Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I2 R1
|
777 |
59.2 |
2955938 |
92.5% |
2734242 |
106.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,565,464 |
C→A |
L129F (TTG→TTT) |
yjiM ← |
putative 2‑hydroxyglutaryl‑CoA dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,565,464 | 0 | C | A | 100.0%
| 148.4
/ NA
| 46 | L129F (TTG→TTT) | yjiM | putative 2‑hydroxyglutaryl‑CoA dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (18/28); total (18/28) |
TTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGG > NC_000913/4565332‑4565572
|
tttCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGc < 2:1159622/139‑1 (MQ=255)
tttCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGc < 2:1149763/139‑1 (MQ=255)
cgcAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTaa > 2:495938/1‑139 (MQ=255)
aTCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGcc < 2:510283/139‑1 (MQ=255)
aTCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGcc < 2:124765/139‑1 (MQ=255)
tCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCa < 2:970899/139‑1 (MQ=255)
tCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCa < 1:212811/139‑1 (MQ=255)
ttCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATg < 2:748106/139‑1 (MQ=255)
ttCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATg < 1:495938/139‑1 (MQ=255)
cTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGc > 2:386315/1‑105 (MQ=255)
cTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGc < 1:386315/105‑1 (MQ=255)
tacccAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCAt > 2:725926/3‑133 (MQ=255)
tacccAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCAt < 1:725926/131‑1 (MQ=255)
tGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGaa < 1:557546/106‑1 (MQ=255)
tGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGaa > 2:557546/1‑106 (MQ=255)
tGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACAttt > 2:837288/1‑139 (MQ=255)
tcttctACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGcc > 2:928441/1‑137 (MQ=255)
tcttctACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGcc < 1:928441/137‑1 (MQ=255)
cttctACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTc > 2:1262546/1‑139 (MQ=255)
ctACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCa < 1:837288/139‑1 (MQ=255)
aCCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAgg < 2:61135/139‑1 (MQ=255)
ccGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTAtt < 1:173247/111‑1 (MQ=255)
ccGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTAtt > 2:173247/1‑111 (MQ=255)
ttttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGc > 2:59142/1‑74 (MQ=255)
ttttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGc < 1:59142/74‑1 (MQ=255)
ttttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGaa < 2:1013607/84‑1 (MQ=255)
ttttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGaa > 1:1013607/1‑84 (MQ=255)
gcagcaTCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGaccac < 2:1315615/139‑1 (MQ=255)
tcGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCg < 1:235595/139‑1 (MQ=255)
cGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACt > 1:1309682/1‑75 (MQ=255)
cGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACt < 2:1309682/75‑1 (MQ=255)
ggCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGaa < 2:728351/139‑1 (MQ=255)
tCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGAccacca > 2:315691/1‑126 (MQ=255)
tCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGAccacca < 1:315691/126‑1 (MQ=255)
cATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAaagtaga > 2:561665/1‑139 (MQ=255)
acgcGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGaa > 2:517812/1‑47 (MQ=255)
acgcGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGaa < 1:517812/47‑1 (MQ=255)
acgcGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAgggg < 1:561665/139‑1 (MQ=255)
cTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCg < 1:1115868/100‑1 (MQ=255)
cTGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCg > 2:1115868/1‑100 (MQ=255)
tGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAggg > 2:242334/1‑115 (MQ=255)
tGTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAggg < 1:242334/115‑1 (MQ=255)
gTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCAt < 1:223383/50‑1 (MQ=255)
gTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCAt > 2:223383/1‑50 (MQ=255)
gTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCa > 1:593869/1‑82 (MQ=255)
gTTGGGAAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCa < 2:593869/82‑1 (MQ=255)
|
TTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCATCACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGG > NC_000913/4565332‑4565572
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
