Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I1 R1
|
776 |
60.0 |
3074904 |
90.1% |
2770488 |
104.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,869,022 |
(T)6→5 |
coding (68/1284 nt) |
yeaH → |
UPF0229 family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,869,017 | 0 | T | . | 100.0%
| 136.3
/ NA
| 33 | coding (63/1284 nt) | yeaH | UPF0229 family protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (15/18); total (15/18) |
ACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGA > NC_000913/1868882‑1869141
|
aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttt > 2:725149/1‑139 (MQ=255)
aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttt > 2:357802/1‑139 (MQ=255)
aGCGAACCGTGGGCCTGAGAAGCGACAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta < 1:855865/139‑2 (MQ=255)
cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg < 2:1480718/139‑4 (MQ=255)
cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg > 1:956605/1‑136 (MQ=255)
cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg < 1:725149/139‑4 (MQ=255)
tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg > 1:1456186/1‑129 (MQ=255)
tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg < 2:1456186/132‑4 (MQ=255)
cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgc < 2:659550/139‑1 (MQ=255)
cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg < 2:173028/119‑4 (MQ=255)
cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg > 1:173028/1‑116 (MQ=255)
gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTaaa < 1:357802/139‑1 (MQ=255)
tAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc < 2:956605/139‑1 (MQ=255)
aGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAt > 1:537735/1‑139 (MQ=255)
gTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg > 2:1218300/1‑134 (MQ=255)
gTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg < 1:1218300/134‑1 (MQ=255)
tGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta > 2:346811/1‑91 (MQ=255)
tGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta < 1:346811/92‑2 (MQ=255)
ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgcg < 1:1234835/106‑1 (MQ=255)
ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgcg > 2:1234835/1‑106 (MQ=255)
ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg > 2:1232618/1‑74 (MQ=255)
ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttacg < 1:1232618/77‑4 (MQ=255)
ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta > 2:340948/1‑74 (MQ=255)
ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta < 1:340948/75‑2 (MQ=255)
ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCg < 1:5753/105‑1 (MQ=255)
ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCg > 2:5753/1‑105 (MQ=255)
ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg > 2:404976/1‑139 (MQ=255)
atGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGa < 2:197564/139‑1 (MQ=255)
cTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta < 1:179392/63‑2 (MQ=255)
cTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta > 2:179392/1‑62 (MQ=255)
gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgc > 1:798528/1‑74 (MQ=255)
gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgc < 2:798528/74‑1 (MQ=255)
tttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta < 2:1136515/59‑2 (MQ=255)
tttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGttttta > 1:1136515/1‑58 (MQ=255)
ttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgcgc < 2:956379/74‑1 (MQ=255)
ttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAgcgc > 1:956379/1‑74 (MQ=255)
ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga > 1:37252/1‑116 (MQ=255)
ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga < 2:37252/116‑1 (MQ=255)
ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc < 1:1288840/139‑1 (MQ=255)
cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAg < 2:482532/101‑1 (MQ=255)
cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAg > 1:482532/1‑101 (MQ=255)
cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc > 1:1246731/1‑134 (MQ=255)
cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc < 2:1246731/134‑1 (MQ=255)
tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg > 2:666432/1‑124 (MQ=255)
tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg < 1:666432/124‑1 (MQ=255)
gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa < 2:537735/139‑1 (MQ=255)
gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa < 1:1415928/139‑1 (MQ=255)
gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa < 2:837816/139‑1 (MQ=255)
aCAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATAtt > 2:1519592/1‑139 (MQ=255)
aGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGa > 2:1449143/1‑139 (MQ=255)
ggTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGt < 2:179027/49‑1 (MQ=255)
ggTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGt > 1:179027/1‑49 (MQ=255)
ggTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGa > 1:482469/1‑139 (MQ=255)
|
ACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGA > NC_000913/1868882‑1869141
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
