Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,456,515	A→G	Q244R (CAG→CGG)	mpl →	UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,456,515	0	A	G	100.0%	215.3 / NA	60	Q244R (CAG→CGG)	mpl	UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (30/30); total (30/30)

TTCCACCATCTGGTGCGTATCGTTCCGGGGCAGGGCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCAGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGC  >  NC_000913/4456394‑4456636

ttCCACCATCTGGTGCGTATCGTTCCGGGGCAGGGCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGa                                                                                                          >  2:1156940/1‑139 (MQ=255)

 tCCACCATCTGGTGCGTATCGTTCCGGGGCAGGGCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGaa                                                                                                         >  1:392977/1‑139 (MQ=255)

            gTGCGTATCGTTCCGGGGCAGGGCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGacca                                                                                              >  1:822296/1‑139 (MQ=255)

                           gggCAGGGCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTg                                                                                                  <  2:1400369/120‑1 (MQ=255)

                           gggCAGGGCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTg                                                                                                  >  1:1400369/1‑120 (MQ=255)

                                gggCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCgggg                                                                                                                        >  2:1386329/1‑93 (MQ=255)

                                gggCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCgggg                                                                                                                        <  1:1386329/93‑1 (MQ=255)

                                 ggCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAg                                                                                                     >  2:76931/1‑111 (MQ=255)

                                 ggCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAg                                                                                                     <  1:76931/111‑1 (MQ=255)

                                                ccGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATg                                                             <  2:865474/136‑1 (MQ=255)

                                                ccGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATg                                                             >  1:865474/1‑136 (MQ=255)

                                                ccGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCg                                                          >  1:1243370/1‑139 (MQ=255)

                                                ccGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCg                                                          >  1:500009/1‑139 (MQ=255)

                                                  ggAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGaa                                                        >  1:1518845/1‑139 (MQ=255)

                                                          aCATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTggg                                                <  1:286460/139‑1 (MQ=255)

                                                              cAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCg                                              <  1:1283081/137‑1 (MQ=255)

                                                              cAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCg                                              >  2:1283081/1‑137 (MQ=255)

                                                                      aaCAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCTCCGATTCTTCCGAATTGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCTAAGTGAAATg                                    <  1:1570238/139‑1 (MQ=255)

                                                                         aGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTc                                 <  2:392977/139‑1 (MQ=255)

                                                                          gACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCg                                <  1:647971/139‑1 (MQ=255)

                                                                           aCCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGaa                                       <  2:1550493/131‑1 (MQ=255)

                                                                           aCCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGaa                                       >  1:1550493/1‑131 (MQ=255)

                                                                           aCCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGc                               >  2:119530/1‑139 (MQ=255)

                                                                           aCCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGACGGCGAAAAAGTGGGCGAAGTGAAATGGTCGc                               >  1:1362299/1‑139 (MQ=255)

                                                                            ccATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAg                                                                                                     <  1:537453/68‑1 (MQ=255)

                                                                            ccATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAg                                                                                                     >  2:537453/1‑68 (MQ=255)

                                                                              aTGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTgg                            <  2:1362299/139‑1 (MQ=255)

                                                                               tGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGt                           >  1:1142237/1‑139 (MQ=255)

                                                                                  cGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAgg                        <  1:119530/139‑1 (MQ=255)

                                                                                    aTGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGa                                                                                                          <  1:1036197/55‑1 (MQ=255)

                                                                                    aTGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGa                                                                                                          >  2:1036197/1‑55 (MQ=255)

                                                                                     tGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGacc                                                                                               >  2:181615/1‑65 (MQ=255)

                                                                                     tGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGacc                                                                                               <  1:181615/65‑1 (MQ=255)

                                                                                     tGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGa                     <  2:1142237/139‑1 (MQ=255)

                                                                                       ggCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGacc                                                                                               >  1:1383320/1‑63 (MQ=255)

                                                                                       ggCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGacc                                                                                               <  2:1383320/63‑1 (MQ=255)

                                                                                       ggCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACGACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   <  1:1156940/139‑1 (MQ=255)

                                                                                       ggCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   <  2:439391/139‑1 (MQ=255)

                                                                                       ggCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   <  1:1230381/139‑1 (MQ=255)

                                                                                       ggCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   >  1:1236646/1‑139 (MQ=255)

                                                                                       ggCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   <  2:1518845/139‑1 (MQ=255)

                                                                                           gctgGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   >  2:1248776/1‑135 (MQ=255)

                                                                                           gctgGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   <  1:1248776/135‑1 (MQ=255)

                                                                                            ctgGAGCGAGCAGGGGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   <  1:763830/134‑1 (MQ=255)

                                                                                            ctgGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTg                                                                    >  1:253479/1‑85 (MQ=255)

                                                                                            ctgGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTg                                                                    <  2:253479/85‑1 (MQ=255)

                                                                                            ctgGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAgg                        >  2:1533842/1‑129 (MQ=255)

                                                                                            ctgGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAgg                        <  1:1533842/129‑1 (MQ=255)

                                                                                            ctgGAGCGAGCAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAAc                   >  2:763830/1‑134 (MQ=255)

                                                                                                    agcAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGa                     <  1:622741/124‑1 (MQ=255)

                                                                                                    agcAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGa                     >  2:622741/1‑124 (MQ=255)

                                                                                                    agcAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAatat            <  1:1406603/133‑1 (MQ=255)

                                                                                                    agcAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAatat            >  2:1406603/1‑133 (MQ=255)

                                                                                                    agcAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACaa      >  2:354185/1‑139 (MQ=255)

                                                                                                    agcAGGAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTACGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACaa      >  2:850305/1‑139 (MQ=255)

                                                                                                        ggAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGc  <  2:500009/139‑1 (MQ=255)

                                                                                                         gAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCg                                >  2:1273977/1‑108 (MQ=255)

                                                                                                         gAGCTGGTGGGTGAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCg                                <  1:1273977/108‑1 (MQ=255)

                                                                                                                 gggtgAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTggg                                                <  2:384949/84‑1 (MQ=255)

                                                                                                                 gggtgAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTggg                                                >  1:384949/1‑84 (MQ=255)

                                                                                                                   gtgAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAg                                                                         >  2:1459216/1‑57 (MQ=255)

                                                                                                                   gtgAGCGGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAg                                                                         <  1:1459216/57‑1 (MQ=255)

TTCCACCATCTGGTGCGTATCGTTCCGGGGCAGGGCCGTATTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCAGGGTCACTGGCAGGCGAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGCTGGATGGCGAAAAAGTGGGCGAAGTGAAATGGTCGCTGGTAGGCGAACATAATATGCACAATGGC  >  NC_000913/4456394‑4456636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A