Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	758	59.3	2864096	93.9%	2689386	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,460,932	A→G	F577L (TTC→CTC)	nrdD ←	anaerobic ribonucleoside‑triphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,460,932	0	A	G	100.0%	166.8 / NA	48	F577L (TTC→CTC)	nrdD	anaerobic ribonucleoside‑triphosphate reductase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/26); total (22/26)

CAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGTTTCTTCT  >  NC_000913/4460798‑4461065

cAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTg                                                                                                                                   <  1:930585/139‑1 (MQ=255)

   cgcCTTCAGGTTGTGCTGAATGTTTGGATACTAGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTg                                                                                                                                >  2:848959/1‑139 (MQ=255)

       ttCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGgtgt                                                                                                                            >  2:788742/1‑139 (MQ=255)

                             gATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGcc                                                                                                      <  2:1373491/139‑1 (MQ=255)

                                   cGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGcac                                                                                                <  1:354203/139‑1 (MQ=255)

                                    gCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGcacc                                                                                               >  2:1038858/1‑139 (MQ=255)

                                      cGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGAGGAGACCGTTGGTGTAGTAACCTTTATCGGTTAACGCCGGcaccac                                                                                             >  2:1190598/1‑139 (MQ=255)

                                       gTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACg                                                                                            <  2:485121/139‑1 (MQ=255)

                                            aaaTGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCaaa                                                                                       <  2:1391871/139‑1 (MQ=255)

                                            aaaTGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCaaa                                                                                       <  1:635812/139‑1 (MQ=255)

                                                gAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTca                                                                                   >  1:381115/1‑139 (MQ=255)

                                                 aaaCCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATTGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTcag                                                                                  <  1:848959/139‑1 (MQ=255)

                                                 aaaCCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTcag                                                                                  <  1:212275/139‑1 (MQ=255)

                                                          ttCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCga                                                                         <  1:1038858/139‑1 (MQ=255)

                                                            cGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTg                                                                                                                                >  2:439085/1‑82 (MQ=255)

                                                            cGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCgaga                                                                       <  2:407593/139‑1 (MQ=255)

                                                            cGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACAACGAGGTGGAGACTGTTg                                                                                                                                <  1:439085/82‑1 (MQ=255)

                                                               cAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTgtag                                                                                                                          <  2:135241/85‑1 (MQ=255)

                                                               cAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTgtag                                                                                                                          >  1:135241/1‑85 (MQ=255)

                                                                     cggGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAg                                                              <  2:381115/139‑1 (MQ=255)

                                                                       ggTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGcc                                                                                          >  2:118026/1‑109 (MQ=255)

                                                                       ggTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGcc                                                                                          <  1:118026/109‑1 (MQ=255)

                                                                             gcgcTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTTGAGACTGTTGGTGTACTATCCTTTATCGGTCACGCCCGCCACCACGCCAAAGTCAGCAGTATCGAGACGGCAGAAGCGATCGca                                                      <  2:1016062/139‑1 (MQ=255)

                                                                             gcgcTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGca                                                      <  1:57797/139‑1 (MQ=255)

                                                                             gcgcTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGca                                                      <  1:1190598/139‑1 (MQ=255)

                                                                                  tCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGTAGTATCGAGACGGCAGAAGCGATCGCACAGGt                                                 >  2:10415/1‑139 (MQ=255)

                                                                                                  cGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCa                                                                  >  1:501863/1‑106 (MQ=255)

                                                                                                  cGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCa                                                                  <  2:501863/106‑1 (MQ=255)

                                                                                                      cGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCgaga                                                                       >  2:23320/1‑97 (MQ=255)

                                                                                                      cGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCgaga                                                                       <  1:23320/97‑1 (MQ=255)

                                                                                                      cGGGTTCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTag                             >  1:746908/1‑139 (MQ=255)

                                                                                                          ttCACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGTAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGAc                         >  2:1000786/1‑139 (MQ=255)

                                                                                                            cACCTTCTTCTCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTg                       >  1:810405/1‑139 (MQ=255)

                                                                                                                   ttctCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCg                                        >  1:380607/1‑115 (MQ=255)

                                                                                                                   ttctCCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCg                                        <  2:380607/115‑1 (MQ=255)

                                                                                                                    tctcCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGACAGAAGCGATCGcaca                                                    <  1:105495/102‑1 (MQ=255)

                                                                                                                    tctcCACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGACAGAAGCGATCGcaca                                                    >  2:105495/1‑102 (MQ=255)

                                                                                                                       ccACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCaaa                                                                                       <  1:524991/64‑1 (MQ=255)

                                                                                                                       ccACATCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCaaa                                                                                       >  2:524991/1‑64 (MQ=255)

                                                                                                                         acaTCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTc                                                                                                           <  1:1168975/42‑1 (MQ=255)

                                                                                                                         acaTCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTc                                                                                                           >  2:1168975/1‑42 (MQ=255)

                                                                                                                          caTCGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGt         <  2:746908/139‑1 (MQ=255)

                                                                                                                             cGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGTttc      >  2:107451/1‑139 (MQ=255)

                                                                                                                             cGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGTttc      <  1:107451/139‑1 (MQ=255)

                                                                                                                             cGAGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAACAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATATCCCGTttc      >  1:849691/1‑139 (MQ=255)

                                                                                                                               aGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGTttctt    >  1:96672/1‑139 (MQ=255)

                                                                                                                               aGGTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGTttctt    >  2:327592/1‑139 (MQ=255)

                                                                                                                                ggTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGTttcttc   >  1:11275/1‑139 (MQ=255)

                                                                                                                                 gTGGAGACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGTTtcttct  <  2:11275/139‑1 (MQ=255)

CAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTTCACTCGGCGTGCTGTAGAGACTGAAACCATAACCCGTTTCTTCT  >  NC_000913/4460798‑4461065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A