Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I2 R1 760 67.6 3323148 92.7% 3080558 109.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 678,964 T→C N148S (AAT→AGT)  ybeT ← Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913678,9640TC100.0% 249.1 / NA 69N148S (AAT→AGT) ybeTSel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/36);  total (33/36)

CATCTGTTCGGGTGACAGTCGACGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATCCACCCCAGTTTA  >  NC_000913/678828‑679095
                                                                                                                                        |                                                                                                                                   
cATCTGTTCGGGTGACAGTCGACGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCActt                                                                                                                                   >  2:105701/1‑139 (MQ=255)
  tCTGTTCGGGTGACAGTCGACGTTAATGTGCGGGAAGCCCGGCAAGTAATCTATCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCt                                                                                                                                 >  2:959092/1‑139 (MQ=255)
      ttCGGGTGACAGTCGACGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAg                                                                                                                             >  2:1656572/1‑139 (MQ=255)
        cGGGTGACAGTCGACGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGAt                                                                                                                           >  1:1596436/1‑139 (MQ=255)
         gggTGACAGTCGACGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATc                                                                                                                          <  1:1506314/139‑1 (MQ=255)
             gACAGTCGACGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAAc                                                                                                                      <  2:818897/139‑1 (MQ=255)
                aGTCGACGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTa                                                                                                                   >  1:256806/1‑139 (MQ=255)
                     aCGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGta                                                                                                              >  2:731076/1‑139 (MQ=255)
                     aCGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGta                                                                                                              <  1:1536650/139‑1 (MQ=255)
                       gTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGtata                                                                                                            <  2:65738/139‑1 (MQ=255)
                            tgtgCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATtgag                                                                                                       <  2:693035/139‑1 (MQ=255)
                             gtgCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATtgagc                                                                                                      <  2:256806/139‑1 (MQ=255)
                              tgCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATtgagca                                                                                                     <  1:898826/139‑1 (MQ=255)
                                    aaGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATtgagcatgagca                                                                                               <  2:1596436/139‑1 (MQ=255)
                                      gCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATtg                                                                                                         <  2:267648/127‑1 (MQ=255)
                                      gCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATtg                                                                                                         >  1:267648/1‑127 (MQ=255)
                                                 aaTCTTTCCGCATTTTTGTAATGGTGTTTGGCGGTCAGTTTTAACGATTTTTGAGATTCATAATGGTTAACATTAATACCTGCGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAg                                                                                  <  2:695745/139‑1 (MQ=255)
                                                    cTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAgctg                                                                               <  1:105701/139‑1 (MQ=255)
                                                     tttCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAgctgc                                                                              <  2:291107/139‑1 (MQ=255)
                                                       tCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCtt                                                                            <  1:1428350/139‑1 (MQ=255)
                                                       tCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCtt                                                                            >  1:1269021/1‑139 (MQ=255)
                                                          gCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATtgagca                                                                                                     >  1:679731/1‑111 (MQ=255)
                                                          gCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATtgagca                                                                                                     <  2:679731/111‑1 (MQ=255)
                                                          gCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCt                                                                         <  2:911786/139‑1 (MQ=255)
                                                            aTTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTta                                                                       <  1:959092/139‑1 (MQ=255)
                                                                       gttgttTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAAt                                                            <  1:731076/139‑1 (MQ=255)
                                                                       gttgttTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAAt                                                            <  1:244767/139‑1 (MQ=255)
                                                                             tGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCttt                                                      >  1:412972/1‑139 (MQ=255)
                                                                                cGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAAc                                                                                                                      <  2:1036615/72‑1 (MQ=255)
                                                                                cGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAAc                                                                                                                      >  1:1036615/1‑72 (MQ=255)
                                                                                cGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCt                                                                                                                    >  1:729604/1‑74 (MQ=255)
                                                                                cGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCt                                                                                                                    <  2:729604/74‑1 (MQ=255)
                                                                                  gTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGAt                                                                                                                           <  1:1192191/65‑1 (MQ=255)
                                                                                  gTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGAt                                                                                                                           >  2:1192191/1‑65 (MQ=255)
                                                                                     aGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATAACTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGgtgt                                              >  1:845357/1‑139 (MQ=255)
                                                                                         ttAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATAccc                                                                                          >  1:218809/1‑91 (MQ=255)
                                                                                         ttAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATAccc                                                                                          <  2:325528/91‑1 (MQ=255)
                                                                                         ttAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATAccc                                                                                          <  2:218809/91‑1 (MQ=255)
                                                                                         ttAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATAccc                                                                                          >  1:325528/1‑91 (MQ=255)
                                                                                             ccATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTg                                      <  2:1269021/139‑1 (MQ=255)
                                                                                                 ttttGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGtt                                                                                     >  1:1377620/1‑88 (MQ=255)
                                                                                                 ttttGAGATTCATAATGGTTAACATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGtt                                                                                     <  2:1377620/88‑1 (MQ=255)
                                                                                                                     aaCATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTg                                      >  2:501031/1‑115 (MQ=255)
                                                                                                                     aaCATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTg                                      <  1:501031/115‑1 (MQ=255)
                                                                                                                     aaCATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATc              >  2:1264693/1‑139 (MQ=255)
                                                                                                                      aCATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGgtgt                                              >  1:1142636/1‑106 (MQ=255)
                                                                                                                      aCATTAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGgtgt                                              <  2:1142636/106‑1 (MQ=255)
                                                                                                                          tAATACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATCCAccc         >  2:416386/1‑139 (MQ=255)
                                                                                                                           aaTACCTGATCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTta                                                                       <  1:1223991/76‑1 (MQ=255)
                                                                                                                           aaTACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTta                                                                       >  2:1223991/1‑76 (MQ=255)
                                                                                                                            aTACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATtgagcatgagca                                                                                               >  1:279263/1‑51 (MQ=255)
                                                                                                                            aTACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATtgagcatgagca                                                                                               <  2:1275186/51‑1 (MQ=255)
                                                                                                                            aTACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATtgagcatgagca                                                                                               <  2:279263/51‑1 (MQ=255)
                                                                                                                            aTACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATtgagcatgagca                                                                                               >  1:1275186/1‑51 (MQ=255)
                                                                                                                            aTACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGttt                                       >  1:96363/1‑107 (MQ=255)
                                                                                                                            aTACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGttt                                       <  2:96363/107‑1 (MQ=255)
                                                                                                                            aTACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCt                          >  1:1184190/1‑120 (MQ=255)
                                                                                                                            aTACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATCCACCCCa       >  1:1359607/1‑139 (MQ=255)
                                                                                                                            aTACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATCCACCCCa       >  2:985927/1‑139 (MQ=255)
                                                                                                                            aTACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATCCAACCCa       >  1:635805/1‑139 (MQ=255)
                                                                                                                            aTACCTGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGAATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCt                          <  2:1184190/120‑1 (MQ=255)
                                                                                                                             tACCTGAGCCACTTCTGTAGATCAAACATAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCa                               >  2:989080/1‑114 (MQ=255)
                                                                                                                             tACCTGAGCCACTTCTGTAGATAAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCa                               <  1:989080/114‑1 (MQ=255)
                                                                                                                                 tGAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATCCACCCCAGTTTa  >  2:1444019/1‑139 (MQ=255)
                                                                                                                                  gAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATCCAc           >  2:251488/1‑129 (MQ=255)
                                                                                                                                  gAGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATCCAc           <  1:251488/129‑1 (MQ=255)
                                                                                                                                   aGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCa                                                                  >  2:926180/1‑73 (MQ=255)
                                                                                                                                   aGCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCa                                                                  <  1:926180/73‑1 (MQ=255)
                                                                                                                                    gCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGt                   <  2:351282/119‑1 (MQ=255)
                                                                                                                                    gCCACTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGt                   >  1:351282/1‑119 (MQ=255)
                                                                                                                                        |                                                                                                                                   
CATCTGTTCGGGTGACAGTCGACGTTAATGTGCGGGAAGCCCGGCAAGTAATCTTTCCGCATTTTTGTAATGTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCCCCAGACCTTTGAGGTAGATCCACCCCAGTTTA  >  NC_000913/678828‑679095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A