Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
715 |
38.4 |
1606192 |
96.6% |
1551581 |
120.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,723,065 |
G→A |
A113A (GCG→GCA) |
pssA → |
phosphatidylserine synthase, CDP‑diacylglycerol‑serine O‑phosphatidyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,723,065 | 0 | G | A | 94.3%
| 97.3
/ ‑1.2
| 36 | A113A (GCG→GCA) | pssA | phosphatidylserine synthase, CDP‑diacylglycerol‑serine O‑phosphatidyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (16/17); total (18/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.07e-01 |
TTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGTT > NC_000913/2722937‑2723200
|
ttGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATc < 1:257639/139‑1 (MQ=255)
ttGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATc > 2:471927/1‑139 (MQ=255)
aTGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCggg > 2:272836/1‑139 (MQ=255)
gCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAg > 2:301068/1‑139 (MQ=255)
aaaGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGt > 2:712943/1‑139 (MQ=255)
aaaGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGt > 1:625957/1‑139 (MQ=255)
tGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGa < 1:594265/139‑1 (MQ=255)
aCTGGCATCGTGCACAACGTGGACGCGTTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGCCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCt < 1:725971/139‑1 (MQ=255)
aCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCt > 2:149854/1‑139 (MQ=255)
aCTGGCATCGTGCACAAC‑‑GGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTg < 2:112766/139‑1 (MQ=255)
tGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTg < 2:730093/139‑1 (MQ=255)
tGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTg < 1:272836/139‑1 (MQ=255)
caACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACt > 2:16816/1‑139 (MQ=255)
caACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACt > 2:404891/1‑139 (MQ=255)
caACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACt > 1:268412/1‑139 (MQ=255)
cGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTa < 1:301068/139‑1 (MQ=255)
aCGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTACAGGc > 2:68687/1‑139 (MQ=255)
aCGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGc < 1:712943/139‑1 (MQ=255)
gcgcTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTatcat < 2:46622/139‑1 (MQ=255)
tGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCCCAGGAAAATCCGGGCGTAGATGTTCCGGTTTa > 2:523203/1‑75 (MQ=255)
tGCGGCATCTAAAACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTa < 1:523203/75‑1 (MQ=255)
cGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATcgacga < 2:325785/139‑1 (MQ=255)
aTCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGc < 2:268412/139‑1 (MQ=255)
tCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGc > 1:606119/1‑109 (MQ=255)
tCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGc < 2:606119/109‑1 (MQ=255)
tCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCg < 1:149854/139‑1 (MQ=255)
tCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCg < 1:589434/139‑1 (MQ=255)
tAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGTTGTTTCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCCACGATAGCGTa < 1:26904/139‑1 (MQ=255)
tAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGt > 1:616641/1‑57 (MQ=255)
tAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGt < 2:616641/57‑1 (MQ=255)
aCGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAg > 2:624358/1‑139 (MQ=255)
aCGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAg > 2:116352/1‑139 (MQ=255)
aCGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAg > 2:751362/1‑139 (MQ=255)
gACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTg < 1:404891/139‑1 (MQ=255)
ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAAc > 2:623775/1‑139 (MQ=255)
ggCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGtt > 2:51493/1‑139 (MQ=255)
|
TTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGTT > NC_000913/2722937‑2723200
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
