Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	715	38.4	1606192	96.6%	1551581	120.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,447,891	G→A	1260 (TAG→TAA)	tamB →	translocation and assembly module for autotransporter export, inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,447,891	0	G	A	98.5%	214.5 / NA	67	1260 (TAG→TAA)	tamB	translocation and assembly module for autotransporter export, inner membrane subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (32/34); minor base T (1/0); total (33/34)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGC  >  NC_000913/4447782‑4448024

aCTCTATAGCAACACTCACGTTACGTTATAGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACGAGGCACTGGA‑TTGCTCTATCAGTGCGAGTTTTAACAATGCGAATATTTATCTACGGCAGTTTAc                                                                                                         >  2:535299/1‑139 (MQ=255)

    tataGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCca                                                                                                     >  2:308918/1‑139 (MQ=255)

    tataGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCca                                                                                                     >  1:27411/1‑139 (MQ=255)

    tataGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCca                                                                                                     >  1:412833/1‑139 (MQ=255)

    tataGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCATGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCca                                                                                                     >  1:214976/1‑139 (MQ=255)

      taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCcaca                                                                                                   >  2:425397/1‑139 (MQ=255)

      taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCcaca                                                                                                   >  2:700976/1‑139 (MQ=255)

        gCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCacaaa                                                                                                 <  2:490612/139‑1 (MQ=255)

          aaCACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCacaaaca                                                                                               <  1:700976/139‑1 (MQ=255)

          aaCACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCacaaaca                                                                                               <  1:434344/139‑1 (MQ=255)

            cacTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAg                                                                                             >  2:351087/1‑139 (MQ=255)

              cTCGCGTTACGTTATCGCCTGATGCCTAAGACATATCTGGCAGCCGTGTCTG‑‑GAGTAGGCCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAAGAATGCGAATATTTCTCTACGGACCTTTACGCCACAAACAAgtc                                                                                           >  1:238079/1‑137 (MQ=255)

              cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                           >  2:410411/1‑139 (MQ=255)

              cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                           >  1:590370/1‑139 (MQ=255)

                cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                         <  2:461084/139‑1 (MQ=255)

                 acgttacgttATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAAc                                                                                        >  2:539962/1‑139 (MQ=255)

                    ttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                                                     <  2:27411/139‑1 (MQ=255)

                       cgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCAc                                                                                  >  1:635629/1‑139 (MQ=255)

                            tCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                                             <  1:354855/139‑1 (MQ=255)

                            tCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                                             <  1:593026/139‑1 (MQ=255)

                                 tGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAc                                                                          <  1:70688/139‑1 (MQ=255)

                                      ccTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                                                   <  1:522650/139‑1 (MQ=255)

                                                 tCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGACACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTggg                                                        >  2:327489/1‑139 (MQ=255)

                                                   tGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCg                                                      <  2:241720/139‑1 (MQ=255)

                                                    ggAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGa                                                     >  1:679999/1‑139 (MQ=255)

                                                      aaGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAtt                                                   >  2:89056/1‑139 (MQ=255)

                                                         ccGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                                                >  2:542513/1‑139 (MQ=255)

                                                         ccGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                                                >  1:439789/1‑139 (MQ=255)

                                                         ccGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                                                >  2:704936/1‑139 (MQ=255)

                                                         ccGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACCGCAGTCTACGCCACAAACATGGCAACAGTCACTGTATGACCAATGCCCAGTTACTCGGCGATTGCa                                                >  1:50075/1‑139 (MQ=255)

                                                          cGTGTCTG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAg                                               <  2:797880/139‑1 (MQ=255)

                                                                tG‑‑GTGTAGACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGa                                         <  1:410411/139‑1 (MQ=255)

                                                                 g‑‑gTGTAGACCATGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGAt                                        <  2:519351/139‑1 (MQ=255)

                                                                         gACCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                                  <  2:256637/139‑1 (MQ=255)

                                                                          aCCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAc                                                                          >  1:715696/1‑98 (MQ=255)

                                                                          aCCAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAc                                                                          <  2:715696/98‑1 (MQ=255)

                                                                           ccAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACGAGGCAACAGGCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa                                <  1:79288/139‑1 (MQ=255)

                                                                           ccAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa                                <  1:285787/139‑1 (MQ=255)

                                                                           ccAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa                                <  2:50075/139‑1 (MQ=255)

                                                                           ccAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa                                <  1:542513/139‑1 (MQ=255)

                                                                            cAGGCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg                               <  2:429972/139‑1 (MQ=255)

                                                                              ggCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAAt                                                                                                                                   >  2:235430/1‑37 (MQ=255)

                                                                              ggCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAAt                                                                                                                                   <  1:235430/37‑1 (MQ=255)

                                                                              ggCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa                                >  2:199/1‑136 (MQ=255)

                                                                              ggCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa                                <  1:199/136‑1 (MQ=255)

                                                                               gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                         <  2:89436/78‑1 (MQ=255)

                                                                               gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                         >  1:89436/1‑78 (MQ=255)

                                                                               gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                         <  1:412077/78‑1 (MQ=255)

                                                                               gCACTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                         >  2:412077/1‑78 (MQ=255)

                                                                                 aCTGGA‑TTGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATTACTACCAGTTGta                          <  1:539962/139‑1 (MQ=255)

                                                                                     ggattGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                         <  1:225448/70‑1 (MQ=255)

                                                                                     ggattGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                         >  2:225448/4‑73 (MQ=255)

                                                                                         tGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTg                   <  2:777661/139‑1 (MQ=255)

                                                                                         tGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTg                   <  1:670471/139‑1 (MQ=255)

                                                                                           ctctATCAGTTCGAGTTTTATCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTggg                 >  1:469785/1‑139 (MQ=255)

                                                                                              tATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCa              <  1:351087/139‑1 (MQ=255)

                                                                                                tCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCcaca                                                                                                   <  2:538502/52‑1 (MQ=255)

                                                                                                tCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCcaca                                                                                                   >  1:538502/1‑52 (MQ=255)

                                                                                                     tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACt                                    <  1:531943/110‑1 (MQ=255)

                                                                                                     tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACt                                    >  2:531943/1‑110 (MQ=255)

                                                                                                     tCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCa       >  2:587374/1‑139 (MQ=255)

                                                                                                        aGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGta                          <  1:286460/117‑1 (MQ=255)

                                                                                                        aGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGta                          >  2:286460/1‑117 (MQ=255)

                                                                                                         gTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGgcg   <  1:327489/139‑1 (MQ=255)

                                                                                                          ttttAACGATGCGAATATTTGTCTACGGCAGTTTACGCCATAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGgcgc  >  2:488540/1‑139 (MQ=255)

                                                                                                          ttttAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                         <  2:237088/52‑1 (MQ=255)

                                                                                                          ttttAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                         >  1:237088/1‑52 (MQ=255)

ACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTG‑‑GTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGC  >  NC_000913/4447782‑4448024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A