Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
781 |
46.9 |
2231650 |
93.5% |
2086592 |
109.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,398,919 |
G→A |
A416V (GCG→GTG) |
nuoG ← |
NADH:ubiquinone oxidoreductase, chain G |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,398,919 | 0 | G | A | 100.0%
| 138.0
/ NA
| 43 | A416V (GCG→GTG) | nuoG | NADH:ubiquinone oxidoreductase, chain G |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/21); total (22/21) |
ATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGCCCAGCACCAGTACCGCATCGTAA > NC_000913/2398784‑2399053
|
aTGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACaa > 2:941943/1‑139 (MQ=255)
aaCGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCAGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGc < 1:154767/139‑1 (MQ=255)
ccGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGAACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTgccgc > 2:680894/1‑139 (MQ=255)
ggTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCa > 1:805506/1‑139 (MQ=255)
gTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGtgc < 1:650574/130‑1 (MQ=255)
gTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGtgc > 2:650574/1‑130 (MQ=255)
gTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCAt > 2:1005186/1‑139 (MQ=255)
tGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGt > 1:950962/1‑113 (MQ=255)
tGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCAtt > 2:665131/1‑139 (MQ=255)
tGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCAtt > 2:600395/1‑139 (MQ=255)
tGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGACGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGt < 2:950962/113‑1 (MQ=255)
aGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCt < 2:982682/139‑1 (MQ=255)
gTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTc < 1:428329/131‑1 (MQ=255)
gTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTc > 2:428329/1‑131 (MQ=255)
cACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGTGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTAc < 2:613725/139‑1 (MQ=255)
tcatcCAGAGGGGTGTCATCAACGTTGGGAAGAAACAGTGGATTCTTTGTACGTGGACGGGTGTTGAGGGGGGCAAAAATCTGTGAGTCAGCCTCTTTGTGTGCAACCGCTATTTCGCGCGCAGTGCCTTTCACAGCCt < 2:1348/139‑1 (MQ=255)
aCGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACt > 1:962333/1‑139 (MQ=255)
ggTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGcc < 1:633593/139‑1 (MQ=255)
tgtCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAg > 1:676685/1‑139 (MQ=255)
cGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTcg < 2:773539/95‑1 (MQ=255)
cGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTcg > 1:773539/1‑95 (MQ=255)
tGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCt > 2:683024/1‑98 (MQ=255)
tGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCt < 1:683024/98‑1 (MQ=255)
tGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGAcgcgcgc > 1:603699/1‑139 (MQ=255)
tGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGAcgcgcgc > 2:740041/1‑139 (MQ=255)
ggTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTAc < 1:896617/101‑1 (MQ=255)
ggTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTAc > 2:896617/1‑101 (MQ=255)
ggTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGGCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTATACCTTTCACAGCCTGACGCACTGCCAGCGCGAcgcgcgcc < 1:684359/139‑2 (MQ=255)
caaacaGCGGATGCCTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCTCGTGCTGTGCTGCCGCCATTTCGCTCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCACGCCGGt < 1:680894/139‑1 (MQ=255)
ggATGCTTCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTaa < 2:603699/139‑1 (MQ=255)
ttCGCACGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCtt < 1:740041/139‑1 (MQ=255)
aCGTTGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGccc < 2:962333/139‑1 (MQ=255)
tGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTcg > 2:972367/1‑63 (MQ=255)
tGACCGATGTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTcg < 1:972367/63‑1 (MQ=255)
gTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGtgc < 1:920999/42‑1 (MQ=255)
gTTGAGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGtgc > 2:920999/1‑42 (MQ=255)
aGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGt < 2:358748/110‑1 (MQ=255)
aGGATTGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGt > 1:358748/1‑110 (MQ=255)
tGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTcgc < 1:914375/47‑1 (MQ=255)
tGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTcgc > 2:914375/1‑47 (MQ=255)
tGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGCCCAGCAc > 2:215296/1‑123 (MQ=255)
tGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGCCCAGCAc < 1:215296/123‑1 (MQ=255)
tGCCACAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGCCCAGCACCAGTACCGCATCGTaa > 2:896390/1‑139 (MQ=255)
|
ATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTACCTTTCACAGCCTGACGCACTGCCAGCGCGACGCGCGCGCCGGTCTGGGTAACGTCTTCGCCCAGCACCAGTACCGCATCGTAA > NC_000913/2398784‑2399053
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
