Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,936,931	T→C	F450L (TTC→CTC)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,936,931	0	T	C	100.0%	120.7 / NA	34	F450L (TTC→CTC)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/19); total (15/19)

GACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATG  >  NC_000913/2936799‑2937048

gACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTa                                                                                                                 >  1:20453/1‑139 (MQ=255)

             aCCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGt                                                                                                    <  1:166177/139‑1 (MQ=255)

             aCCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGt                                                                                                    <  1:319344/139‑1 (MQ=255)

              cccGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTc                                                                                                   >  2:12037/1‑139 (MQ=255)

                 gacgaTGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCg                                                                                                <  1:593051/139‑1 (MQ=255)

                    gaTGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTc                                                                                             >  1:524233/1‑139 (MQ=255)

                            cacaCTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGAc                                                                                     <  2:417382/139‑1 (MQ=255)

                             acacTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACt                                                                                    <  1:720476/139‑1 (MQ=255)

                               acTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGAc                                                                                     >  2:327589/1‑136 (MQ=255)

                               acTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGAc                                                                                     <  1:327589/136‑1 (MQ=255)

                               acTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCg                                                                                  >  2:720344/1‑139 (MQ=255)

                                  gggAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCgtgt                                                                               >  1:551218/1‑139 (MQ=255)

                                  gggAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCgtgt                                                                               >  2:290708/1‑139 (MQ=255)

                                   ggAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCGCGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTc                                                                              <  2:551218/139‑1 (MQ=255)

                                       aTCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAAca                                                                          <  2:242816/139‑1 (MQ=255)

                                        tctctcAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTc                                                                              <  1:338666/134‑1 (MQ=255)

                                        tctctcAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTc                                                                              >  2:338666/1‑134 (MQ=255)

                                             cAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACAtcatca                                                                    >  1:353194/1‑139 (MQ=255)

                                                              ttGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTa                                                   >  2:474287/1‑139 (MQ=255)

                                                                               aaTGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAgg                                  >  1:236358/1‑139 (MQ=255)

                                                                                    tGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGa                             >  2:671733/1‑139 (MQ=255)

                                                                                       ccGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCg                          <  1:290708/139‑1 (MQ=255)

                                                                                       ccGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCg                          <  1:256395/139‑1 (MQ=255)

                                                                                            gATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGaa                     <  1:396598/139‑1 (MQ=255)

                                                                                            gATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGaa                     <  2:353194/139‑1 (MQ=255)

                                                                                                      tACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAgg           <  2:524233/139‑1 (MQ=255)

                                                                                                        cTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                        <  2:168082/92‑1 (MQ=255)

                                                                                                        cTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                        >  1:168082/1‑92 (MQ=255)

                                                                                                          tCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAAACGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGAtgt       <  2:20453/139‑1 (MQ=255)

                                                                                                               ggcggcGGTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATg  <  2:236358/139‑1 (MQ=255)

                                                                                                                   gcggTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCg                          <  2:363411/111‑1 (MQ=255)

                                                                                                                   gcggTTACTCTTCCCGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCg                          >  1:363411/1‑111 (MQ=255)

                                                                                                                                ccGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCaa                                                                   >  1:178267/1‑57 (MQ=255)

                                                                                                                                ccGCCTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCaa                                                                   <  2:178267/57‑1 (MQ=255)

GACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATG  >  NC_000913/2936799‑2937048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A