Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
715 |
38.4 |
1606192 |
96.6% |
1551581 |
120.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,869,022 |
(T)6→5 |
coding (68/1284 nt) |
yeaH → |
UPF0229 family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,869,017 | 0 | T | . | 100.0%
| 119.6
/ NA
| 29 | coding (63/1284 nt) | yeaH | UPF0229 family protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (15/14); total (15/14) |
AGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTT > NC_000913/1868884‑1869145
|
aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta < 2:427011/139‑2 (MQ=255)
cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg < 2:185826/139‑4 (MQ=255)
cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg < 1:708340/139‑4 (MQ=255)
gAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacgc < 1:310420/139‑5 (MQ=255)
agaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgc > 2:693037/1‑136 (MQ=255)
agaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgc < 1:693037/136‑1 (MQ=255)
tAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc < 1:489494/139‑1 (MQ=255)
aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg < 1:532303/139‑1 (MQ=255)
tGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaa < 2:676763/139‑1 (MQ=255)
ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat > 2:133952/1‑139 (MQ=255)
tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTc < 1:310726/139‑1 (MQ=255)
taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGt > 2:301823/1‑139 (MQ=255)
cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga > 2:434156/1‑139 (MQ=255)
cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCG‑TCTCCGAGGCCATTAATAAGCGTTCGGtgac < 1:691263/139‑1 (MQ=255)
gggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg > 2:401361/1‑114 (MQ=255)
gggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg < 1:401361/114‑1 (MQ=255)
ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac > 2:255047/1‑139 (MQ=255)
cTGGTTTATTGACCGGCGTCTGACCGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcg > 1:441246/1‑139 (MQ=255)
ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa < 1:726699/93‑1 (MQ=255)
ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa > 2:726699/1‑93 (MQ=255)
gACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGt > 1:540625/1‑139 (MQ=255)
ccGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGc > 2:729492/1‑92 (MQ=255)
ccGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGc < 1:729492/92‑1 (MQ=255)
cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc > 2:622102/1‑139 (MQ=255)
gCGTCTGAACGGCAAAAACAAAAGCATGGTGAAGCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCa < 2:441246/139‑1 (MQ=255)
aCGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg < 1:113219/139‑1 (MQ=255)
gCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgc > 2:476330/1‑51 (MQ=255)
gCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgc < 1:476330/51‑1 (MQ=255)
tGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat > 2:308930/1‑70 (MQ=255)
tGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat < 1:308930/70‑1 (MQ=255)
ggTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGa > 1:28519/1‑139 (MQ=255)
gAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGt < 2:28519/139‑1 (MQ=255)
aaTCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTACCACGGAAGATATTAGCGAACCGATGtt > 2:479075/1‑139 (MQ=255)
|
AGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTT > NC_000913/1868884‑1869145
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
