Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
765 |
53.2 |
2691310 |
93.4% |
2513683 |
103.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,565,464 |
C→A |
L129F (TTG→TTT) |
yjiM ← |
putative 2‑hydroxyglutaryl‑CoA dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,565,464 | 0 | C | A | 100.0%
| 136.6
/ NA
| 44 | L129F (TTG→TTT) | yjiM | putative 2‑hydroxyglutaryl‑CoA dehydratase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (23/21); total (23/21) |
TTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGC > NC_000913/4565332‑4565588
|
tttCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGc < 2:244959/139‑1 (MQ=255)
tGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAAc > 1:725292/1‑139 (MQ=255)
aTCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGcc < 1:1191914/139‑1 (MQ=255)
ttCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATg < 2:725292/139‑1 (MQ=255)
cGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTa > 2:594307/1‑139 (MQ=255)
cTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCa > 2:627669/1‑133 (MQ=255)
cTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCa < 1:627669/133‑1 (MQ=255)
gTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCAt > 2:966673/1‑126 (MQ=255)
gTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCAt < 1:966673/126‑1 (MQ=255)
gTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACAtt > 1:1004403/1‑139 (MQ=255)
gTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACAtt > 2:175910/1‑139 (MQ=255)
tGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACAttt > 1:862109/1‑139 (MQ=255)
cccAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTaaa > 2:904236/1‑114 (MQ=255)
cccAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTaaa < 1:904236/114‑1 (MQ=255)
cAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGaa > 1:415005/1‑102 (MQ=255)
cAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGaa < 2:415005/102‑1 (MQ=255)
tcttctACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGaa > 2:204204/1‑94 (MQ=255)
tcttctACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGaa < 1:204204/94‑1 (MQ=255)
tcttctACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATg < 1:344175/114‑1 (MQ=255)
tcttctACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATg > 2:344175/1‑114 (MQ=255)
tcttctACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGt < 1:381840/139‑1 (MQ=255)
tACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAg > 2:517409/1‑139 (MQ=255)
aCCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAgg < 1:517409/139‑1 (MQ=255)
ccGTATTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTCTTCATACATTTTCTTTTTGCCGTCGCAggt > 2:693495/1‑139 (MQ=255)
ttttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGaa < 1:1094412/84‑1 (MQ=255)
ttttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGaa > 2:1094412/1‑84 (MQ=255)
ttttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACt < 1:208551/96‑1 (MQ=255)
ttttttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACt > 2:208551/1‑96 (MQ=255)
ttGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCa < 1:175910/139‑1 (MQ=255)
cGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCa < 1:135299/112‑1 (MQ=255)
cGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCa > 2:135299/1‑112 (MQ=255)
cTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGaaaa < 2:1004403/139‑1 (MQ=255)
cATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACt > 1:401923/1‑67 (MQ=255)
cATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACt < 2:401923/67‑1 (MQ=255)
cATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGt > 2:181257/1‑100 (MQ=255)
cATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGt < 1:181257/100‑1 (MQ=255)
cgcacgcGAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAaagtagaagtag < 1:594307/139‑1 (MQ=255)
cgcgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGc > 2:415562/1‑139 (MQ=255)
gcgAGGCATCGTCCTTAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCa > 2:148492/1‑139 (MQ=255)
ggCATCGTCCTTAACGCTGTTGGGAAATTGCATCACACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGaa > 2:563658/1‑120 (MQ=255)
ggCATCGTCCTTAACGCTGTTGGGAAATTGCATCACACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGaa < 1:563658/120‑1 (MQ=255)
ttAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGa < 1:1008739/94‑1 (MQ=255)
ttAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGc > 2:1333317/1‑139 (MQ=255)
ttAACGCTGTTGGGAAATTGCAT‑‑CACATGAACAGGCCTAAACTCCGCCATATATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGa > 2:1008739/1‑94 (MQ=255)
|
TTTCAGCGCAATGGCATCGCGCAGAGCATCTTCGCTAATCTCGTGCCCAAAACGTTCTTCTACCGTTTTTTGCAAGCGCAGCATCTCGGCTTTCCATAACGCACGCGAGGCATCGTCCTTAACGCTGTTGGGCAATTGCAT‑‑CACATGAACAGGCTTAAACTCCGCCATGTATTCATACATTTTCTTTTTGCCGTCGCAGGTGGTTTCACCGACCACCAGATCCGAAAAGTAGAAGTAGGGGCATTTATCGGTTTTGC > NC_000913/4565332‑4565588
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
