Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A7 F1 I2 R1 | 766 | 64.2 | 3232166 | 90.9% | 2938038 | 104.5 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,117,958 | A→G | G173G (GGT→GGC) | yceI ← | periplasmic high pH‑inducible lipid‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,117,958 | 0 | A | G | 100.0% | 275.6 / NA | 76 | G173G (GGT→GGC) | yceI | periplasmic high pH‑inducible lipid‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (39/37); total (39/37) | |||||||||||
AACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCA > NC_000913/1117829‑1118093 | aacaCTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCt > 2:644758/1‑139 (MQ=255) tgtgCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCt > 2:1563151/1‑138 (MQ=255) tgtgCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCt < 1:1563151/138‑1 (MQ=255) tgtgCAGTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCtt > 1:1226645/1‑139 (MQ=255) tgCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt > 2:895405/1‑130 (MQ=255) tgCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt < 1:895405/130‑1 (MQ=255) tgCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGa > 2:1565934/1‑139 (MQ=255) tgCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGa > 1:258611/1‑139 (MQ=255) ccAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCt > 2:513578/1‑139 (MQ=255) aCAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt < 2:1357267/115‑1 (MQ=255) aCAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt > 1:1357267/1‑115 (MQ=255) aGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGc < 2:180696/139‑1 (MQ=255) aataTCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAAtt < 2:258611/139‑1 (MQ=255) aTTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt < 2:289009/101‑1 (MQ=255) aTTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt > 1:289009/1‑101 (MQ=255) aTTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCt < 2:1463477/107‑1 (MQ=255) aTTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCt > 1:1463477/1‑107 (MQ=255) gCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCg < 1:644758/139‑1 (MQ=255) ccGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt < 2:791313/97‑1 (MQ=255) ccGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt > 1:791313/1‑97 (MQ=255) ccGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCt < 1:1079741/118‑1 (MQ=255) ccGGATGCGTGCCATCCGGCAACATTTCACGCTTACTGCTGCTATACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCAGTCTTGATATTGAAGTCCt > 2:1079741/1‑118 (MQ=255) gATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCt < 1:1565934/139‑1 (MQ=255) attcGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCtt < 2:1571488/129‑1 (MQ=255) aTGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCtt > 1:1571488/1‑132 (MQ=255) tGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGc > 1:1389734/1‑139 (MQ=255) tCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTaaa > 2:1420967/1‑82 (MQ=255) tCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTaaa < 1:1420967/82‑1 (MQ=255) ccGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCAc < 2:1226645/139‑1 (MQ=255) cAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGa < 1:387659/93‑1 (MQ=255) cAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGa > 2:387659/1‑93 (MQ=255) cATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTacc > 1:208986/1‑139 (MQ=255) tACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt < 1:914060/53‑1 (MQ=255) tACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAAt > 2:914060/1‑53 (MQ=255) tACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCt > 2:159032/1‑98 (MQ=255) tACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCt < 1:159032/98‑1 (MQ=255) tACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGa > 1:12821/1‑139 (MQ=255) tACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGa < 2:12821/139‑1 (MQ=255) cGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGa < 2:701623/60‑1 (MQ=255) cGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGa > 1:701623/1‑60 (MQ=255) cGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGAcc > 2:1051117/1‑139 (MQ=255) cGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGAcc > 1:1486551/1‑139 (MQ=255) cTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCtt > 2:1080524/1‑87 (MQ=255) cTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCtt < 1:1080524/87‑1 (MQ=255) cTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTac < 2:116958/111‑1 (MQ=255) cTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTac > 1:116958/1‑111 (MQ=255) cTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGcc > 2:1316905/1‑130 (MQ=255) cTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGcc < 1:1316905/130‑1 (MQ=255) cTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCaat > 2:870863/1‑139 (MQ=255) tCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTg > 2:390148/1‑60 (MQ=255) tCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTg < 1:390148/60‑1 (MQ=255) tCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCt > 2:1517840/1‑99 (MQ=255) tCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCt < 1:1517840/99‑1 (MQ=255) taatCAGATCCACTTCCTGAGAAGCTGGGCCTAAATc > 1:232399/1‑37 (MQ=255) taatCAGATCCACTTCCTGAGAAGCTGGGCCTAAATc < 2:232399/37‑1 (MQ=255) atCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTg < 1:299069/42‑1 (MQ=255) atCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTg > 2:299069/1‑42 (MQ=255) atCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGAc < 1:389508/121‑1 (MQ=255) atCAGATCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGAc > 2:389508/1‑121 (MQ=255) aTCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTac > 1:1311258/1‑92 (MQ=255) aTCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTac < 2:1311258/92‑1 (MQ=255) aTCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTg < 1:1051117/139‑1 (MQ=255) tCCACTTCCTGAGAAGCTGGGCTTAAATCTGTCTTGATATTGAAGTCCTTGGGCTTAATTTGGCCTTCGGCCTCGAAGCCGGCACGTTTACCACCCCTGGGGTCGTGGCCCTGACCATTTAATTTCGCTT‑CAGCGTGAc < 2:353942/139‑1 (MQ=255) tCCACTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGa < 2:328200/139‑1 (MQ=255) aCTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGa < 1:830912/136‑1 (MQ=255) aCTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGa > 2:830912/1‑136 (MQ=255) cTTCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACggg > 1:602672/1‑139 (MQ=255) ttCCTGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTT‑CAGCGTGACGGGtt > 1:353942/1‑139 (MQ=255) tGAGAAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTgg < 1:513578/139‑1 (MQ=255) agaAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAAttt < 2:470923/114‑1 (MQ=255) agaAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAAttt > 1:470923/1‑114 (MQ=255) agaAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCa > 2:1014009/1‑122 (MQ=255) agaAGCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCa < 1:1014009/122‑1 (MQ=255) gCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGc < 2:1596967/139‑1 (MQ=255) gCTGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGc > 1:1596967/1‑139 (MQ=255) tGGGCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCa < 2:208986/139‑1 (MQ=255) | AACACTGTGCATTCCTGATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCA > NC_000913/1117829‑1118093 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |