 1:488333/1‑75 (MQ=255)
aGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtgg < 1:2960973/76‑1 (MQ=255)
ggggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtggt < 2:1476762/76‑1 (MQ=255)
ggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGAGGTaa > 1:205707/1‑76 (MQ=255)
ggtCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc > 1:2499857/3‑76 (MQ=255)
gATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc > 2:146696/1‑76 (MQ=255)
aTCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTACCt > 2:5339766/1‑76 (MQ=255)
gcgatgcgaggGTTTCCGCCGGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTa < 2:5825976/75‑1 (MQ=255)
gatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAcc > 2:2161819/1‑76 (MQ=255)
gatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAcc > 2:3447301/1‑76 (MQ=255)
gcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctct > 1:5692688/1‑76 (MQ=255)
cgatgGTTTCCTCCTGGACCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctc < 1:1188328/76‑1 (MQ=255)
cgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctc > 1:5554904/1‑76 (MQ=255)
gatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctct > 1:1030149/1‑76 (MQ=255)
gatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctct < 2:4657115/76‑1 (MQ=255)
atgGTTTCCTCCTGGACCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCtt < 1:5713023/76‑1 (MQ=255)
tgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTc > 1:1106509/1‑76 (MQ=255)
tgGTGTCCTCCGGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTc < 2:2411207/76‑1 (MQ=255)
ggTTTCCTCCTGGAACGGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTcc < 2:4944518/76‑1 (MQ=255)
ttCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctg < 1:4921149/76‑1 (MQ=255)
ttCCTCCTGCAACGGCGAAGCACCAAAGACCAGCCCAACGGCGGCAAAAGCCGGTCGCAACGACCTCTCATCCctt > 2:2685861/1‑75 (MQ=255)
tcctccTGGACCCGCGCAGCACCAAACCCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgc < 1:722089/76‑1 (MQ=255)
tcctccTGGAACCGCGCAGCCCCCCACACCATCCCGACGGCGTCAAACGCCGGGGGTCACTACCTCTCTCCCctgc > 2:3829987/1‑76 (MQ=255)
tcctccTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgc > 2:3796664/1‑76 (MQ=255)
tcctccTGAACCCGCGCAGCACCAACCACCACCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgc < 1:2385450/76‑1 (MQ=255)
cctcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgct > 1:1296077/1‑76 (MQ=255)
cctcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgct > 1:3013382/1‑76 (MQ=255)
cctccgGGACCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgct < 1:2631637/76‑1 (MQ=255)
ccccctGGACCCACGCGCCGCCAAACCCCACCCCGACGGCGGCAAACGCCGGTGGTAACTACCTCTCTTCCctgct < 1:805057/73‑1 (MQ=255)
ctcctGGACCCGCGCAGCACCAAACCCCACCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgctg < 1:232277/76‑1 (MQ=255)
ctcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGGGGTACCTACCTCTCTTCCccgctg > 2:115976/1‑76 (MQ=255)
tcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtgg > 1:1339387/1‑76 (MQ=255)
ccgggACCCGCGCAGCACCAAACACCATCCCGCCGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggt < 2:3277353/73‑1 (MQ=255)
ctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc > 1:5324257/1‑55 (MQ=255)
ctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg > 1:4224142/1‑76 (MQ=255)
ctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg > 1:175388/1‑76 (MQ=255)
ctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg > 1:4460358/1‑76 (MQ=255)
ctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg > 2:4981491/1‑76 (MQ=255)
tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtgg < 1:5094298/76‑1 (MQ=255)
tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtgg < 1:4977294/76‑1 (MQ=255)
tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtgg > 1:5319174/1‑76 (MQ=255)
tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtgg > 1:2061122/1‑76 (MQ=255)
ggAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTggg > 1:3326412/1‑76 (MQ=255)
gacccGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGt < 1:1795667/73‑1 (MQ=255)
gAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGt > 2:3973329/1‑76 (MQ=255)
acccGCGCAGCACCAAACACCACCCCGCCGGCGGAAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa < 1:2710749/74‑1 (MQ=255)
aaCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa > 1:2449257/1‑76 (MQ=255)
cccGCGCAGCACCAAACCCCACCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAg < 2:5424066/75‑1 (MQ=255)
cgcgCAGCACCAAACACCACCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCg < 2:1522638/76‑1 (MQ=255)
||
GAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG > NC_000913/3950489‑3950626
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
