Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,092,286	A→G	Y2H (TAT→CAT)	pgaA ←	biofilm adhesin polysaccharide PGA secretin, OM porin, poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,092,286	0	A	G	100.0%	343.5 / NA	94	Y2H (TAT→CAT)	pgaA	biofilm adhesin polysaccharide PGA secretin, OM porin, poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine export protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (44/50); total (44/50)

GCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAGA  >  NC_000913/1092152‑1092420

gCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc                                                                                                                                    <  2:442822/139‑1 (MQ=255)

gCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc                                                                                                                                    >  2:1530515/1‑139 (MQ=255)

gCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc                                                                                                                                    >  1:442822/1‑139 (MQ=255)

gCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc                                                                                                                                    <  2:912337/139‑1 (MQ=255)

 cGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATcc                                                                                                                                   <  1:289101/139‑1 (MQ=255)

     cTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc                                                                                                                                    <  1:1148340/134‑1 (MQ=255)

     cTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc                                                                                                                                    >  2:1148340/1‑134 (MQ=255)

     cTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTa                                                                                                                               <  1:1192413/139‑1 (MQ=255)

     cTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTa                                                                                                                               <  1:697855/139‑1 (MQ=255)

     cTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTa                                                                                                                               <  1:708960/139‑1 (MQ=255)

      ttCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTAt                                                                                                                              <  1:1096310/139‑1 (MQ=255)

            aatCAATGCATCATAGGCGTTATTAACAGGACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTcc                                                                                                                        <  2:1546319/139‑1 (MQ=255)

                aaTGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAACGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGt                                                                                                                    >  1:404429/1‑139 (MQ=255)

                 aTGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTa                                                                                                                   <  2:404429/139‑1 (MQ=255)

                  tGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTAt                                                                                                                  >  2:1503467/1‑139 (MQ=255)

                  tGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTAt                                                                                                                  >  1:1598737/1‑139 (MQ=255)

                     atcatAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGc                                                                                                               >  2:920830/1‑139 (MQ=255)

                       catAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCa                                                                                                             >  2:330882/1‑139 (MQ=255)

                       catAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCa                                                                                                             >  2:597217/1‑139 (MQ=255)

                       catAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCa                                                                                                             >  1:420769/1‑139 (MQ=255)

                         tAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCaaa                                                                                                           >  1:965691/1‑139 (MQ=255)

                         tAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCaaa                                                                                                           >  2:722259/1‑139 (MQ=255)

                            gCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtc                                                                                                        >  1:982516/1‑139 (MQ=255)

                             cGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtct                                                                                                       <  2:420769/139‑1 (MQ=255)

                             cGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtct                                                                                                       <  1:722259/139‑1 (MQ=255)

                              gTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctc                                                                                                      <  1:1530515/139‑1 (MQ=255)

                               ttattaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctct                                                                                                     >  1:1613202/1‑139 (MQ=255)

                                 attaACAGCACTCTTCGCCTCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCATAGCCCATTTGCTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATcc                                                                                                                                   <  2:1025711/107‑1 (MQ=255)

                                 attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATcc                                                                                                                                   >  1:1025711/1‑107 (MQ=255)

                                 attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTAt                                                                                                                              <  2:1582932/112‑1 (MQ=255)

                                 attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTAt                                                                                                                              >  1:1582932/1‑112 (MQ=255)

                                 attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                   >  2:1455167/1‑139 (MQ=255)

                                 attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                   <  2:965691/139‑1 (MQ=255)

                                 attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                   >  1:663040/1‑139 (MQ=255)

                                 attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                   <  1:330882/139‑1 (MQ=255)

                                 attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                   >  2:1293442/1‑139 (MQ=255)

                                 attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                   <  2:663040/139‑1 (MQ=255)

                                 attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                   <  1:1293442/139‑1 (MQ=255)

                                 attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                   <  2:1598737/139‑1 (MQ=255)

                                 attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                   >  1:1465964/1‑139 (MQ=255)

                                 attaACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                   <  2:1613202/139‑1 (MQ=255)

                                    aaCAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTg                                                                                                <  1:1455167/139‑1 (MQ=255)

                                       aGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt                                                                                             >  2:190184/1‑139 (MQ=255)

                                       aGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt                                                                                             <  1:597217/139‑1 (MQ=255)

                                          aCTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt                                                                                             <  2:852839/136‑1 (MQ=255)

                                          aCTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt                                                                                             >  1:852839/1‑136 (MQ=255)

                                            tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc                                                                                                                                    <  2:613594/95‑1 (MQ=255)

                                            tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc                                                                                                                                    >  1:613594/1‑95 (MQ=255)

                                            tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTg                                                                                                <  2:838646/131‑1 (MQ=255)

                                            tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTg                                                                                                >  1:838646/1‑131 (MQ=255)

                                            tctTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAAt                                                                                        >  2:424932/1‑139 (MQ=255)

                                              ttCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCa                                                                                                                                      >  2:881115/1‑91 (MQ=255)

                                              ttCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTa                                                                                      <  1:1503467/139‑1 (MQ=255)

                                                cgccgcGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACa                                                                                    >  1:823517/1‑139 (MQ=255)

                                                cgccgcGGGACTCACTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc                                                                                                                                    >  1:1049822/1‑91 (MQ=255)

                                                cgccgcGGGACTCACTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc                                                                                                                                    <  2:1049822/91‑1 (MQ=255)

                                                   cgcgGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTa                                                                                                                               <  2:855594/93‑1 (MQ=255)

                                                   cgcgGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTa                                                                                                                               >  1:855594/1‑93 (MQ=255)

                                                        gACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTa                                                                                                                               <  1:291963/88‑1 (MQ=255)

                                                        gACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTa                                                                                                                               >  2:291963/1‑88 (MQ=255)

                                                          cTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTc                                                                                                                         >  2:892926/1‑92 (MQ=255)

                                                          cTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTc                                                                                                                         <  1:892926/92‑1 (MQ=255)

                                                                  tAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt                                                                                             >  1:1567303/1‑112 (MQ=255)

                                                                  tAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt                                                                                             <  2:1567303/112‑1 (MQ=255)

                                                                  tAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAaagaaaga                                                                  <  2:823517/139‑1 (MQ=255)

                                                                    aaaaTGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCa                                                                                                                                      <  2:1437069/69‑1 (MQ=255)

                                                                         gCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATAttttt                                                           >  2:89175/1‑139 (MQ=255)

                                                                         gCGGCAGTAAGAAGGTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATAttt                                                             <  1:1265943/137‑1 (MQ=255)

                                                                         gCGGCAGTAAGAAGGTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATAttt                                                             >  2:1265943/1‑137 (MQ=255)

                                                                           ggCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                   >  2:8574/1‑97 (MQ=255)

                                                                           ggCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAAtctctct                                                                                                   <  1:8574/97‑1 (MQ=255)

                                                                           ggCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAaagaaaga                                                                  >  2:372524/1‑130 (MQ=255)

                                                                           ggCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAaagaaaga                                                                  <  1:372524/130‑1 (MQ=255)

                                                                           ggCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGc                                                         <  1:920830/139‑1 (MQ=255)

                                                                               gTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTa                                                                                                                               >  2:318136/1‑65 (MQ=255)

                                                                               gTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTa                                                                                                                               <  1:318136/65‑1 (MQ=255)

                                                                                              gCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc                                                                                                                                    >  1:291508/1‑45 (MQ=255)

                                                                                              gCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATc                                                                                                                                    <  2:291508/45‑1 (MQ=255)

                                                                                                 cATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTa                                                                                                                   >  1:458594/1‑59 (MQ=255)

                                                                                                 cATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTa                                                                                                                   <  2:458594/59‑1 (MQ=255)

                                                                                                  aTTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAAcg                                  <  2:1465964/139‑1 (MQ=255)

                                                                                                  aTTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCACCCTGTATTACTCCATGTATTGCCAAAATctctctct                                                                                                 >  1:184170/1‑76 (MQ=255)

                                                                                                  aTTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCACCCTGTATTACTCCATGTATTGCCAAAATctctctct                                                                                                 <  2:184170/76‑1 (MQ=255)

                                                                                                   tttGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTg                                                          >  1:1131546/1‑114 (MQ=255)

                                                                                                   tttGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTg                                                          <  2:1131546/114‑1 (MQ=255)

                                                                                                    ttGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTCTTACTCCATGTATTGCCAAAATCTCTCTCTGAATCTAATTACAGGTAACTGAAAAGAGAGATATTTTTGCAc                                                       <  2:980579/116‑1 (MQ=255)

                                                                                                    ttGGTTTTCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCAc                                                       >  1:980579/1‑116 (MQ=255)

                                                                                                         tttCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt                                                                                             >  1:1495270/1‑73 (MQ=255)

                                                                                                         tttCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTAt                                                                                             <  2:1495270/73‑1 (MQ=255)

                                                                                                           tCGGGCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAagaga                         <  2:1445555/139‑1 (MQ=255)

                                                                                                              ggCACCTTTTTCTGCTACTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATCTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGAc                      <  2:982516/139‑1 (MQ=255)

                                                                                                                               cTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATa                                                 <  2:586285/95‑1 (MQ=255)

                                                                                                                               cTTGAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATa                                                 >  1:586285/1‑95 (MQ=255)

                                                                                                                                  gAATGCATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATgaga  <  1:190184/139‑1 (MQ=255)

GCGAGCTTCAATAATCAATGCATCATAGGCGTTATTAACAGCACTCTTCGCCGCGGGACTCGCTGCTAAAAATGCGGCAGTAAGAAGTTTCAAAGCCCATTTGGTTTTCGGGCACCTTTTTCTGCTACTTGAATACATCCTGTATTACTCCATGTATTGCCAAAATCTCTCTCTGTATCTAATTACAGGTAACTGAAAAGAAAGATATTTTTGCACCTCATAATCCGTTATTAAACGCGGAAGAGAGACGTGAATTGTTGATGATGAGA  >  NC_000913/1092152‑1092420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A