Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	773	79.9	4112934	92.2%	3792125	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,723,065	G→A	A113A (GCG→GCA)	pssA →	phosphatidylserine synthase, CDP‑diacylglycerol‑serine O‑phosphatidyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,723,065	0	G	A	100.0%	229.4 / NA	71	A113A (GCG→GCA)	pssA	phosphatidylserine synthase, CDP‑diacylglycerol‑serine O‑phosphatidyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (31/40); total (31/40)

GTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAAC  >  NC_000913/2722936‑2723194

gTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAAt                                                                                                                          <  2:1725536/139‑1 (MQ=255)

          gCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAg                                                                                                                >  1:1914684/1‑139 (MQ=255)

              aaaGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCggg                                                                                                                     >  1:624302/1‑130 (MQ=255)

              aaaGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCggg                                                                                                                     <  2:624302/130‑1 (MQ=255)

              aaaGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGt                                                                                                            >  2:1623897/1‑139 (MQ=255)

              aaaGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGt                                                                                                            >  1:1347292/1‑139 (MQ=255)

               aaGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGtt                                                                                                           <  2:1914684/139‑1 (MQ=255)

                        tCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGAAGCATTGGCGCTGCGGCATCTCACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTAt                                                                                                  >  1:1828478/1‑139 (MQ=255)

                         ccGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATg                                                                                                 <  1:1736374/139‑1 (MQ=255)

                         ccGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATg                                                                                                 <  1:1749593/139‑1 (MQ=255)

                                   aTGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaat                                                                                       <  2:351507/139‑1 (MQ=255)

                                            tGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCg                                                                              >  1:206382/1‑139 (MQ=255)

                                                       tGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAg                                                                                                                <  1:670370/94‑1 (MQ=255)

                                                       tGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAg                                                                                                                >  2:670370/1‑94 (MQ=255)

                                                             cGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTc                                                             >  2:734424/1‑139 (MQ=255)

                                                               tGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTg                                                           <  2:1531018/139‑1 (MQ=255)

                                                               tGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTg                                                           <  1:1498173/139‑1 (MQ=255)

                                                                gCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGc                                                          <  2:1828478/139‑1 (MQ=255)

                                                                   caACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACt                                                       >  1:881724/1‑139 (MQ=255)

                                                                      cGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATa                                                                                  >  1:1782296/1‑109 (MQ=255)

                                                                      cGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATa                                                                                  <  2:1782296/109‑1 (MQ=255)

                                                                         ggACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAg                                                 >  1:1250699/1‑139 (MQ=255)

                                                                          gACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGtt                                                                                            >  2:996546/1‑95 (MQ=255)

                                                                          gACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGtt                                                                                            <  1:996546/95‑1 (MQ=255)

                                                                          gACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAgg                                                <  2:1250699/139‑1 (MQ=255)

                                                                           aCGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGc                                               >  1:431784/1‑139 (MQ=255)

                                                                                ttGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCca                                                                                         >  2:1457986/1‑92 (MQ=255)

                                                                                ttGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCca                                                                                         <  1:1457986/92‑1 (MQ=255)

                                                                                ttGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTg                                                           <  1:97121/122‑1 (MQ=255)

                                                                                ttGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTg                                                           >  2:97121/1‑122 (MQ=255)

                                                                                   gcgcTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTatcat                                       <  1:1916729/139‑1 (MQ=255)

                                                                                         cGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGtt                                                                                            >  2:1938694/1‑80 (MQ=255)

                                                                                         cGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGtt                                                                                            <  1:1938694/80‑1 (MQ=255)

                                                                                           gCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATa                               <  2:8416/139‑1 (MQ=255)

                                                                                             aTCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGc                             >  2:1747955/1‑139 (MQ=255)

                                                                                             aTCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGc                             <  1:1623897/139‑1 (MQ=255)

                                                                                             aTCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGc                             <  2:1595688/139‑1 (MQ=255)

                                                                                              tCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCg                            <  1:786133/139‑1 (MQ=255)

                                                                                              tCTAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCg                            <  2:1779873/139‑1 (MQ=255)

                                                                                                tAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTa                          <  1:1235495/139‑1 (MQ=255)

                                                                                                tAACACTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTa                          <  2:1347292/139‑1 (MQ=255)

                                                                                                   cacTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACtt                       >  2:562034/1‑139 (MQ=255)

                                                                                                    acTAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTATAGGCTTTATCATCGACGATAGCGTACttt                      <  1:408451/139‑1 (MQ=255)

                                                                                                      tAACGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTtat                    >  1:66418/1‑139 (MQ=255)

                                                                                                        aCGCTGACTGGTACTGCCGCATGGCACAGGAAAATc                                                                                                                         <  2:923296/36‑1 (MQ=255)

                                                                                                        aCGCTGACTGGTACTGCCGCATGGCACAGGAAAATc                                                                                                                         >  1:923296/1‑36 (MQ=38)

                                                                                                        aCGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAg                  >  1:957182/1‑139 (MQ=255)

                                                                                                        aCGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAg                  <  2:881724/139‑1 (MQ=255)

                                                                                                        aCGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAg                  <  1:734424/139‑1 (MQ=255)

                                                                                                        aCGCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAg                  <  1:562034/139‑1 (MQ=255)

                                                                                                          gCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGc                                                                        <  2:933568/83‑1 (MQ=255)

                                                                                                          gCTGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGc                                                                        >  1:933568/1‑83 (MQ=255)

                                                                                                            tGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGa                                                                           <  1:217401/78‑1 (MQ=255)

                                                                                                            tGACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGa                                                                           >  2:217401/1‑78 (MQ=255)

                                                                                                             gACTGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGAGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTg             <  1:888631/139‑1 (MQ=255)

                                                                                                                tGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCca                                                                                         <  2:283163/60‑1 (MQ=255)

                                                                                                                tGGTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCca                                                                                         >  1:283163/1‑60 (MQ=255)

                                                                                                                  gTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGc        >  1:1570625/1‑139 (MQ=255)

                                                                                                                  gTACTGCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGc        <  1:1747955/139‑1 (MQ=255)

                                                                                                                       gCCGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGaa   <  1:1211330/139‑1 (MQ=255)

                                                                                                                        ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCt                                              <  2:573916/95‑1 (MQ=255)

                                                                                                                        ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCt                                              >  1:573916/1‑95 (MQ=255)

                                                                                                                        ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATcga                                    <  2:215470/105‑1 (MQ=255)

                                                                                                                        ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATcga                                    >  1:215470/1‑105 (MQ=255)

                                                                                                                        ccGCATGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAAc  >  1:380132/1‑139 (MQ=255)

                                                                                                                            aTGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaatca                                                                                     <  1:1201727/52‑1 (MQ=255)

                                                                                                                            aTGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaatca                                                                                     >  2:1201727/1‑52 (MQ=255)

                                                                                                                             tGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTc                                                                                           >  1:166007/1‑45 (MQ=255)

                                                                                                                             tGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTc                                                                                           <  2:166007/45‑1 (MQ=255)

                                                                                                                             tGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGc                                                                        >  1:1215422/1‑64 (MQ=255)

                                                                                                                             tGGCACAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGc                                                                        <  2:1215422/64‑1 (MQ=255)

GTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAAGGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAAC  >  NC_000913/2722936‑2723194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A