Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I1 R1 757 76.1 3598070 93.4% 3360597 108.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,742,962 G→A D194N (GAT→AAT)  yfiN → putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,742,9620GA100.0% 188.0 / NA 59D194N (GAT→AAT) yfiNputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/30);  total (29/30)

ACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTA  >  NC_000913/2742828‑2743096
                                                                                                                                      |                                                                                                                                      
aCAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGt                                                                                                                                    <  1:926089/139‑1 (MQ=255)
  aGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCg                                                                                                                                  <  1:922185/139‑1 (MQ=255)
       aaTCAGCCATTTTATCTGTTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCAACGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACat                                                                                                                             <  1:638364/139‑1 (MQ=255)
           aGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACatgatg                                                                                                                         <  2:872531/139‑1 (MQ=255)
             ccATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGAtgtg                                                                                                                       >  1:1740140/1‑139 (MQ=255)
             ccATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGAtgtg                                                                                                                       >  1:981467/1‑139 (MQ=255)
                                           gACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGtt                                                                                         <  1:425946/139‑1 (MQ=255)
                                             ccGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTc                                                                                       >  1:1206529/1‑139 (MQ=255)
                                               ggTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCgg                                                                                     >  2:580265/1‑139 (MQ=255)
                                                 ttGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGgaa                                                                                   >  1:196206/1‑139 (MQ=255)
                                                      ttCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAAca                                                                              >  1:533884/1‑138 (MQ=255)
                                                         tgctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcgacg                                                                                             <  1:337096/121‑1 (MQ=255)
                                                         tgctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcgacg                                                                                             >  2:337096/1‑121 (MQ=255)
                                                           atgGCATCAGGCATCACAATTACCCTCACTCGCCCGTTGCACAATTGCCTGGTAGACGCGCTGAACAGTATCACCAACGTCGTACGTGATTTGCATACCACCCGCACTTTTTCCCGACCCGTTTCGGAAGAACGTATCg                                                                         >  1:1152675/2‑139 (MQ=255)
                                                                   aGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGttt                                                                 <  2:196206/139‑1 (MQ=255)
                                                                     gCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCt                                                                       <  2:1584442/131‑1 (MQ=255)
                                                                     gCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCt                                                                       >  1:1584442/1‑131 (MQ=255)
                                                                        tCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCg                                                                                                                                  <  1:917792/69‑1 (MQ=255)
                                                                        tCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCg                                                                                                                                  >  2:917792/1‑69 (MQ=255)
                                                                          gCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCt                                                          <  1:30223/139‑1 (MQ=255)
                                                                                aCCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGctc                                                    <  2:1740140/139‑1 (MQ=255)
                                                                                  ccTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCtctc                                                  <  2:394135/139‑1 (MQ=255)
                                                                                  ccTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCtctc                                                  <  1:580265/139‑1 (MQ=255)
                                                                                      aCCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACt                                              >  2:1039698/1‑139 (MQ=255)
                                                                                           ccATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAAc                                         >  2:20151/1‑139 (MQ=255)
                                                                                                  cacaATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTctgc                                  >  1:770654/1‑139 (MQ=255)
                                                                                                    caATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTctgctg                                <  2:770654/139‑1 (MQ=255)
                                                                                                      aTGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGa                              <  2:1563140/139‑1 (MQ=255)
                                                                                                      aTGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGa                              >  1:255531/1‑139 (MQ=255)
                                                                                                       tGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGa                              <  2:439523/138‑1 (MQ=255)
                                                                                                       tGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGa                              >  1:439523/1‑138 (MQ=255)
                                                                                                          ccTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAg                                                                    >  1:176944/1‑97 (MQ=255)
                                                                                                          ccTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAg                                                                    <  2:176944/97‑1 (MQ=255)
                                                                                                          ccTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGa                              <  2:633534/135‑1 (MQ=255)
                                                                                                          ccTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGaa                          >  2:39907/1‑139 (MQ=255)
                                                                                                          ccTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGGTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGa                              >  1:633534/1‑135 (MQ=255)
                                                                                                           cTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGaaa                         <  2:517607/139‑1 (MQ=255)
                                                                                                               tAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGa                     >  2:1125512/1‑139 (MQ=255)
                                                                                                                 gAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGgaa                                                                                   <  1:1609717/75‑1 (MQ=255)
                                                                                                                 gAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGgaa                                                                                   >  2:1609717/1‑75 (MQ=255)
                                                                                                                 gAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACATCAACAGTCTGCTGGATGAAATGGAag                   <  2:1152675/139‑1 (MQ=255)
                                                                                                                   aGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcgacg                                                                                             >  1:523373/1‑63 (MQ=255)
                                                                                                                   aGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcgacg                                                                                             <  2:523373/63‑1 (MQ=255)
                                                                                                                   aGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCt                                                          <  2:818465/98‑1 (MQ=255)
                                                                                                                   aGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCt                                                          >  1:818465/1‑98 (MQ=255)
                                                                                                                    gcgcTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCt                                                                       >  2:1380373/1‑84 (MQ=255)
                                                                                                                    gcgcTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCt                                                                       <  1:1380373/84‑1 (MQ=255)
                                                                                                                     cgcTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTg               <  1:39907/139‑1 (MQ=255)
                                                                                                                       cTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGaact                        <  1:1079064/128‑3 (MQ=255)
                                                                                                                       cTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAAt                        >  2:1079064/1‑128 (MQ=255)
                                                                                                                        tGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTg                                                                      <  1:1078184/81‑1 (MQ=255)
                                                                                                                        tGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTg                                                                      >  2:1078184/1‑81 (MQ=255)
                                                                                                                        tGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCa            >  2:1695665/1‑139 (MQ=255)
                                                                                                                        tGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCa            >  2:1499450/1‑139 (MQ=255)
                                                                                                                        tGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCa            >  1:50260/1‑139 (MQ=255)
                                                                                                                                atCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCaa                                          >  2:1267928/1‑101 (MQ=255)
                                                                                                                                atCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCCGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCaa                                          <  1:1267928/101‑1 (MQ=255)
                                                                                                                                  cACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTa  <  2:255531/139‑1 (MQ=255)
                                                                                                                                  cACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTa  <  1:20151/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                      
ACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTA  >  NC_000913/2742828‑2743096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A