Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	766	64.2	3232166	90.9%	2938038	104.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,513,468	T→C	S218P (TCC→CCC)	ydcT →	putative ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,513,468	0	T	C	100.0%	183.0 / NA	51	S218P (TCC→CCC)	ydcT	putative ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/30); total (21/30)

AGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATTCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAAC  >  NC_000913/1513337‑1513600

aGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcg                                                                                                                               <  1:1031195/139‑1 (MQ=255)

aGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcg                                                                                                                               <  2:119558/139‑1 (MQ=255)

  cTGTAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                             <  2:400450/139‑1 (MQ=255)

  cTGGAACTGAAAAAACTGCAAGAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGGGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGAGCCACcgcgcg                                                                                                                             <  1:1173986/139‑1 (MQ=255)

  cTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                             >  1:1133822/1‑139 (MQ=255)

  cTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                             <  2:11568/139‑1 (MQ=255)

      aaCTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGAtct                                                                                                                         >  2:989883/1‑139 (MQ=255)

               aaCTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATgcg                                                                                                                <  2:1271776/139‑1 (MQ=255)

               aaCTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATgcg                                                                                                                <  1:510801/139‑1 (MQ=255)

                       cAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCa                                                                                                        <  1:989883/139‑1 (MQ=255)

                         gTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACg                                                                                                      <  1:120545/139‑1 (MQ=255)

                                    tCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcg                                                                                                                               >  1:1398227/1‑103 (MQ=255)

                                    tCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcg                                                                                                                               <  2:1398227/103‑1 (MQ=255)

                                    tCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGc                                                                                           >  1:534096/1‑139 (MQ=255)

                                        ttttATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCggg                                                                                       <  1:526100/139‑1 (MQ=255)

                                          ttATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                                     <  1:1274135/139‑1 (MQ=255)

                                          ttATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                                     <  2:359488/139‑1 (MQ=255)

                                                tCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTgg                                                                               >  2:1294303/1‑139 (MQ=255)

                                                 cGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGt                                                                              >  1:364369/1‑139 (MQ=255)

                                                 cGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGt                                                                              <  2:1133822/139‑1 (MQ=255)

                                                    tACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGt                                                                                  >  1:1203757/1‑132 (MQ=255)

                                                    tACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGt                                                                                  <  2:1203757/132‑1 (MQ=255)

                                                    tACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACa                                                                           <  2:534096/139‑1 (MQ=255)

                                                    tACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACa                                                                           <  1:1294303/139‑1 (MQ=255)

                                                         aCGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGa                                                                       >  1:924891/1‑138 (MQ=255)

                                                         aCGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGa                                                                       <  2:924891/138‑1 (MQ=255)

                                                                gggCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGc                                                                                                     <  1:1471748/101‑1 (MQ=255)

                                                                gggCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGc                                                                                                     >  2:1471748/1‑101 (MQ=255)

                                                                gggCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                                     <  2:253277/117‑1 (MQ=255)

                                                                gggCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                                     >  1:253277/1‑117 (MQ=255)

                                                                  gCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGa                                                             <  1:994632/139‑1 (MQ=255)

                                                                           tATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGAt                                                    >  1:1234316/1‑139 (MQ=255)

                                                                                  gTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCcgcg                                                                                                          <  1:75212/78‑1 (MQ=255)

                                                                                  gTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCcgcg                                                                                                          >  2:75212/1‑78 (MQ=255)

                                                                                  gTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCagag                                             <  2:364369/139‑1 (MQ=255)

                                                                                    ccGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGaa                                           <  2:1234316/139‑1 (MQ=255)

                                                                                     cGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                             >  2:156357/1‑56 (MQ=255)

                                                                                     cGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCcgcgcg                                                                                                                             <  1:156357/56‑1 (MQ=255)

                                                                                               gCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                                     >  1:1558215/1‑86 (MQ=255)

                                                                                               gCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                                     <  2:1558215/86‑1 (MQ=255)

                                                                                                     ttCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                                     <  1:1212339/80‑1 (MQ=255)

                                                                                                     ttCAATAATGGACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGtt                                                                                     >  2:1212339/1‑80 (MQ=255)

                                                                                                               gACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGAt                                                            <  2:289055/95‑1 (MQ=255)

                                                                                                               gACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGAt                                                            >  1:289055/1‑95 (MQ=255)

                                                                                                               gACGCATTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGc                >  2:183601/1‑139 (MQ=255)

                                                                                                                    aTTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGt                                                                                                  >  1:427637/1‑52 (MQ=255)

                                                                                                                    aTTGAGCAGGTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGt                                                                                                  <  2:427637/52‑1 (MQ=255)

                                                                                                                             gTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAAc  >  2:339/1‑139 (MQ=255)

                                                                                                                             gTCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAAc  >  2:1214961/1‑139 (MQ=255)

                                                                                                                              tCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGa                                                                       >  2:1260512/1‑69 (MQ=255)

                                                                                                                              tCGATCCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGa                                                                       <  1:1260512/69‑1 (MQ=255)

AGCTGGAACTGAAAAAACTGCAACAGTCTCTCGGTATCACTTTTATCTTCGTTACCCACGATCAGGGCGAAGCGTTATCGATGTCCGATCGTGTGGCGGTTTTCAATAATGGACGCATTGAGCAGGTCGATTCCCCGCGCGATCTCTATATGCGCCCGCGCACGCCGTTTGTTGCCGGGTTCGTTGGTACATCGAATGTTTTTGATGGACTGATGGCAGAGAAACTTTGTGGCATGACGGGAAGCTTCGCCCTGCGACCGGAAC  >  NC_000913/1513337‑1513600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A