Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,398,919	G→A	A416V (GCG→GTG)	nuoG ←	NADH:ubiquinone oxidoreductase, chain G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,398,919	0	G	A	100.0%	67.6 / NA	22	A416V (GCG→GTG)	nuoG	NADH:ubiquinone oxidoreductase, chain G
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/11); total (11/11)

GATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGCCCAGCACCAGTACCGCATCGTAA  >  NC_000913/2398783‑2399053

gATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCcaca                                                                                                                                      <  1:772117/139‑1 (MQ=255)

                   gcCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACtt                                                                                                                   >  1:650396/1‑139 (MQ=255)

                                   gTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCAt                                                                                                   >  2:1283099/1‑139 (MQ=255)

                                    tGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCAtt                                                                                                  >  2:1187642/1‑139 (MQ=255)

                                                  aCGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTAcc                                                                                    <  2:296431/139‑1 (MQ=255)

                                                       gcgATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTca                                                                               <  1:1427713/139‑1 (MQ=255)

                                                                                      tGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGAcgcgcgc                                                >  1:531887/1‑139 (MQ=255)

                                                                                       ggTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGAcgcgcgcg                                               <  2:379111/139‑1 (MQ=255)

                                                                                                         cTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCt                             <  2:1460205/139‑1 (MQ=255)

                                                                                                          ttCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCtt                            <  2:650396/139‑1 (MQ=255)

                                                                                                               aCGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGccc                       <  1:1187642/139‑1 (MQ=255)

                                                                                                               aCGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGccc                       <  2:347071/139‑1 (MQ=255)

                                                                                                                   tGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGCCCAGCa                   >  1:162168/1‑139 (MQ=255)

                                                                                                                    gACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTcg                                                                                               >  1:1247520/1‑62 (MQ=255)

                                                                                                                    gACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTcg                                                                                               <  2:1247520/62‑1 (MQ=255)

                                                                                                                               aGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGccc                       <  1:749886/123‑1 (MQ=255)

                                                                                                                               aGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGccc                       >  2:749886/1‑123 (MQ=255)

                                                                                                                                    tGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTAc                                                                                     >  2:223763/1‑56 (MQ=255)

                                                                                                                                    tGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTAc                                                                                     <  1:223763/56‑1 (MQ=255)

                                                                                                                                    tGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGc                                                                           >  1:643102/1‑66 (MQ=255)

                                                                                                                                    tGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGc                                                                           <  2:643102/66‑1 (MQ=255)

                                                                                                                                    tGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGCCCAGCACCAGTACCGCATCGTaa  >  2:1248253/1‑139 (MQ=255)

                                                                                                                                    tGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGCCCAGCACCAGTACCGCATCGTaa  >  2:27844/1‑139 (MQ=255)

GATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGCCCAGCACCAGTACCGCATCGTAA  >  NC_000913/2398783‑2399053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A