Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	677,889	G→A	A159T (GCC→ACC)	djlB →	putative HscC co‑chaperone, uncharacterized J domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	677,889	0	G	A	100.0%	90.9 / NA	29	A159T (GCC→ACC)	djlB	putative HscC co‑chaperone, uncharacterized J domain‑containing protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/14); total (15/14)

GTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATT  >  NC_000913/677753‑678024

gTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATAcc                                                                                                                                       >  1:338581/1‑139 (MQ=255)

gTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATAcc                                                                                                                                       >  1:525627/1‑139 (MQ=255)

      gtgCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCg                                                                                                                                 <  2:1188924/139‑1 (MQ=255)

                     gAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGttttt                                                                                                                  <  2:1190438/139‑1 (MQ=255)

                           tGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCa                                                                                                                                      <  1:1006906/113‑1 (MQ=255)

                           tGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCa                                                                                                                                      >  2:1006906/1‑113 (MQ=255)

                               cATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGa                                                                                                        <  2:525627/139‑1 (MQ=255)

                                aTTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGAc                                                                                                       <  1:665143/139‑1 (MQ=255)

                                     tCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGa                                                                                                                          >  2:373062/1‑115 (MQ=255)

                                     tCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGa                                                                                                                          <  1:373062/115‑1 (MQ=255)

                                            aGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGa                                                                                                        >  1:119507/1‑126 (MQ=255)

                                            aGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGa                                                                                                        <  2:119507/126‑1 (MQ=255)

                                            aGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCt                                                                                           >  1:2427/1‑139 (MQ=255)

                                            aGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCt                                                                                           >  2:1249797/1‑139 (MQ=255)

                                                               aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCa                                                                                                                                      <  1:822431/77‑1 (MQ=255)

                                                               aGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCa                                                                                                                                      >  2:822431/1‑77 (MQ=255)

                                                                           gtggtTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTg                                                                                   <  2:1170303/116‑1 (MQ=255)

                                                                           gtggtTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTg                                                                                   >  1:1170303/1‑116 (MQ=255)

                                                                           gtggtTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGAt                                                            >  1:1362084/1‑139 (MQ=255)

                                                                               ttACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGAttttt                                                        >  1:487893/1‑139 (MQ=255)

                                                                                             tACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCt                                          <  1:49911/139‑1 (MQ=255)

                                                                                                 ggggAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGa                                      >  2:812162/1‑139 (MQ=255)

                                                                                                  gggAAATAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGAt                                     >  1:82591/1‑139 (MQ=255)

                                                                                                        tAGACGAAGAAGAACTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTc                               <  2:2427/139‑1 (MQ=255)

                                                                                                               agaagaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTc                                         >  1:259210/1‑122 (MQ=255)

                                                                                                               agaagaaCTGGAATCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTc                                         <  2:259210/122‑1 (MQ=255)

                                                                                                                           aTCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGa                                                                      >  2:901122/1‑81 (MQ=255)

                                                                                                                           aTCCTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGa                                                                      <  1:901122/81‑1 (MQ=255)

                                                                                                                              cTTTTTATATACCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACa         <  1:1249797/139‑1 (MQ=255)

                                                                                                                                     tataCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTAtt  <  2:487893/139‑1 (MQ=255)

GTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATT  >  NC_000913/677753‑678024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A