Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,491,389 |
A→G |
K163R (AAG→AGG) |
cybB → |
cytochrome b561 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,491,389 | 0 | A | G | 100.0%
| 187.3
/ NA
| 52 | K163R (AAG→AGG) | cybB | cytochrome b561 |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/28); total (24/28) |
CGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTATAT > NC_000913/1491254‑1491518
|
cGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGa < 2:64477/139‑1 (MQ=255)
gCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGacaac > 1:744606/1‑139 (MQ=255)
cAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACg > 1:238327/1‑139 (MQ=255)
gCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGa < 2:515142/119‑1 (MQ=255)
gCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGa > 1:515142/1‑119 (MQ=255)
tttCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCcg > 2:252936/1‑139 (MQ=255)
aCGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTaaa < 1:252936/139‑1 (MQ=255)
aCGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTaaa < 2:1127297/139‑1 (MQ=255)
aTAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTg < 2:129742/139‑1 (MQ=255)
tAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTa < 1:1096143/107‑1 (MQ=255)
tAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTa > 2:1096143/1‑107 (MQ=255)
tAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTaaa < 1:12655/125‑1 (MQ=255)
tAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTaaa > 2:12655/1‑125 (MQ=255)
tAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGat > 1:117728/1‑139 (MQ=255)
tAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGat > 1:374011/1‑139 (MQ=255)
tAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGat > 1:655456/1‑139 (MQ=255)
tAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGat > 1:687688/1‑139 (MQ=255)
aaaGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGata > 1:655457/1‑139 (MQ=255)
gCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGaa < 2:374011/139‑1 (MQ=255)
aaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc > 1:1072572/1‑139 (MQ=255)
aaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc < 2:770792/139‑1 (MQ=255)
cTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTaa > 2:953707/1‑104 (MQ=255)
cTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTaa < 1:953707/104‑1 (MQ=255)
tGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGacaa < 2:815143/76‑1 (MQ=255)
tGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGacaa > 1:815143/1‑76 (MQ=255)
tGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTAc > 2:475473/1‑139 (MQ=255)
ggCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCt < 2:375546/110‑1 (MQ=255)
ggCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCt > 1:375546/1‑110 (MQ=255)
tCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAAcc < 2:559830/139‑1 (MQ=255)
ggATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGAcc < 2:687688/139‑1 (MQ=255)
gATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCt < 2:445718/73‑1 (MQ=255)
gATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCt > 1:445718/1‑73 (MQ=255)
ttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa > 2:950840/1‑139 (MQ=255)
ttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa < 1:375859/139‑1 (MQ=255)
tCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAg > 1:782955/1‑139 (MQ=255)
tCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCg < 1:475473/139‑1 (MQ=255)
gCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa < 2:744606/139‑1 (MQ=255)
tGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc < 2:1072572/139‑1 (MQ=255)
gCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACa < 2:238327/139‑1 (MQ=255)
cTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGaa < 1:950840/139‑1 (MQ=255)
ggCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGa > 1:828321/1‑136 (MQ=255)
ggCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGa < 2:828321/136‑1 (MQ=255)
accacTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATGTTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCta < 1:916807/139‑1 (MQ=255)
cacTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCg < 2:574063/107‑1 (MQ=255)
cacTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCg > 1:574063/1‑107 (MQ=255)
tATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATtata < 1:354859/139‑1 (MQ=255)
aTTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCg < 2:1034572/87‑1 (MQ=255)
aTTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCg > 1:1034572/1‑87 (MQ=255)
aTTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc < 2:340462/116‑1 (MQ=255)
aTTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc > 1:340462/1‑116 (MQ=255)
aTTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTatat > 2:138233/1‑139 (MQ=255)
ggAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAGACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc < 2:422473/110‑1 (MQ=255)
|
CGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTATAT > NC_000913/1491254‑1491518
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
