Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F1 I2 R1 777 59.2 2955938 92.5% 2734242 106.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,169,693 C→T pseudogene (475/475 nt) gatR ← pseudogene, repressor for gat operon, interrupted by IS3, split galactitol utilization operon repressor, fragment 2, split galactitol utilization operon repressor, interrupted

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,169,6930CT100.0% 178.5 / NA 55pseudogene (475/475 nt)gatRpseudogene, repressor for gat operon, interrupted by IS3, split galactitol utilization operon repressor, fragment 2, split galactitol utilization operon repressor, interrupted
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/26);  total (29/26)

GGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTT  >  NC_000913/2169563‑2169824
                                                                                                                                  |                                                                                                                                   
ggCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGt                                                                                                                             >  1:459993/1‑139 (MQ=255)
     cgttgcgttgTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGtaatta                                                                                                                        >  2:662325/1‑139 (MQ=255)
        tgcgttgTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTaattaatt                                                                                                                     >  2:665222/1‑139 (MQ=255)
                                gTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCa                                                                                             >  2:300285/1‑139 (MQ=255)
                                 tCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGtaa                                                                                                                           <  2:35856/108‑1 (MQ=255)
                                 tCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGtaa                                                                                                                           >  1:35856/1‑108 (MQ=255)
                                 tCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAg                                                                                            >  2:877420/1‑139 (MQ=255)
                                       gTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATc                                                                                      <  2:1079318/139‑1 (MQ=255)
                                       gTTGCGTTAATCAAACTATTCTCTCTTAAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATc                                                                                      <  1:300285/139‑1 (MQ=255)
                                        ttGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATcc                                                                                     <  2:459993/139‑1 (MQ=255)
                                           cGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATTCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCAcc                                                                                  >  1:1039696/1‑139 (MQ=255)
                                                  cAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGaca                                                                           <  2:473363/139‑1 (MQ=255)
                                                          tctctcTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTAccc                                                                                                               >  1:1474557/1‑95 (MQ=255)
                                                          tctctcTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTAccc                                                                                                               <  2:1474557/95‑1 (MQ=255)
                                                          tctctcTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTAccc                                                                                                               >  1:732681/1‑95 (MQ=255)
                                                          tctctcTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTAccc                                                                                                               <  2:732681/95‑1 (MQ=255)
                                                            tctctTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCatcatc                                                                 <  1:665222/139‑1 (MQ=255)
                                                            tctctTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCatcatc                                                                 >  2:470668/1‑139 (MQ=255)
                                                            tctctTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCatcatc                                                                 <  1:877420/139‑1 (MQ=255)
                                                              tctTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCgg                                                               <  1:662325/139‑1 (MQ=255)
                                                                   aaCCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCa                                                                                             <  1:1443356/104‑1 (MQ=255)
                                                                   aaCCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCa                                                                                             >  2:1443356/1‑104 (MQ=255)
                                                                        gggAGAATCCGTAATGCCGGATGTGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTaataataat                                                     >  1:584237/1‑139 (MQ=255)
                                                                        gggAGAATCCGTAATGCCGGATGCGGTGTCAACATCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTCATCCACCTCTGACACAGCATCATCGGTactaataat                                                     >  1:1255317/1‑139 (MQ=255)
                                                                        gggAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGt                                                                                           <  1:265189/101‑1 (MQ=255)
                                                                        gggAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGt                                                                                           >  2:265189/1‑101 (MQ=255)
                                                                        gggAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGca                                                                      >  1:889568/1‑122 (MQ=255)
                                                                        gggAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGca                                                                      <  2:889568/122‑1 (MQ=255)
                                                                             aaTCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGc                                                                       >  1:710488/1‑116 (MQ=255)
                                                                             aaTCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGc                                                                       <  2:710488/116‑1 (MQ=255)
                                                                                  gTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAAttttt                                           <  2:1316794/139‑1 (MQ=255)
                                                                                      tGCCGGATGTGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCGCCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGa                                       <  2:753277/139‑1 (MQ=255)
                                                                                      tGCCGGATGTGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGa                                       <  2:584237/139‑1 (MQ=255)
                                                                                       gCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATc                                                                                      >  1:951020/1‑91 (MQ=255)
                                                                                       gCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATc                                                                                      <  2:951020/91‑1 (MQ=255)
                                                                                          ggATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTAccc                                                                                                               <  2:954510/63‑1 (MQ=255)
                                                                                          ggATGCGGTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTAccc                                                                                                               >  1:954510/1‑63 (MQ=255)
                                                                                                ggTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGa                                       >  2:589472/1‑129 (MQ=255)
                                                                                                ggTGTAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGa                                       <  1:589472/129‑1 (MQ=255)
                                                                                                  tgtAAACACCTTATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCt                           >  1:683973/1‑139 (MQ=255)
                                                                                                            ttATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTg                                                                                                     >  2:646425/1‑55 (MQ=255)
                                                                                                            ttATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTg                                                                                                     <  1:646425/55‑1 (MQ=255)
                                                                                                            ttATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAAcc                 >  1:132721/1‑139 (MQ=255)
                                                                                                            ttATCCGGCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAAcc                 >  1:585559/1‑139 (MQ=255)
                                                                                                                   gCATACAGCACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGc                                                                                                    <  2:895145/49‑1 (MQ=255)
                                                                                                                   gCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGc                                                                                                    >  1:895145/1‑49 (MQ=255)
                                                                                                                   gCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGcatcat                                                                  >  2:16334/1‑83 (MQ=255)
                                                                                                                   gCATACAGAACAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGcatcat                                                                  <  1:16334/83‑1 (MQ=255)
                                                                                                                           aaCAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCa                                                                                             <  1:1002766/48‑1 (MQ=255)
                                                                                                                           aaCAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCa                                                                                             >  2:1002766/1‑48 (MQ=255)
                                                                                                                           aaCAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAAttt  >  1:695644/1‑139 (MQ=255)
                                                                                                                             cAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGa                                       >  2:742459/1‑100 (MQ=255)
                                                                                                                             cAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGa                                       <  1:742459/100‑1 (MQ=255)
                                                                                                                             cAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACcgc               <  2:753422/124‑1 (MQ=255)
                                                                                                                             cAATATTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACcgc               >  1:753422/1‑124 (MQ=255)
                                                                                                                                  |                                                                                                                                   
GGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTT  >  NC_000913/2169563‑2169824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A