Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I1 R1 757 76.1 3598070 93.4% 3360597 108.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 678,964 T→C N148S (AAT→AGT)  ybeT ← Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913678,9640TC100.0% 243.4 / NA 67N148S (AAT→AGT) ybeTSel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/33);  total (34/33)

CTGTTCGGGTGACAGTCGACGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATCCACCCCAGTTTACT  >  NC_000913/678831‑679097
                                                                                                                                     |                                                                                                                                     
cTGTTCGGGTGACAGTCGACGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTg                                                                                                                                  >  1:958384/1‑139 (MQ=255)
   ttCGGGTGACAGTCGACGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAg                                                                                                                               >  2:1739266/1‑139 (MQ=255)
     cGGGTGACAGTCGACGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGAt                                                                                                                             >  1:680991/1‑139 (MQ=255)
       ggTGACAGTCGACGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTg                                                                                                                                  <  2:1486056/132‑1 (MQ=255)
       ggTGACAGTCGACGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTg                                                                                                                                  >  1:1486056/1‑132 (MQ=255)
                  aCGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGta                                                                                                                <  1:1537964/139‑1 (MQ=255)
                    gTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGtata                                                                                                              <  2:1794790/139‑1 (MQ=255)
                    gTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGtata                                                                                                              <  1:1739266/139‑1 (MQ=255)
                           tgCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATtgagca                                                                                                       >  2:1722724/1‑139 (MQ=255)
                             cGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATtgagcatg                                                                                                     >  2:825588/1‑139 (MQ=255)
                                 aaGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTTACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATtgagcatgagca                                                                                                 <  2:958384/139‑1 (MQ=255)
                                  aGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCt                                                                                                                      <  2:87454/117‑1 (MQ=255)
                                  aGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCt                                                                                                                      >  1:87454/1‑117 (MQ=255)
                                        gCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTaaa                                                                                                                   >  2:509566/1‑114 (MQ=255)
                                        gCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTaaa                                                                                                                   <  1:509566/114‑1 (MQ=255)
                                         cAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTg                                                                                         <  1:1101666/139‑1 (MQ=255)
                                                    tCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCtt                                                                              >  2:538697/1‑139 (MQ=255)
                                                      cGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTACTTcc                                                                            <  2:680991/139‑1 (MQ=255)
                                                       gCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCt                                                                           >  2:390994/1‑139 (MQ=255)
                                                       gCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCt                                                                           >  1:1432221/1‑139 (MQ=255)
                                                              tGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCa                                                                    <  2:1432221/139‑1 (MQ=255)
                                                                   tgttgtTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGaa                                                               <  1:1722724/139‑1 (MQ=255)
                                                                        tttGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGATCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAAt                                                              >  1:1700845/1‑135 (MQ=255)
                                                                        tttGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAAt                                                              <  2:1700845/135‑1 (MQ=255)
                                                                        tttGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCt                                                          >  1:1590225/1‑139 (MQ=255)
                                                                        tttGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCt                                                          >  2:1172891/1‑139 (MQ=255)
                                                                          tGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAgctg                                                                                 <  2:244032/114‑1 (MQ=255)
                                                                          tGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAgctg                                                                                 >  1:244032/1‑114 (MQ=255)
                                                                             cGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTg                                                                                                                                  >  2:793204/1‑62 (MQ=255)
                                                                             cGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTg                                                                                                                                  <  1:793204/62‑1 (MQ=255)
                                                                             cGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAAc                                                                                                                        >  2:891722/1‑72 (MQ=255)
                                                                             cGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAAc                                                                                                                        <  1:891722/72‑1 (MQ=255)
                                                                               gTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATtgagcatgagca                                                                                                 <  1:457681/93‑1 (MQ=255)
                                                                               gTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATtgagcatgagca                                                                                                 >  2:457681/1‑93 (MQ=255)
                                                                               gTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAgctgc                                                                                >  1:758027/1‑110 (MQ=255)
                                                                               gTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAgctgc                                                                                <  2:758027/110‑1 (MQ=255)
                                                                               gTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACggg                                                   >  2:225790/1‑139 (MQ=255)
                                                                                          ccATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTg                                        <  1:390994/139‑1 (MQ=255)
                                                                                            aTTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTa                                                       <  2:453259/122‑1 (MQ=255)
                                                                                            aTTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTa                                                       >  1:453259/1‑122 (MQ=255)
                                                                                              ttttGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTaaa                                                                                                                   >  2:1660173/1‑60 (MQ=255)
                                                                                              ttttGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTaaa                                                                                                                   <  1:1660173/60‑1 (MQ=255)
                                                                                              ttttGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGtt                                                                                       <  2:487894/88‑1 (MQ=255)
                                                                                              ttttGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGtt                                                                                       >  1:487894/1‑88 (MQ=255)
                                                                                                 tGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCa                                 <  2:1590225/139‑1 (MQ=255)
                                                                                                 tGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCa                                 <  1:825588/139‑1 (MQ=255)
                                                                                                  gagaTTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAg                                <  1:538697/139‑1 (MQ=255)
                                                                                                                  gaCATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGTATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGAt                 <  1:1063708/137‑1 (MQ=255)
                                                                                                                  aaCATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAgctgc                                                                                >  2:1105436/1‑75 (MQ=255)
                                                                                                                  aaCATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAgctgc                                                                                <  1:1105436/75‑1 (MQ=255)
                                                                                                                  aaCATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGgtgt                                                >  2:92551/1‑107 (MQ=255)
                                                                                                                  aaCATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGgtgt                                                <  1:92551/107‑1 (MQ=255)
                                                                                                                  aaCATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGAt                 >  2:1063708/1‑138 (MQ=255)
                                                                                                                  aaCATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATc                >  2:467849/1‑139 (MQ=255)
                                                                                                                      ttAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATCCAcc            <  1:225790/139‑1 (MQ=255)
                                                                                                                         aTACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATAccc                                                                                            <  2:1541302/56‑1 (MQ=255)
                                                                                                                         aTACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATAccc                                                                                            >  1:1541302/1‑56 (MQ=255)
                                                                                                                          tACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATCCACCCCAg        <  1:467849/139‑1 (MQ=255)
                                                                                                                              tGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAgctg                                                                                 >  2:1411769/1‑62 (MQ=255)
                                                                                                                              tGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAgctg                                                                                 <  1:1411769/62‑1 (MQ=255)
                                                                                                                               gAGCCACTTCTGTAGATCAAACCTAAAGTATATtgagcatgagca                                                                                                 <  1:1028288/45‑1 (MQ=255)
                                                                                                                               gAGCCACTTCTGTAGATCAAACCTAAAGTATATtgagcatgagca                                                                                                 >  2:1028288/1‑45 (MQ=255)
                                                                                                                               gAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTa                                                       <  1:1266595/87‑1 (MQ=255)
                                                                                                                               gAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTa                                                       >  2:1266595/1‑87 (MQ=255)
                                                                                                                               gAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATCCACCCCAGTTTAc   >  1:202580/1‑139 (MQ=255)
                                                                                                                               gAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATCCACCCCAGTTTAc   >  2:51998/1‑139 (MQ=255)
                                                                                                                                aGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATCCACCCCAGTTTACt  >  2:1671119/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                     
CTGTTCGGGTGACAGTCGACGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATCCACCCCAGTTTACT  >  NC_000913/678831‑679097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A