Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,742,962	G→A	D194N (GAT→AAT)	yfiN →	putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,742,962	0	G	A	100.0%	117.3 / NA	37	D194N (GAT→AAT)	yfiN	putative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/19); total (18/19)

ACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTA  >  NC_000913/2742828‑2743096

aCAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGt                                                                                                                                    <  2:1006505/139‑1 (MQ=255)

aCAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGt                                                                                                                                    <  1:170941/139‑1 (MQ=255)

aCAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGt                                                                                                                                    <  1:8393/139‑1 (MQ=255)

        aTCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACatg                                                                                                                            <  2:222438/139‑1 (MQ=255)

           aGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACatgatg                                                                                                                         <  2:1473672/139‑1 (MQ=255)

            gCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGAtgt                                                                                                                        >  1:95495/1‑139 (MQ=255)

             ccATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGAtgtg                                                                                                                       >  1:1450137/1‑139 (MQ=255)

                   tATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTcc                                                                                                                 <  2:1450137/139‑1 (MQ=255)

                       tGGTTTTCACTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAAcc                                                                                                             <  1:555893/139‑1 (MQ=255)

                                       tACTGACCGGTTGTATTCTGCTGGTATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcgacg                                                                                             <  2:821844/139‑1 (MQ=255)

                                       tACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcgacg                                                                                             <  1:654008/139‑1 (MQ=255)

                                            aCCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCTTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGttt                                                                                        <  1:1461568/139‑1 (MQ=255)

                                            aCCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcga                                                                                               >  1:737524/1‑132 (MQ=255)

                                            aCCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcga                                                                                               <  2:737524/132‑1 (MQ=255)

                                            aCCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGttt                                                                                        <  1:155253/139‑1 (MQ=255)

                                               ggTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCgg                                                                                     >  1:812621/1‑139 (MQ=255)

                                                           ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCg                                                                         >  2:555371/1‑139 (MQ=255)

                                                           ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCg                                                                         >  1:775867/1‑139 (MQ=255)

                                                                   aGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGttt                                                                 >  2:596380/1‑139 (MQ=255)

                                                                     gCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcga                                                                                               <  1:63062/107‑1 (MQ=255)

                                                                     gCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcga                                                                                               >  2:63062/1‑107 (MQ=255)

                                                                        tCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCg                                                            >  1:831638/1‑139 (MQ=255)

                                                                                      aCCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACt                                              >  1:21418/1‑139 (MQ=255)

                                                                                              tttGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGt                                      <  2:944294/139‑1 (MQ=255)

                                                                                                 gCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTctg                                   <  1:555371/139‑1 (MQ=255)

                                                                                                   acaATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTctgct                                 <  2:812621/139‑1 (MQ=255)

                                                                                                         gCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcgacg                                                                                             <  2:1383907/73‑1 (MQ=255)

                                                                                                         gCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcgacg                                                                                             >  1:1383907/1‑73 (MQ=255)

                                                                                                          ccTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGaa                          >  1:1237046/1‑139 (MQ=255)

                                                                                                            tGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAAt                        >  1:1475408/1‑139 (MQ=255)

                                                                                                                    gcgcTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGaa                          >  2:25766/1‑129 (MQ=255)

                                                                                                                    gcgcTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGaa                          <  1:25766/129‑1 (MQ=255)

                                                                                                                    gcgcTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGt                >  1:915765/1‑139 (MQ=255)

                                                                                                                        tGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCaa                                          >  2:1297365/1‑109 (MQ=255)

                                                                                                                        tGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCaa                                          <  1:1297365/109‑1 (MQ=255)

                                                                                                                        tGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCa            >  2:141373/1‑139 (MQ=255)

                                                                                                                                  cACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTa  >  2:1127245/1‑139 (MQ=255)

ACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTA  >  NC_000913/2742828‑2743096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A