Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	715	38.4	1606192	96.6%	1551581	120.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,491,389	A→G	K163R (AAG→AGG)	cybB →	cytochrome b561

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,491,389	0	A	G	100.0%	129.8 / NA	36	K163R (AAG→AGG)	cybB	cytochrome b561
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (11/25); total (11/25)

CGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTATATTTAT  >  NC_000913/1491254‑1491522

cGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGa                                                                                                                                    <  1:789397/139‑1 (MQ=255)

   tGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGacaa                                                                                                                                 >  1:694001/1‑139 (MQ=255)

     ccTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGacaaca                                                                                                                               <  2:526074/139‑1 (MQ=255)

     ccTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGacaaca                                                                                                                               <  1:483583/139‑1 (MQ=255)

       ttACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAacaca                                                                                                                             <  2:271802/139‑1 (MQ=255)

               cAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACg                                                                                                                     >  2:439827/1‑139 (MQ=255)

                  agGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCat                                                                                                                  >  2:237726/1‑139 (MQ=255)

                            cGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATCATGCCGCGt                                                                                                        <  1:187040/139‑1 (MQ=255)

                               aCGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTaaa                                                                                                     <  2:630774/139‑1 (MQ=255)

                                      gATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCt                                                                                              <  2:359703/139‑1 (MQ=255)

                                           cTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAgg                                                                                         >  2:666146/1‑139 (MQ=255)

                                                aGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATAtt                                                                                    >  1:717273/1‑139 (MQ=255)

                                                   cGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTaa                                                                                 >  1:642506/1‑139 (MQ=255)

                                                            aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGc                                                                        >  2:666248/1‑139 (MQ=255)

                                                             aaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                       <  2:694001/139‑1 (MQ=255)

                                                             aaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                       <  1:316149/139‑1 (MQ=255)

                                                                          cTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCg                                                          <  2:261452/139‑1 (MQ=255)

                                                                                 atTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTg                                                   <  1:251713/139‑1 (MQ=255)

                                                                                    ttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                                <  1:242039/139‑1 (MQ=255)

                                                                                       tCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAg                                             >  1:432293/1‑139 (MQ=255)

                                                                                                 gCACGCTGCGGCTGCACTGGCACACCACTTTTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACTCGTGGTACAGCCGAAGAATa                                   <  2:542473/139‑1 (MQ=255)

                                                                                                 gCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                   <  2:120250/139‑1 (MQ=255)

                                                                                                       tGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                             <  1:460266/139‑1 (MQ=255)

                                                                                                       tGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGACGAAGAATACGGGTc                             <  2:666215/139‑1 (MQ=255)

                                                                                                           gCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACa                         <  1:684170/139‑1 (MQ=255)

                                                                                                                aCTGGGACACCACTGTTTCTGAAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGTATACGGGTCTACATCGGa                    <  1:183647/139‑1 (MQ=255)

                                                                                                                 cTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGaa                   <  1:287666/139‑1 (MQ=255)

                                                                                                                 cTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGaa                   >  1:205238/1‑139 (MQ=255)

                                                                                                                  tGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAg                  <  2:633711/139‑1 (MQ=255)

                                                                                                                   ggCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAgc                 <  2:642506/139‑1 (MQ=255)

                                                                                                                    gCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                   <  2:191015/120‑1 (MQ=255)

                                                                                                                    gCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                   >  1:191015/1‑120 (MQ=255)

                                                                                                                        accacTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCta            <  2:205238/139‑1 (MQ=255)

                                                                                                                        accacTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCta            <  1:237726/139‑1 (MQ=255)

                                                                                                                        accacTATTTCTGGAGGGACAACACACATCTACGCATGAGGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACTGGTCTACATCGGAAGCGCCta            <  2:89471/139‑1 (MQ=255)

                                                                                                                                  cTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTAtatttat  >  2:62426/1‑139 (MQ=255)

CGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTATATTTAT  >  NC_000913/1491254‑1491522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A