Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	715	38.4	1606192	96.6%	1551581	120.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,936,931	T→C	F450L (TTC→CTC)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,936,931	0	T	C	100.0%	114.7 / NA	32	F450L (TTC→CTC)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/15); total (17/15)

CGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCA  >  NC_000913/2936796‑2937066

cgcgACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTcc                                                                                                                                       >  2:550819/1‑139 (MQ=255)

cgcgACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTcc                                                                                                                                       >  2:280181/1‑139 (MQ=255)

          aaGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑Agg                                                                                                                            >  2:52503/1‑139 (MQ=255)

                  ccGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTcc                                                                                                                    <  1:345259/139‑1 (MQ=255)

                                      ggAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTc                                                                                                >  1:290682/1‑139 (MQ=255)

                                                 aGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCaa                                                                                     <  2:503132/139‑1 (MQ=255)

                                                       agGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCt                                                                               >  1:392788/1‑139 (MQ=255)

                                                            gACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                                          <  1:282290/139‑1 (MQ=255)

                                                            gACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                                          <  2:80376/139‑1 (MQ=255)

                                                             aCGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAcc                                                                         >  2:450721/1‑139 (MQ=255)

                                                                   gCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACt                                                                   >  1:513954/1‑139 (MQ=255)

                                                                              aCCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCgg                                                        <  1:52503/139‑1 (MQ=255)

                                                                                  aaTGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAgg                                                    >  1:774290/1‑139 (MQ=255)

                                                                                       tGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGa                                               >  2:709195/1‑139 (MQ=255)

                                                                                         cccGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGc                                             >  2:440119/1‑139 (MQ=255)

                                                                                               gATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGaa                                       <  1:586589/139‑1 (MQ=255)

                                                                                               gATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGaa                                       <  2:790999/139‑1 (MQ=255)

                                                                                                 tCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAAtt                                     >  1:574736/1‑139 (MQ=255)

                                                                                                     cGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCg                                 <  2:513954/139‑1 (MQ=255)

                                                                                                     cGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCg                                 <  2:574736/139‑1 (MQ=255)

                                                                                                      gAATACTTCCGTGGCGGCCGTTACTCTTCCCGCCTCCTTACCGATCGGCGCCG‑CCCGTTGACCATAAGTGACGGCAACATCATGGAAGGCCTGTGACAGGTACTGCAAATCGCGGAAGGCTGGATCGTGTAATTGCCGa                                <  1:363438/139‑1 (MQ=255)

                                                                                                       aaTACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGaa                               <  1:125096/139‑1 (MQ=255)

                                                                                                         tACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAgg                             <  2:207217/139‑1 (MQ=255)

                                                                                                         tACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAgg                             <  1:450721/139‑1 (MQ=255)

                                                                                                           cTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGAt                           >  2:92381/1‑139 (MQ=255)

                                                                                                           cTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGAt                           >  2:695908/1‑139 (MQ=255)

                                                                                                           cTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGAt                           >  2:455558/1‑139 (MQ=255)

                                                                                                             tCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGAtgt                         >  2:370142/1‑139 (MQ=255)

                                                                                                              ccGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTccc                                                                                                                   >  2:56742/1‑48 (MQ=255)

                                                                                                              ccGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTccc                                                                                                                   <  1:56742/48‑1 (MQ=255)

                                                                                                                    cggcggTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGAc                  <  2:569357/139‑1 (MQ=255)

                                                                                                                                    cGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCa  >  2:181503/1‑139 (MQ=255)

CGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCA  >  NC_000913/2936796‑2937066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A