Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,621,542	G→A	D71N (GAC→AAC)	ydeE →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,621,542	0	G	A	100.0%	151.8 / NA	47	D71N (GAC→AAC)	ydeE	putative transporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/24); total (23/24)

CTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTG  >  NC_000913/1621410‑1621665

cTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGa                                                                                                                       <  1:1409834/139‑1 (MQ=255)

cTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGa                                                                                                                       <  2:614168/139‑1 (MQ=255)

 tGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGaa                                                                                                                      <  2:682349/139‑1 (MQ=255)

             ccATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCtat                                                                                                               <  2:388701/134‑1 (MQ=255)

             ccATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCtat                                                                                                               >  1:388701/1‑134 (MQ=255)

                tttACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCt                                                                                                                 <  2:127360/129‑1 (MQ=255)

                tttACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCt                                                                                                                 >  1:127360/1‑129 (MQ=255)

                               aGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCtt                                                                                        <  2:1288024/139‑1 (MQ=255)

                                     gCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAg                                                                                  <  1:558624/139‑1 (MQ=255)

                                           gtgtCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAg                                                                                                                        <  1:51116/95‑1 (MQ=255)

                                           gtgtCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAg                                                                                                                        >  2:51116/1‑95 (MQ=255)

                                           gtgtCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGttt                                                                            >  1:1037020/1‑139 (MQ=255)

                                                   cTAATCGGTTATGCGATGACAATTGCGCTCACACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGc                                                                      <  1:583934/139‑1 (MQ=255)

                                                    tAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCt                                                                                                                 >  2:368510/1‑93 (MQ=255)

                                                    tAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCt                                                                                                                 <  1:368510/93‑1 (MQ=255)

                                                      aTCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTa                                                                 >  2:767900/1‑139 (MQ=255)

                                                            tATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGa                                                                                                                       <  2:1010710/79‑1 (MQ=255)

                                                            tATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGa                                                                                                                       >  1:1010710/1‑79 (MQ=255)

                                                            tATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCT‑GCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAg                                                                                  <  2:1384748/115‑1 (MQ=255)

                                                            tATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCT‑GCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAg                                                                                  >  1:1384748/1‑115 (MQ=255)

                                                              tGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTg                                                         >  2:1077513/1‑139 (MQ=255)

                                                                cGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACt                                                               <  1:1291922/131‑1 (MQ=255)

                                                                cGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACt                                                               >  2:1291922/1‑131 (MQ=255)

                                                                cGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGaa                                                       <  2:1037020/139‑1 (MQ=255)

                                                                      cAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGt                                                 <  1:767900/139‑1 (MQ=255)

                                                                            cgcT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCt                                           >  1:1233231/1‑139 (MQ=255)

                                                                                      aTTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGc                                   <  1:1105163/139‑1 (MQ=255)

                                                                                            gtcgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCtttt                             <  2:1233231/139‑1 (MQ=255)

                                                                                             tcgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCtat                                                                                                               >  2:1320036/1‑56 (MQ=255)

                                                                                             tcgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCtat                                                                                                               <  1:1320036/56‑1 (MQ=255)

                                                                                             tcgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCttttt                            >  2:1350934/1‑139 (MQ=255)

                                                                                             tcgtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCttttt                            >  1:1017311/1‑139 (MQ=255)

                                                                                             tcgtTTTTAGCCTCGGTTTTGGCATCCTGGCGGATAAGTTCAACAAGaa                                                                                                                      >  1:165340/1‑49 (MQ=255)

                                                                                             tcgtTTTTAGCCTCGGTTTTGGCATCCTGGCGGATAAGTTCAACAAGaa                                                                                                                      <  2:165340/49‑1 (MQ=255)

                                                                                               gtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTa                                                                          >  1:175014/1‑91 (MQ=255)

                                                                                               gtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTa                                                                          <  2:175014/91‑1 (MQ=255)

                                                                                               gtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTg                          >  1:739506/1‑139 (MQ=255)

                                                                                               gtTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGAAGCTGGTTGTGCTCTTTTTTg                          >  1:910829/1‑139 (MQ=255)

                                                                                                 ttttAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGcc                        <  1:1077513/139‑1 (MQ=255)

                                                                                                          cGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACg                                                                                                                   >  2:1071966/1‑39 (MQ=255)

                                                                                                          cGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACg                                                                                                                   <  1:1071966/39‑1 (MQ=38)

                                                                                                          cGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTa                                                                          <  2:14403/80‑1 (MQ=255)

                                                                                                          cGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTa                                                                          >  1:14403/1‑80 (MQ=255)

                                                                                                          cGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAAc               >  2:784303/1‑139 (MQ=255)

                                                                                                          cGGTTTTGGTATCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAAc               >  2:853128/1‑139 (MQ=255)

                                                                                                                    aTCCTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTATCTGCGCCTAtt     <  2:910829/139‑1 (MQ=255)

                                                                                                                       cTGGCGGATAAGTTCAACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTg  >  1:349102/1‑139 (MQ=255)

CTGCCATTTATGACCATTTACTTGAGTCGCCAGTACAGCCTGAGTGTCGATCTAATCGGTTATGCGATGACAATTGCGCT‑‑CACTATTGGCGTCGTTTTTAGCCTCGGTTTTGGTATCCTGGCGGATAAGTTCGACAAGAAACGCTATATGTTACTGGCAATTACCGCCTTCGCCAGCGGTTTTATTGCCATTACTTTAGTGAATAACGTGACGCTGGTTGTGCTCTTTTTTGCCCTCATTAACTGCGCCTATTCTG  >  NC_000913/1621410‑1621665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A