Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F1 I2 R1 777 59.2 2955938 92.5% 2734242 106.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 395,245 G→A A348V (GCG→GTG)  ampH ← D‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase, penicillin‑binding protein, weak beta‑lactamase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913395,2450GA100.0% 197.2 / NA 62A348V (GCG→GTG) ampHD‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase, penicillin‑binding protein, weak beta‑lactamase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/35);  total (27/35)

GTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACC  >  NC_000913/395110‑395352
                                                                                                                                       |                                                                                                           
gTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACacgc                                                                                                          <  1:300822/139‑1 (MQ=255)
gTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACacgc                                                                                                          <  1:680571/139‑1 (MQ=255)
  ttGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCa                                                                                                        >  2:525448/1‑139 (MQ=255)
         cAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATAttct                                                                                                 <  2:1233624/139‑1 (MQ=255)
                   aTCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat                                                                                       <  2:1457601/139‑1 (MQ=255)
                   aTCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat                                                                                       <  1:1280059/139‑1 (MQ=255)
                      cggACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatcat                                                                                    <  1:1238345/138‑1 (MQ=255)
                      aGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatcat                                                                                    <  2:114517/139‑1 (MQ=255)
                        gACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGc                                                                                            >  2:137543/1‑129 (MQ=255)
                        gACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGc                                                                                            <  1:137543/129‑1 (MQ=255)
                        gACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCg                                                                                  >  2:164714/1‑139 (MQ=255)
                        gACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCg                                                                                  >  1:213400/1‑139 (MQ=255)
                          cgcgGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat                                                                                       <  2:458366/132‑1 (MQ=255)
                          cgcgGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat                                                                                       >  1:458366/1‑132 (MQ=255)
                          cgcgGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGcc                                                                                >  1:1330469/1‑139 (MQ=255)
                              gggATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCatat                                                                            <  1:164714/139‑1 (MQ=255)
                                     ccAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAAt                                                                     <  1:525448/139‑1 (MQ=255)
                                             ttATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGc                                                             >  1:1471913/1‑139 (MQ=255)
                                               aTTCCCGCTTAGCTCGGTCACCAGG‑CATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGccac                                                          >  1:1125741/1‑139 (MQ=255)
                                                ttCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGccac                                                          <  2:1330469/139‑1 (MQ=255)
                                                   ccGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGGCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCcaccac                                                       <  1:33774/139‑1 (MQ=255)
                                                          gCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCat                                                                              <  2:849506/109‑1 (MQ=255)
                                                          gCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCCCGTCAAGGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCat                                                                              >  1:849506/1‑109 (MQ=255)
                                                               gTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGcca                                                           >  2:1379354/1‑123 (MQ=255)
                                                               gTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGcca                                                           <  1:1379354/123‑1 (MQ=255)
                                                                          aTTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATcccc                                >  2:462322/1‑139 (MQ=255)
                                                                            tGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata                                                                             <  1:1094271/92‑1 (MQ=255)
                                                                            tGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata                                                                             >  2:1094271/1‑92 (MQ=255)
                                                                              aTGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGATCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGgac                            <  1:179065/139‑1 (MQ=255)
                                                                              aTGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGgac                            >  2:6422/1‑139 (MQ=255)
                                                                                 ccATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatcat                                                                                    >  1:804269/1‑80 (MQ=255)
                                                                                 ccATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatcat                                                                                    <  2:804269/80‑1 (MQ=255)
                                                                                  cATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatcatc                                                                                   >  1:1339335/1‑80 (MQ=255)
                                                                                  cATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatcatc                                                                                   <  2:1339335/80‑1 (MQ=255)
                                                                                    tCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat                                                                                       >  2:319845/1‑74 (MQ=255)
                                                                                    tCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat                                                                                       <  1:319845/74‑1 (MQ=255)
                                                                                     cGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCa                                                                               <  1:113976/81‑1 (MQ=255)
                                                                                     cGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCa                                                                               >  2:113976/1‑81 (MQ=255)
                                                                                           tatTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata                                                                             <  1:849374/77‑1 (MQ=255)
                                                                                           tatTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata                                                                             >  2:849374/1‑77 (MQ=255)
                                                                                              ttttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCCTATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGc            <  1:859243/139‑1 (MQ=255)
                                                                                              ttttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGc            <  2:1150393/139‑1 (MQ=255)
                                                                                              ttttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGc            <  1:6422/139‑1 (MQ=255)
                                                                                               tttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGcc           >  2:1386417/1‑139 (MQ=255)
                                                                                                ttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata                                                                             >  2:647597/1‑72 (MQ=255)
                                                                                                ttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCata                                                                             <  1:647597/72‑1 (MQ=255)
                                                                                                ttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGc            <  2:515734/137‑1 (MQ=255)
                                                                                                ttAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGc            >  1:515734/1‑137 (MQ=255)
                                                                                                  aaaGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatca                                                                                     >  2:1308581/1‑62 (MQ=255)
                                                                                                  aaaGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGatca                                                                                     <  1:1308581/62‑1 (MQ=255)
                                                                                                  aaaGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGaa                                                                  <  1:1103022/81‑1 (MQ=255)
                                                                                                  aaaGCGCGTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGaa                                                                  >  2:1103022/1‑81 (MQ=255)
                                                                                                      cgcgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCAtatata    >  1:1154285/1‑139 (MQ=255)
                                                                                                       gcgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATAc   <  2:1125741/139‑1 (MQ=255)
                                                                                                        cgTCAACGGCGAACGAGTGACCACCACCAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGGCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATAcc  <  2:1154285/139‑1 (MQ=255)
                                                                                                        cgTCAACGGCGAACGAGTGACCACCACAAACacgc                                                                                                          <  1:680068/35‑1 (MQ=38)
                                                                                                        cgTCAACGGCGAACGAGTGACCACCACAAACacgc                                                                                                          >  2:680068/1‑35 (MQ=255)
                                                                                                        cgTCAACGGCGAACGAGTGACCACCACAAACacgc                                                                                                          <  1:1231042/35‑1 (MQ=255)
                                                                                                        cgTCAACGGCGAACGAGTGACCACCACAAACacgc                                                                                                          >  2:1231042/1‑35 (MQ=255)
                                                                                                        cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat                                                                                       <  2:300686/54‑1 (MQ=255)
                                                                                                        cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGat                                                                                       >  1:300686/1‑54 (MQ=255)
                                                                                                        cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATAcc  <  2:213400/139‑1 (MQ=255)
                                                                                                        cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATAcc  <  1:529567/139‑1 (MQ=255)
                                                                                                        cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATAcc  >  2:1475790/1‑139 (MQ=255)
                                                                                                        cgTCAACGGCGAACGAGTGACCACCACAAACACGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATAcc  <  1:820309/139‑1 (MQ=255)
                                                                                                                           accaccACAAACACGCCAATATTCTTCTGCGGGatcat                                                                                    <  1:896884/38‑1 (MQ=255)
                                                                                                                             caccacAAACACGCCAATATTCTTCTGCGGGATCATCGCCata                                                                             <  1:776218/43‑1 (MQ=255)
                                                                                                                             caccacAAACACGCCAATATTCTTCTGCGGGATCATCGCCata                                                                             >  2:776218/1‑43 (MQ=255)
                                                                                                                                       |                                                                                                           
GTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGACCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACC  >  NC_000913/395110‑395352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A